ISKpn3

  • Family IS1595
  • Group IS1595
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AJ971342 ND Klebsiella pneumoniae
Klebsiella pneumoniae plasmid pRDDHA
DNA section
IS Length : 1031 bp

Ends


IR Length : 21/26

IRL : CCTGATTACCCATGATTAGCAGTCAGTTACCACGAACTATACTGATCTTA
IRR : CCTGATTACCCATGACCCTCAGCCAGCTTCAAGAGCCGATATGGCATCGG

Insertion site


Left flankDirect repeatRight flankDR Length
TTGCCCCTCTTAAGGTCAAGTCTCTTTC8
GAACCCCTCAAAGGGGATTGACCAAGAG8
CTCGATAATAAAGCGATTTAGGGCAAGC8
TGGTGAGTTTTTCCCTGATAAAGATAAC8
CTAAATGATAAAAGCTTTTG0

DNA sequence

CCTGATTACCCATGATTAGCAGTCAGTTACCACGAACTATACTGATCTTACGTACAGTTATCGAGGTGCGAGATCATGGCCAAAAGCAAAATCCAATTCC
AGAAAGGCTACAGCCTGTTTGAGTTTCTCAAGGACTATGGCACTGAGGCTCAGTGCGAAAACGCGCTGTTCACTTGGCGTTTTCCTAACGGCTTTGTCTG
CCCTGAATGTGCCAACAAGACCTGTTGTCGCCTGAGAAGGCGGCCACGTGTGTGGCAATGCAATCACTGCCGTCACCAGATGTCATTGACTGGAAACACG
ATTTTCGCAAACACCAAGCTCCCACTGACCAAGTGGTTTCTGGCAATGCACCTGTTGTCACAAACCAAAACAGGCTTGTCAGCGATGGAGCTAAAGCGCC
AGTTGGGCGTCAAATATGACACCGCCTGGATGCTCAAGCACAAGCTGCTGCAAGCCATGAAAGAGTCCGATGACAAGCATTCCATCAGCGGCATCATTCA
GCTCGATGATGTCTACTGGGGTGGCGAGCGCCGTGGTGGCAAACGGGGTCGGGGCGCGGCAGGCAAGACGCCGTTTGTGGCAGCAGTGGCCCTGAACAAG
GAAGGACATCCAATCAAGATGCGGATGACGGTGGTCGATGGTTTCAAAACCAAGTCCGTTGCTGATTGGGCAAAACGGCATATTGCCAGTGGTAGCGCGG
TGATATCCGATGGATTGGCCTGTTTCAAAGCGGTCAAGGAGGCCAATTGTGAGCACCTGGGTGTTGTCACCGGGGGCAACCTGGACTGGCTGGATCATCC
GGCATTCAACTGGGTAAACACGATGATAGGAAATGTGAAAAACTCGCTGCGGGGCAGTTGCCATACGCTCGGAGCGAAGCATTTACCAAGGCATCTTGCG
GAATACTGCTTCCGGTTTAATCATCGTTTTGACCTCAAGAGCATGTTGGTTGAATTGGGGCATGCAGTCGTGGCAAGCCCTCCGATGCCATATCGGCTCT
TGAAGCTGGCTGAGGGTCATGGGTAATCAGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
951 bp316 aa761026+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAKSKIQFQKGYSLFEFLKDYGTEAQCENALFTWRFPNGFVCPECANKTCCRLRRRPRVWQCNHCRHQMSLTGNTIFANTKLPLTKWFLAMHLLSQTKTG
LSAMELKRQLGVKYDTAWMLKHKLLQAMKESDDKHSISGIIQLDDVYWGGERRGGKRGRGAAGKTPFVAAVALNKEGHPIKMRMTVVDGFKTKSVADWAK
RHIASGSAVISDGLACFKAVKEANCEHLGVVTGGNLDWLDHPAFNWVNTMIGNVKNSLRGSCHTLGAKHLPRHLAEYCFRFNHRFDLKSMLVELGHAVVA
SPPMPYRLLKLAEGHG

 

Blast result :
Comments
ISKpn3 is 60% aa similar to ISXo2.
References
1] Verdet,C., Benzerara,Y., Gautier,V., Adam,O., Ould-Hocine,Z. and
Arlet,G. (2006) Antimicrob. Agents Chemother. 50 (2), 607-617