Deinococcus radiodurans, megaplasmid MP1

Source : TIGR

Chromosome 1

Chromosome 2

Megaplasmid MP1

Plasmid CP1

List of IS2621 copies, IRs , transposase alignment List of ISDra3 copies, IRs

List of ISDra1 copies, IRs , transposase alignment List of ISDra4 copies, IRs

List of ISDra2 copies, IRs , transposase alignment List of ISDra5 copies

List of ISDra6 copies, IRs

IS name

orf name

Simililarity DNA

Similarity AA

Family

orf L end

orf R end

orf bp

# aas

(1)
(2)
ISLend

ISRend

Length

DR

IR

IS coordinates present

Length

Comments

ISDra1D

DRB0005

ISDra1A(94%)

ISDra1B(99%)

IS4

6495

7478

984

327

t

6273

7479

1207

0

21/23
Mutation at position 159 K-->N.

IS2621H

IS2621(99%)

IS4

19103

20353

1251

416

19049

20370

1208

0

19

1322
Mutations at positions 1-3 MNT-->VKS, 19 A-->T, 46 V-->A, 69 P-->D, 145 F-->L, 153 R-->L, 165 deletion of A, 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 254-256 ETS-->ARP, 338 P-->Q, 363 E-->G and 390 K-->N.
Point mutation at position 400 causing a premature stop.

ISDra1E

DRB0020

ISDra1A(94%)

ISDra1B(99%)

IS4

22659

21676

984

327

c

t

22881

21675

1207

0

21/23
Mutation at position 239 M-->I.

IS2621-P

DRB0055

IS2621(99%)

IS2621(91%)

IS4

64051

63851

c

t

63834

64000(1156)-63834(1322)

167
End of the transposase (368 to 416).
Mutation at position 390 K-->N, 401-405 EHLPS-->ANSPF.

ISDra1F'

ISDra1A(95%)

IS4

64001

0

21/23

64000(1)-64199(200)

200
Disrupted.

ISDra6B

DRB0056

ISDra6A(99%)

IS1741(54%)

IS630

64248

65259

1012

336

t

64202

65273

1072

0

19/21
Frameshift

ISDra1F''

DRB0057

ISDra1A(91%)

ISDra1B(98%)

IS4

65291

66274

984

327

t

66276

65281(211)-66275(1207)

998

Mutations at positions 3 A-->D and 4-5 RS-->PR.

IS2621-P

DRB0058

IS2621(99%)

IS2621(93%)

IS4

66255

67502

1248

415

t

none

67519

66276(79-67519(1322)

1244
Transposase (6 to 416).
Mutation at position 7-8 MG-->TE, 46 V-->A, 69 P-->D, 135 A-->T, 145 F-->L, 153 R-->L, 165 A-->T, 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 254-256 ETS-->ARP, 338 P-->Q, 363 E-->G and 390 K-->N.
Point mutation at position 400 causing a premature stop.

IS2621I

DRB0059

IS2621(89%)

IS2621(100%)

IS4

67669

68919

1251

416

t

67615

68936

1322

7

19

1322

IS2621-P

IS2621(100%)

IS4

c

121585

121585(1)-121566(20)

20

ISDra1G

DRB0102

ISDra1A(94%)

ISDra1B(99%)

IS4

122839

121856

984

327

c

t

123063

121853

1207

0

21/23

Mutation at position 159 K-->N.

IS2621-P

DRB0103

IS2621(99%)

IS2621(94%)

IS4

123011

124240

1230

410

t

none

124305

123062(79)-124305(1322)

1244
Transposase (6 to 400).
Mutation at position 7-8 MG-->TE, 46 V-->A, 69 P-->D, 135 A-->T, 145 F-->L, 153 R-->L, 165 A-->T, 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 254-256 ETS-->ARP, 338 P-->Q, 363 E-->G and 390 K-->N.
Point mutation at position 400 causing a premature stop.

ISDra6C

DRB0113

ISDra6A(99%)

IS1741(54%)

IS630

133761

134772

1012

336

t

133715

134786

1072

0

19/21
Frameshift

IS2621J

DRB0114

IS2621(99%)

IS2621(94%)

IS4

135452

136141

690

230

t

134885

136206

1322

0

19

1322
End of the transposase (172 to 400), mutations at positions 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 253-255 ETS-->ARP, 338 P-->Q, 363 E-->G and 391 K-->N.

