ISAba8
- Family IS21
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
ND | Acinetobacter baumannii | Acinetobacter baumannii |
DNA section
IS Length : 1955 bp
Ends
IR Length : 27
IRL : TGTTAGCGGCAGTGTAAAAATGACCCCCTAATGGCATTTAAAAACTGACC
IRR : TGTCAACGGCATTCTAAAATTGACCCCTTTTACCTTACAAATGGCAAAGT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
AATATCGAAT | CCAT | GAACAGTGGT | 4 |
DNA sequence
TGTTAGCGGCAGTGTAAAAATGACCCCCTAATGGCATTTAAAAACTGACCCCCTTGGTTAATATAGCGGTCATTTTGGACTGCTCAACATGTTAACTTTG
GAGCAAGCAGTGACAATCCAAATACTTCATCAGCAAGGTAAATCTATTAAAGCTATTACTCGTGAACTGGGGGTGTCCAGAAATACCGTACGTAAATATT
TACGGCAAAACACCACACCTCAGTATCAGCGTATACAGCCTAGAATAAGTATCCTTGACCCATATAAACCCTATTTACTCCAACGTGTAAACGCAGCTCA
TCCTGAATGGATTCCTGCTGTTGTGCTCTACCAGGAAATTTTAGGGTTGGGATATCCAGGAAAGATCAGGATCTTGAGGGAATATCTTGCAACATTAAAA
CCAGTTGCCAAACCGGAACCGATCATTCGTTTTGAAACCCAACCTGGCCAACAAATGCAGGTGGATTTCACTACGATTCGACGCAACAACACGACTTTAA
AAGCCTTTGTCGCAACATTAGGGTATTCCAGAGCGACTTTCGTGAAATTTTATGATCATGAACGTACGGATGCATGGATCGATGGTCTAGAAAATGCATT
TCAGTTCTTTGCAGGAGTACCTCAAGAAATCCTGTTTGATAACGCTAAAACGATCATGATTGAACGGGATGCCTATCAGGAGGGGCAGCATAAATGGAAC
CCCAAACTGCTCGACTGCGCCAAGAAGTACAGCTTTCGTCCTCGCGTATGTAAGCCCTACCGTGCACAGACCAAAGGCAAAGTTGAACGCTTTAATGGAT
ACCTCAAATCAAGCTTTATTGTGCCATTGAAAGCAAGCCTGAAGACTTCGGGTTTACTGTTAGATGTTGATGTCGCCAATGCCCACATTGGCCGGTGGCT
GCATGAAACCGCCAATCAGCGGATTCATGCCACCACGCAAGAAAAACCGGCTGTTCGACTGCAACAGGAGCAGCAAAAATTTACGCCATTACCGCAATCA
GATACAGGTTCTAGCACTGTACCTGTTGCAGCAGCACAGCATGTCATGCCCTACGAGAGTTTGCAGCATCCCTTATCGGTATATGACCAGTTGCTGGGAG
TTTCCTGATGAATCTGCAATACGACCGTATAGAGCAGCTTTGCCAAAAGCTCAATCTGCCTGCCATCGCATCACAATGGTCACATCATGCACAACAAACA
TTAGGTCAAGATGGAAGTTATGCCGACTTTGTTGAAGCGATCTTGACGCATGAATATGCTGCCAGGCAACAGCGCAGTCAGCAGGTACTGATGAAACTCT
CAGGCCTGCCTCAAGTCAAAACCCTGGAAGACTATGATTTTAATTATGCCCTAGGGGCCCCTAAATCACAGGTGATTGAACTGTTCAATCTGAGCTTTAT
TGCTAGAGCAGAAAATGTGGTGTTTCTCGGGGCTAGCGGAGTAGGAAAAACGCACTTATCTATGGCATTAGGCTATAAGGCCATCATGAACAACATTAAG
