Influence of transposition
mechanisms on genome impact
The way in which strand
cleavages and transfers occur during transposition also affects the outcome of
the transposition events and therefore impinges on genome structure. For IS
with DDE Tpases, Tn3 and IS6 family members generate fusions or
cointegrates between the donor and target replicons by a process of replicative
transposition, presumably by TPRT (Target primed replicative transposition) (Hickman, et al., 2010, Hickman & Dyda,
2015, Siguier, et al., 2015) (Figs 1.23.1 and 1.23.2 top). However, in the event of intramolecular
transposition, this type of mechanism is expected to give rise to inversions
with a copy of the IS at each junction or inversions with a single IS copy
remaining and a second copy segregating with a circularized deletion (Shapiro, 1979) (Fig 1.23.2 bottom; see (He, et al., 2015)). Note
that similar effects are also known to occur by homologous recombination
between two inverted or directly repeated IS copies in a replicon. Other known
mechanisms such as cut-and-paste, or copy-and-paste (DPRT, Donor primed replicative transposition) (Curcio & Derbyshire, 2003) would not
generate this type of genomic rearrangement but could contribute to genomic
modifications in other ways such as "nearly precise excision" (Ross, et al.,
1979, Ross, et al., 1979) or
by using alternative sequences which resemble their IR (Ohtsubo, et al., 1980, Polard, et al., 1994).
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DG, Swan J & Kleckner N (1979) Nearly precise excision: a new type of DNA
alteration associated with the translocatable element Tn10. Cell 16: 733-738.
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