Our translation

134939

136189

1251

416
Mutations at positions 1-3 MNT-->VKS, 19 A-->T, 46 V-->A, 69 P-->D, 145 F-->L, 153 R-->L, 165 deletion of A, 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 254-256 ETS-->ARP, 338 P-->Q, 363 E-->G and 390 K-->N.
Point mutation at position 400 causing a premature stop.

ISDra1H

DRB0117

ISDra1A(93%)

ISDra1B(98%)

IS4

137627

138610

984

327

t

137405

138611

1207

0

21/23

137405(2)-138613(1209)
Mutations at positions 310 F-->L, 313 A-->P, 315 C-->S and 321 E-->G.

ISDra4E

DRB0120

ISDra (98%)

IS630

142149

141141

1009

335

c

t

142189

141142

1048

0

19/25
No translation available. Authentic frameshift

IS2621K

DRB0132

IS2621(99%)

IS2621(94%)

IS4

153555

152866

690

230

c

t

154122

152801

1322

0

19

1322
End of the transposase (172 to 400), mutations at positions 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 253-255 ETS-->ARP, 338 P-->Q, 363 E-->G and 391 K-->N.

Our translation

1251

416
Mutations at positions 1-3 MNT-->VKS, 19 A-->T, 46 V-->A, 69 P-->D, 145 F-->L, 153 R-->L, 165 deletion of A, 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 254-256 ETS-->ARP, 338 P-->Q, 363 E-->G and 390 K-->N.
Point mutation at position 400 causing a premature stop.

ISDra1I

DRB0134

ISDra1A(93%)

ISDra1B(98%)

IS4

155958

156941

984

327

t

155736

156942

1207

0

21/23

155736(2)-156942(1207)
Mutations at positions 3 A-->D, 159 K-->N and 316 P-->L.

ISDra3B

DRB0139

ISDra3A(99%)