ATCAAGTTCATTACCGCAGCGGATTTAATGCTGCAACTGAGTACAGCCTATCAGCAAGGTAAATTGAAAACCTATATGCAGCGTGCGGTACTGGGACCCA
AGTTACTGATTATCGATGAAATTGGTTATTTACCGTTTGGACGTGAAGAAGCGAATCTGTTCTTTAACGTGATTGCCAAGCGTTATGAAAAAGGCAGTAC
GATATTGACGAGTAACTTACCCTTTAGCCAGTGGTCCAAATCCTTTGCAGATGATGTCACGTTGACCGCTGCGATGTTAGATCGGCTATTACATCACTGT
CATGTGGTACAAATATCGGGTGAGAGTTATCGTTTGAAGGATAGAAAAAGAATTGGGATTCAACCCCAGGTTGAAACTTAATACGAGATCAAAGGGGTCA
ATTTTACTTTGCCATTTGTAAGGTAAAAGGGGTCAATTTTAGAATGCCGTTGACA
GAGCAAGCAGTGACAATCCAAATACTTCATCAGCAAGGTAAATCTATTAAAGCTATTACTCGTGAACTGGGGGTGTCCAGAAATACCGTACGTAAATATT
TACGGCAAAACACCACACCTCAGTATCAGCGTATACAGCCTAGAATAAGTATCCTTGACCCATATAAACCCTATTTACTCCAACGTGTAAACGCAGCTCA
TCCTGAATGGATTCCTGCTGTTGTGCTCTACCAGGAAATTTTAGGGTTGGGATATCCAGGAAAGATCAGGATCTTGAGGGAATATCTTGCAACATTAAAA
CCAGTTGCCAAACCGGAACCGATCATTCGTTTTGAAACCCAACCTGGCCAACAAATGCAGGTGGATTTCACTACGATTCGACGCAACAACACGACTTTAA
AAGCCTTTGTCGCAACATTAGGGTATTCCAGAGCGACTTTCGTGAAATTTTATGATCATGAACGTACGGATGCATGGATCGATGGTCTAGAAAATGCATT
TCAGTTCTTTGCAGGAGTACCTCAAGAAATCCTGTTTGATAACGCTAAAACGATCATGATTGAACGGGATGCCTATCAGGAGGGGCAGCATAAATGGAAC
CCCAAACTGCTCGACTGCGCCAAGAAGTACAGCTTTCGTCCTCGCGTATGTAAGCCCTACCGTGCACAGACCAAAGGCAAAGTTGAACGCTTTAATGGAT
ACCTCAAATCAAGCTTTATTGTGCCATTGAAAGCAAGCCTGAAGACTTCGGGTTTACTGTTAGATGTTGATGTCGCCAATGCCCACATTGGCCGGTGGCT
GCATGAAACCGCCAATCAGCGGATTCATGCCACCACGCAAGAAAAACCGGCTGTTCGACTGCAACAGGAGCAGCAAAAATTTACGCCATTACCGCAATCA
GATACAGGTTCTAGCACTGTACCTGTTGCAGCAGCACAGCATGTCATGCCCTACGAGAGTTTGCAGCATCCCTTATCGGTATATGACCAGTTGCTGGGAG
TTTCCTGATGAATCTGCAATACGACCGTATAGAGCAGCTTTGCCAAAAGCTCAATCTGCCTGCCATCGCATCACAATGGTCACATCATGCACAACAAACA
TTAGGTCAAGATGGAAGTTATGCCGACTTTGTTGAAGCGATCTTGACGCATGAATATGCTGCCAGGCAACAGCGCAGTCAGCAGGTACTGATGAAACTCT
CAGGCCTGCCTCAAGTCAAAACCCTGGAAGACTATGATTTTAATTATGCCCTAGGGGCCCCTAAATCACAGGTGATTGAACTGTTCAATCTGAGCTTTAT
TGCTAGAGCAGAAAATGTGGTGTTTCTCGGGGCTAGCGGAGTAGGAAAAACGCACTTATCTATGGCATTAGGCTATAAGGCCATCATGAACAACATTAAG
