ISAzo4

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Azoarcus sp. EbN1
DNA section
IS Length : 2757 bp

Ends


IR Length : 26/29

IRL : TGCGGATTCCGACCCAACGTGACCGCTGACTCCGAAATAGTGTGACCGGT
IRR : TGCGCATTCCGACGAACGTGACCGCCTGCACCGACGAACGTGACCGGGGG

Insertion site


Left flankDirect repeatRight flankDR Length
GAAGGCCATCGGCCAGCCGGCGGGGCT7

DNA sequence

TGCGGATTCCGACCCAACGTGACCGCTGACTCCGAAATAGTGTGACCGGTGAATCCGCGGTCGTGACCGCGGATTCCGATTTGATCGTGACCGATTTCGG
CGAGTTGTCGGAATGGGCGGTCACGATGTCGGAATCAATGGTCACGATCAAATCGGAACGGGTTTGTGAGGCCAAGCGTGGCAACGTCGTTTGTCAGGCC
GGCTACCCTCGCGCGCTTTGCGCGGAGACCGAGGATGCCGGCGGAGCGGATTGCCATGCACAAGATCAGGGAGCTGTTACGGCTCAAATACGACTGCGCG
CTGTCGCATGAGCGCATTGCCCGCGCGCTGTCGATTTCCAAAGGCGTGGTCGCCAAGTATGTGAAGGCGGCCGAGGAGTGCGGCCGGCCGTGGGCCGAGT
TGTCGGCGGCCGACGAGGCCGAGTTGCGCCGGGTGCTGGGGGTCGCCCGGCGTGGACGTGGCGCGAGCGTCGCGAATGTGCCGCCGGATCTGGCGGCGGT
GCATCAGGGGCTCAAGCGAAAGAACGTCACGCTGGCGCTGCTGTGGGAAGAGTACGTGCAGACGGCCGACGGGCCGAGCTATCAGTACTCGCGCTTTTGC
GACCTGTACCGCGCGTTCGCCCGCACGCTCAAGCGCTCGATGCGCCAGGTGCACCGCGCCGGCGAGAAACTCTTCATCGATTACGCGGGCGACACGGTGC
CGATCGTCGACGCCGACACGGGCGAGATTTCGCGCGCGCAGATCTTCGTCGCGGTGCTCGGCGCCTCGAGCTATACGTTCGCCTGCGCCACGGCGACGCA
GTCGCAGGCCGACTGGCTGGGCTCGCTCGCCCGGGCGCTGGCCTTCATCGGCGGCGTGCCCGAGCTCGTGGTGCCCGACAATACGCGCTCGCTCGTTGGC
CAAGCCGACCGCTACGAGCCGCAACTGCAGCGCACCACCGCCGAGTTCGCCGCGCACTACGGCGTGGCGATCCTGCCCGCGCGCCCCTACAAGCCGCAGG
ACAAGGCCAAGGTCGAAGTCGGCGTACAGATCGTGCAGCGCTGGATTCTGGCGCGGCTGCGGCATCGACGTTTCTTCTCGCTGGGAGAGTTGAACGAGGC
GATCGCCGCGTTACTCGAGCCGCTGAACACGCGTGCCTTCCGGCGCCTGCCAGGCTCGCGCCACGAAGCGTTCGAGACGCTCGATCGGCCGGCGCTGCGC
CCGTTGCCCGCCACCGCGTTCCAGTTCGCCCAGTGGAAACGGGCCAAGCCCAATATCGATTACCACGTCGAGTTCGACGGGCATTACTACAGCGTGCCGT
ATGCGCTCGCCGGCCAACCGGTGGAGTTGCGCATCACCGCGAGCAGCATCGAATGCTTTGCGGCGGGCCGCCGCGTCGCGGTGCATGCGAGAAGCCCCCG
GTACGGAGCGTTCACCACGCTCACCGAGCACATGCCCGCATCCCATCAGGCGCATCGCCAGTGGTCCCCGGGCAAACTCATCGCCTGGGGTGCCACGGTC
GGACCTCACACCGAGCAGGTGGTCAGCCACCAACTCGAACGCATGCCGCACCCCGAGCAGGGCTACCGGGCGTGCCTCGGGCTGATGCGCCTCGGTCGCC
AATACGGCAACGAACGCCTCGAAGCCGCGGCGACCCGCGCCGTCACCCTCGGGGCGATGCGTTACCGCAACGTCGCCTCGATCCTCAAGAGCGGGCTCGA
CCGCGCGCCGCTCCCCGCATCCACTGCGCAGCAAAGCGAGCTCGCGCTACCGGCCGCTCACGAGAACCTGCGCGGGGCGCGCTACTACCACTGATTCCAC