IS630

168659

169643

985

327

t

168588

169657

1070

0

13/14
No translation available. Authentic frameshift

IS name	orf name	Simililarity DNA	Similarity AA	Family	orf L end	orf R end	orf bp	# aas	(1)	(2)	ISLend	ISRend	Length	DR	IR	IS coordinates present	Length	Comments
ISDra1D	DRB0005	ISDra1A(94%)	ISDra1B(99%)	IS4	6495	7478	984	327		t	6273	7479	1207	0	21/23			Mutation at position 159 K-->N.
IS2621H		IS2621(99%)		IS4	19103	20353	1251	416			19049	20370	1208	0	19		1322	Mutations at positions 1-3 MNT-->VKS, 19 A-->T, 46 V-->A, 69 P-->D, 145 F-->L, 153 R-->L, 165 deletion of A, 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 254-256 ETS-->ARP, 338 P-->Q, 363 E-->G and 390 K-->N. Point mutation at position 400 causing a premature stop.
ISDra1E	DRB0020	ISDra1A(94%)	ISDra1B(99%)	IS4	22659	21676	984	327	c	t	22881	21675	1207	0	21/23			Mutation at position 239 M-->I.
IS2621-P	DRB0055	IS2621(99%)	IS2621(91%)	IS4	64051	63851			c	t		63834				64000(1156)-63834(1322)	167	End of the transposase (368 to 416). Mutation at position 390 K-->N, 401-405 EHLPS-->ANSPF.
ISDra1F'		ISDra1A(95%)		IS4							64001			0	21/23	64000(1)-64199(200)	200	Disrupted.
ISDra6B	DRB0056	ISDra6A(99%)	IS1741(54%)	IS630	64248	65259	1012	336		t	64202	65273	1072	0	19/21			Frameshift
ISDra1F''	DRB0057	ISDra1A(91%)	ISDra1B(98%)	IS4	65291	66274	984	327		t		66276				65281(211)-66275(1207)	998	Mutations at positions 3 A-->D and 4-5 RS-->PR.
IS2621-P	DRB0058	IS2621(99%)	IS2621(93%)	IS4	66255	67502	1248	415		t	none	67519				66276(79-67519(1322)	1244	Transposase (6 to 416). Mutation at position 7-8 MG-->TE, 46 V-->A, 69 P-->D, 135 A-->T, 145 F-->L, 153 R-->L, 165 A-->T, 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 254-256 ETS-->ARP, 338 P-->Q, 363 E-->G and 390 K-->N. Point mutation at position 400 causing a premature stop.
IS2621I	DRB0059	IS2621(89%)	IS2621(100%)	IS4	67669	68919	1251	416		t	67615	68936	1322	7	19		1322
IS2621-P		IS2621(100%)		IS4					c		121585					121585(1)-121566(20)	20
ISDra1G	DRB0102	ISDra1A(94%)	ISDra1B(99%)	IS4	122839	121856	984	327	c	t	123063	121853	1207	0	21/23			Mutation at position 159 K-->N.
IS2621-P	DRB0103	IS2621(99%)	IS2621(94%)	IS4	123011	124240	1230	410		t	none	124305				123062(79)-124305(1322)	1244	Transposase (6 to 400). Mutation at position 7-8 MG-->TE, 46 V-->A, 69 P-->D, 135 A-->T, 145 F-->L, 153 R-->L, 165 A-->T, 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 254-256 ETS-->ARP, 338 P-->Q, 363 E-->G and 390 K-->N. Point mutation at position 400 causing a premature stop.
ISDra6C	DRB0113	ISDra6A(99%)	IS1741(54%)	IS630	133761	134772	1012	336		t	133715	134786	1072	0	19/21			Frameshift
IS2621J	DRB0114	IS2621(99%)	IS2621(94%)	IS4	135452	136141	690	230		t	134885	136206	1322	0	19		1322	End of the transposase (172 to 400), mutations at positions 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 253-255 ETS-->ARP, 338 P-->Q, 363 E-->G and 391 K-->N.
Our translation		134939	136189	1251	416								Mutations at positions 1-3 MNT-->VKS, 19 A-->T, 46 V-->A, 69 P-->D, 145 F-->L, 153 R-->L, 165 deletion of A, 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 254-256 ETS-->ARP, 338 P-->Q, 363 E-->G and 390 K-->N. Point mutation at position 400 causing a premature stop.
ISDra1H	DRB0117	ISDra1A(93%)	ISDra1B(98%)	IS4	137627	138610	984	327		t	137405	138611	1207	0	21/23	137405(2)-138613(1209)		Mutations at positions 310 F-->L, 313 A-->P, 315 C-->S and 321 E-->G.
ISDra4E	DRB0120	ISDra (98%)		IS630	142149	141141	1009	335	c	t	142189	141142	1048	0	19/25			No translation available. Authentic frameshift
IS2621K	DRB0132	IS2621(99%)	IS2621(94%)	IS4	153555	152866	690	230	c	t	154122	152801	1322	0	19		1322	End of the transposase (172 to 400), mutations at positions 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 253-255 ETS-->ARP, 338 P-->Q, 363 E-->G and 391 K-->N.
Our translation				1251	416								Mutations at positions 1-3 MNT-->VKS, 19 A-->T, 46 V-->A, 69 P-->D, 145 F-->L, 153 R-->L, 165 deletion of A, 180 P-->Q, 197 S-->P, 213 F-->L, 220-221 KY-->ND, 234 P-->K, 254-256 ETS-->ARP, 338 P-->Q, 363 E-->G and 390 K-->N. Point mutation at position 400 causing a premature stop.
ISDra1I	DRB0134	ISDra1A(93%)	ISDra1B(98%)	IS4	155958	156941	984	327		t	155736	156942	1207	0	21/23	155736(2)-156942(1207)		Mutations at positions 3 A-->D, 159 K-->N and 316 P-->L.
ISDra3B	DRB0139	ISDra3A(99%)		IS630	168659	169643	985	327		t	168588	169657	1070	0	13/14			No translation available. Authentic frameshift

Bold is for complete copies of an IS
Italic is for partial copies of an IS (1)"c" is for complementary strand
(2)"t" is for transposase

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