ATCAAGTTCATTACCGCAGCGGATTTAATGCTGCAACTGAGTACAGCCTATCAGCAAGGTAAATTGAAAACCTATATGCAGCGTGCGGTACTGGGACCCA
AGTTACTGATTATCGATGAAATTGGTTATTTACCGTTTGGACGTGAAGAAGCGAATCTGTTCTTTAACGTGATTGCCAAGCGTTATGAAAAAGGCAGTAC
GATATTGACGAGTAACTTACCCTTTAGCCAGTGGTCCAAATCCTTTGCAGATGATGTCACGTTGACCGCTGCGATGTTAGATCGGCTATTACATCACTGT
CATGTGGTACAAATATCGGGTGAGAGTTATCGTTTGAAGGATAGAAAAAGAATTGGGATTCAACCCCAGGTTGAAACTTAATACGAGATCAAAGGGGTCA
ATTTTACTTTGCCATTTGTAAGGTAAAAGGGGTCAATTTTAGAATGCCGTTGACA
Protein section
ORF number : 2
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1020 bp | 339 aa | 89 | 1108 | + | No |
Chemistry : DDE
ORF sequence :
MLTLEQAVTIQILHQQGKSIKAITRELGVSRNTVRKYLRQNTTPQYQRIQPRISILDPYKPYLLQRVNAAHPEWIPAVVLYQEILGLGYPGKIRILREYL
ATLKPVAKPEPIIRFETQPGQQMQVDFTTIRRNNTTLKAFVATLGYSRATFVKFYDHERTDAWIDGLENAFQFFAGVPQEILFDNAKTIMIERDAYQEGQ
HKWNPKLLDCAKKYSFRPRVCKPYRAQTKGKVERFNGYLKSSFIVPLKASLKTSGLLLDVDVANAHIGRWLHETANQRIHATTQEKPAVRLQQEQQKFTP
LPQSDTGSSTVPVAAAQHVMPYESLQHPLSVYDQLLGVS
ATLKPVAKPEPIIRFETQPGQQMQVDFTTIRRNNTTLKAFVATLGYSRATFVKFYDHERTDAWIDGLENAFQFFAGVPQEILFDNAKTIMIERDAYQEGQ
HKWNPKLLDCAKKYSFRPRVCKPYRAQTKGKVERFNGYLKSSFIVPLKASLKTSGLLLDVDVANAHIGRWLHETANQRIHATTQEKPAVRLQQEQQKFTP
LPQSDTGSSTVPVAAAQHVMPYESLQHPLSVYDQLLGVS
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
774 bp | 257 aa | 1108 | 1881 | + | No |
AG : IS21 helper
ORF sequence :
MNLQYDRIEQLCQKLNLPAIASQWSHHAQQTLGQDGSYADFVEAILTHEYAARQQRSQQVLMKLSGLPQVKTLEDYDFNYALGAPKSQVIELFNLSFIAR
AENVVFLGASGVGKTHLSMALGYKAIMNNIKIKFITAADLMLQLSTAYQQGKLKTYMQRAVLGPKLLIIDEIGYLPFGREEANLFFNVIAKRYEKGSTIL
TSNLPFSQWSKSFADDVTLTAAMLDRLLHHCHVVQISGESYRLKDRKRIGIQPQVET
AENVVFLGASGVGKTHLSMALGYKAIMNNIKIKFITAADLMLQLSTAYQQGKLKTYMQRAVLGPKLLIIDEIGYLPFGREEANLFFNVIAKRYEKGSTIL
TSNLPFSQWSKSFADDVTLTAAMLDRLLHHCHVVQISGESYRLKDRKRIGIQPQVET
Blast result :
Comments
ISAba8 is 72%(ORF1) aa similar to ISPsy4 and 79%(ORF2) to ISRso19.
References
1] Laurent Poirel (20006) Direct submission.