CCACCGGAGAACATCGATGCTGATACAACACAGCCTGCAACAACTGCGCACCCTGCGCCTGGAAGGCATGGCGCGCGCCTTCGAGGAGCAGCTCACCCAG
CCCGCCATCACCGCGCTCAGCTTCGAGGAGCGCTTCGCCCAGCTCATCGATCGCGAGATCCTGCTGCGCGACGGCAAACGCATCGATCGGCTGCTCAAGG
CCGCCCGCATCAAAGCCGCTGCCGCCTGCCTGGAGGACGTCGACTACCGCGCCGGGCGCGGCCTCGAGCGCAGTCAGATCGCCGCGCTCGGAACCGGCCA
GTGGATTCGCCACCACCAGAACTGCCTCATCACGGGCCCCACCGGCAGCGGCAAGACGTGGCTCGCCTGCGCGCTCGCCAACGCGGCGTGCCGGCAGGGC
CTCGCGGCCTACTACGTGCGTCTGCCGCGGCTCTTCGAGGAGCTGCGCATCGCGCATGCCGATGGCAGCTTCAGCCGACGCCTCATGCAGCTCGCGCGCA
TGGATCTCATCGTCATCGACGATTGGGGCCTGGCCGCGCCGTCGGCGCAGGAGCGAAGCGACCTGCTGGAGCTGCTCGACGACCGCGTCGGCACGCGTTC
GACCGTGATCACCAGCCAGCTGCCGATCGAGCACTGGCACACCTACCTGGGCGACCCGACCTTCGCCGATGCGATCCTCGATCGCGTCGTGCACGCCGCG
CACAAGCTCGCCCTCAAGGGCGAGTCGATGAGAAGAAAGGAAAAGGCATGAGGCCGTGCGCGCGAGACGCCTTACCCACAGGCGCCCGCCCGCCAGCGTA
TGAGGCGCGCTGGCGGCCGCCTGTGGATAAGCCTGCGCCGTGCCCCGAATTGACCGTGATCGTGACCGACACGGTTAGAATCTGAACACCACGCTTCGCG
CGCAGACCCCCCGGTCACGTTCGTCGGTGCAGGCGGTCACGTTCGTCGGAATGCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1560 bp519 aa2351794+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPAERIAMHKIRELLRLKYDCALSHERIARALSISKGVVAKYVKAAEECGRPWAELSAADEAELRRVLGVARRGRGASVANVPPDLAAVHQGLKRKNVTL
ALLWEEYVQTADGPSYQYSRFCDLYRAFARTLKRSMRQVHRAGEKLFIDYAGDTVPIVDADTGEISRAQIFVAVLGASSYTFACATATQSQADWLGSLAR
ALAFIGGVPELVVPDNTRSLVGQADRYEPQLQRTTAEFAAHYGVAILPARPYKPQDKAKVEVGVQIVQRWILARLRHRRFFSLGELNEAIAALLEPLNTR
AFRRLPGSRHEAFETLDRPALRPLPATAFQFAQWKRAKPNIDYHVEFDGHYYSVPYALAGQPVELRITASSIECFAAGRRVAVHARSPRYGAFTTLTEHM
PASHQAHRQWSPGKLIAWGATVGPHTEQVVSHQLERMPHPEQGYRACLGLMRLGRQYGNERLEAAATRAVTLGAMRYRNVASILKSGLDRAPLPASTAQQ
SELALPAAHENLRGARYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
735 bp244 aa18172551+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLIQHSLQQLRTLRLEGMARAFEEQLTQPAITALSFEERFAQLIDREILLRDGKRIDRLLKAARIKAAAACLEDVDYRAGRGLERSQIAALGTGQWIRHH
QNCLITGPTGSGKTWLACALANAACRQGLAAYYVRLPRLFEELRIAHADGSFSRRLMQLARMDLIVIDDWGLAAPSAQERSDLLELLDDRVGTRSTVITS
QLPIEHWHTYLGDPTFADAILDRVVHAAHKLALKGESMRRKEKA

 

Blast result :
Comments
ISAzo4 is 70% (ORF1) and 80% (ORF2) aa simialr to IS408. There are 16 copies in Azoarcus EbN1 genome.
References
1] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36