ISAba8

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter baumannii
Acinetobacter baumannii
DNA section
IS Length : 1955 bp

Ends


IR Length : 27

IRL : TGTTAGCGGCAGTGTAAAAATGACCCCCTAATGGCATTTAAAAACTGACC
IRR : TGTCAACGGCATTCTAAAATTGACCCCTTTTACCTTACAAATGGCAAAGT

Insertion site


Left flankDirect repeatRight flankDR Length
AATATCGAATCCATGAACAGTGGT4

DNA sequence

TGTTAGCGGCAGTGTAAAAATGACCCCCTAATGGCATTTAAAAACTGACCCCCTTGGTTAATATAGCGGTCATTTTGGACTGCTCAACATGTTAACTTTG
GAGCAAGCAGTGACAATCCAAATACTTCATCAGCAAGGTAAATCTATTAAAGCTATTACTCGTGAACTGGGGGTGTCCAGAAATACCGTACGTAAATATT
TACGGCAAAACACCACACCTCAGTATCAGCGTATACAGCCTAGAATAAGTATCCTTGACCCATATAAACCCTATTTACTCCAACGTGTAAACGCAGCTCA
TCCTGAATGGATTCCTGCTGTTGTGCTCTACCAGGAAATTTTAGGGTTGGGATATCCAGGAAAGATCAGGATCTTGAGGGAATATCTTGCAACATTAAAA
CCAGTTGCCAAACCGGAACCGATCATTCGTTTTGAAACCCAACCTGGCCAACAAATGCAGGTGGATTTCACTACGATTCGACGCAACAACACGACTTTAA
AAGCCTTTGTCGCAACATTAGGGTATTCCAGAGCGACTTTCGTGAAATTTTATGATCATGAACGTACGGATGCATGGATCGATGGTCTAGAAAATGCATT
TCAGTTCTTTGCAGGAGTACCTCAAGAAATCCTGTTTGATAACGCTAAAACGATCATGATTGAACGGGATGCCTATCAGGAGGGGCAGCATAAATGGAAC
CCCAAACTGCTCGACTGCGCCAAGAAGTACAGCTTTCGTCCTCGCGTATGTAAGCCCTACCGTGCACAGACCAAAGGCAAAGTTGAACGCTTTAATGGAT
ACCTCAAATCAAGCTTTATTGTGCCATTGAAAGCAAGCCTGAAGACTTCGGGTTTACTGTTAGATGTTGATGTCGCCAATGCCCACATTGGCCGGTGGCT
GCATGAAACCGCCAATCAGCGGATTCATGCCACCACGCAAGAAAAACCGGCTGTTCGACTGCAACAGGAGCAGCAAAAATTTACGCCATTACCGCAATCA
GATACAGGTTCTAGCACTGTACCTGTTGCAGCAGCACAGCATGTCATGCCCTACGAGAGTTTGCAGCATCCCTTATCGGTATATGACCAGTTGCTGGGAG
TTTCCTGATGAATCTGCAATACGACCGTATAGAGCAGCTTTGCCAAAAGCTCAATCTGCCTGCCATCGCATCACAATGGTCACATCATGCACAACAAACA
TTAGGTCAAGATGGAAGTTATGCCGACTTTGTTGAAGCGATCTTGACGCATGAATATGCTGCCAGGCAACAGCGCAGTCAGCAGGTACTGATGAAACTCT
CAGGCCTGCCTCAAGTCAAAACCCTGGAAGACTATGATTTTAATTATGCCCTAGGGGCCCCTAAATCACAGGTGATTGAACTGTTCAATCTGAGCTTTAT
TGCTAGAGCAGAAAATGTGGTGTTTCTCGGGGCTAGCGGAGTAGGAAAAACGCACTTATCTATGGCATTAGGCTATAAGGCCATCATGAACAACATTAAG
ATCAAGTTCATTACCGCAGCGGATTTAATGCTGCAACTGAGTACAGCCTATCAGCAAGGTAAATTGAAAACCTATATGCAGCGTGCGGTACTGGGACCCA
AGTTACTGATTATCGATGAAATTGGTTATTTACCGTTTGGACGTGAAGAAGCGAATCTGTTCTTTAACGTGATTGCCAAGCGTTATGAAAAAGGCAGTAC
GATATTGACGAGTAACTTACCCTTTAGCCAGTGGTCCAAATCCTTTGCAGATGATGTCACGTTGACCGCTGCGATGTTAGATCGGCTATTACATCACTGT
CATGTGGTACAAATATCGGGTGAGAGTTATCGTTTGAAGGATAGAAAAAGAATTGGGATTCAACCCCAGGTTGAAACTTAATACGAGATCAAAGGGGTCA
ATTTTACTTTGCCATTTGTAAGGTAAAAGGGGTCAATTTTAGAATGCCGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1020 bp339 aa891108+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLTLEQAVTIQILHQQGKSIKAITRELGVSRNTVRKYLRQNTTPQYQRIQPRISILDPYKPYLLQRVNAAHPEWIPAVVLYQEILGLGYPGKIRILREYL
ATLKPVAKPEPIIRFETQPGQQMQVDFTTIRRNNTTLKAFVATLGYSRATFVKFYDHERTDAWIDGLENAFQFFAGVPQEILFDNAKTIMIERDAYQEGQ
HKWNPKLLDCAKKYSFRPRVCKPYRAQTKGKVERFNGYLKSSFIVPLKASLKTSGLLLDVDVANAHIGRWLHETANQRIHATTQEKPAVRLQQEQQKFTP
LPQSDTGSSTVPVAAAQHVMPYESLQHPLSVYDQLLGVS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
774 bp257 aa11081881+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNLQYDRIEQLCQKLNLPAIASQWSHHAQQTLGQDGSYADFVEAILTHEYAARQQRSQQVLMKLSGLPQVKTLEDYDFNYALGAPKSQVIELFNLSFIAR
AENVVFLGASGVGKTHLSMALGYKAIMNNIKIKFITAADLMLQLSTAYQQGKLKTYMQRAVLGPKLLIIDEIGYLPFGREEANLFFNVIAKRYEKGSTIL
TSNLPFSQWSKSFADDVTLTAAMLDRLLHHCHVVQISGESYRLKDRKRIGIQPQVET

 

Blast result :
Comments
ISAba8 is 72%(ORF1) aa similar to ISPsy4 and 79%(ORF2) to ISRso19.
References
1] Laurent Poirel (20006) Direct submission.