ISVvu3
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005140 | ND | Vibrio vulnificus | Vibrio vulnificus YJ016 |
DNA section
IS Length : 1430 bp
Ends
IR Length : 15/18
IRL : CAATGTAGTTCACTTAAGAGGAGCTGCCTTGCGATTAACAGGATATCGGG
IRR : CAATGTAGATCAGATAAGGATCGGTTGACCGATCCTTCTGATGAGAGACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTAAAAGGGT | CGCCATGTGCG | ACCCTTTTCA | 11 |
| AAAAAACCCC | CAATCAATTGG | GGGCTTCTTA | 11 |
| ACAGAAAGGC | TGCCTTTGGCG | GCCTCTTCTA | 11 |
| ATAAAAACGC | CGCTGTATAGCG | GCGTTTTGTG | 12 |
| AATCAAAGCC | CGCTTACCAGCG | GGCTTTTTAT | 12 |
| AAAAAACACC | CACTGCGACGTG | GGTGTTTTTA | 12 |
| AGCAAAAACT | CAATGAAAAAAGG | CGCATTTTGC | 13 |
| AAGAAAGCCT | TGGCATTTCACCA | AGGCTTTTAT | 13 |
DNA sequence
CAATGTAGTTCACTTAAGAGGAGCTGCCTTGCGATTAACAGGATATCGGGTCTTTGATATTTTTACCGCCCTAGGCCGATTTGGCTTAGGGCGTTTGTCT
ATAAAAAGGATGGATAAGTCTCCGCGCAGACTTTTTAGACGCTTCGGTGTATTACCTGGAGATACCGCTTTGCTCATTACTTTCAGTTGACTAGCGATGA
ACTGACAAGCGTATTTAAAACTGATTTCATTGGCCATTCGACCATGTGCTACGGCCGCTTGACTGGCTTCTCGTCTTACCAAGTTATAACCAAGCAACAG
CCCCCATAACTCTTGATAGACAAGCTCGACCGTCTTACTCCTCAGCACTAACGCGTTGTGCTGCATCGAACTTTTTATGTCTCTGTACCCTAACTCAATT
TCCCATCTCTCATGGTAAAGCTCGGCCACTGATTCCGCATCGTACTCTTCCCTAGGCAAAGACGTAAACACTGTTTTGTGCTTACCTTGTACTTGATAAG
TAACGGCTCTGACTTGCCATTTTTCAGGTAGGTTAGGATTTTTCTTGCGAGCCTGTGGAGAGACTTTCATTTCTACCAACATGTCGTCGCTCTCTTCATC
GTCCAGAAGTCTGAATTTCACCCCTTTCTTCGCTGGTAACAACCAATGCCTGTTGGAGCCACTATTTTGGAGAGAAAGAAGTAAGTCTGCGCCATAAAAC
CCCTTATCTAGTAAAGTCACTGAGTTATCAGGTAGAGAGTCAATAAAGGGCATTGCAAGCGGTATTTCACCACGACGATAGGGGCTTATAGCTGCGTCTA
CAATGACATGTGAGCGAACATTCATCATGGTCACCACCCTGAGTACTGGATGAGGTGTCTGCCTCTCGCTGGATGTATTACCTGACCCAAAATGCTCTCT
TAGTTCTGATGTATCAGCGGTTCGAAAAAGAGCCCCATCAATAGCGAAGACTTGTAAGCCTTGCCAAGTATCCTCGGGGTAACGCTCTAGACCCCATGTA
TGACTGCATTGTCTAAACAACCATTCTGGAGCAGCTTTACCAAGGCGTTGCCTTGCTTGCGTTAAAGCACTTTTAGCTAGCAGTTCCTCATCGGCTAAGC
CCTCGGCACAGACATTCATTCGCCGTGCGACTTCTGCAATAGACTCATTGCGGAAAAACGCCATTCCAACAATAAGCCAAAGAACCATATCACTAGGTAA
ACGACGTCGACGAATTGTCGCTTTATCGGAAAGTGTTGCCGCTTTAGCAACCCACTCATCAGGAATATGCTCAGAGAAAGTGGTGAGCTGAGCGACATCA
ACAGGACTCTCTTCAAGAAAGTCAGCAAAATAGTTTTGGATAGACATAAAAAATCGGAAACCTATAAACAGGTTTCCGATTGTCTCTCATCAGAAGGATC
GGTCAACCGATCCTTATCTGATCTACATTG
ATAAAAAGGATGGATAAGTCTCCGCGCAGACTTTTTAGACGCTTCGGTGTATTACCTGGAGATACCGCTTTGCTCATTACTTTCAGTTGACTAGCGATGA
ACTGACAAGCGTATTTAAAACTGATTTCATTGGCCATTCGACCATGTGCTACGGCCGCTTGACTGGCTTCTCGTCTTACCAAGTTATAACCAAGCAACAG
CCCCCATAACTCTTGATAGACAAGCTCGACCGTCTTACTCCTCAGCACTAACGCGTTGTGCTGCATCGAACTTTTTATGTCTCTGTACCCTAACTCAATT
TCCCATCTCTCATGGTAAAGCTCGGCCACTGATTCCGCATCGTACTCTTCCCTAGGCAAAGACGTAAACACTGTTTTGTGCTTACCTTGTACTTGATAAG
TAACGGCTCTGACTTGCCATTTTTCAGGTAGGTTAGGATTTTTCTTGCGAGCCTGTGGAGAGACTTTCATTTCTACCAACATGTCGTCGCTCTCTTCATC
GTCCAGAAGTCTGAATTTCACCCCTTTCTTCGCTGGTAACAACCAATGCCTGTTGGAGCCACTATTTTGGAGAGAAAGAAGTAAGTCTGCGCCATAAAAC
CCCTTATCTAGTAAAGTCACTGAGTTATCAGGTAGAGAGTCAATAAAGGGCATTGCAAGCGGTATTTCACCACGACGATAGGGGCTTATAGCTGCGTCTA
CAATGACATGTGAGCGAACATTCATCATGGTCACCACCCTGAGTACTGGATGAGGTGTCTGCCTCTCGCTGGATGTATTACCTGACCCAAAATGCTCTCT
TAGTTCTGATGTATCAGCGGTTCGAAAAAGAGCCCCATCAATAGCGAAGACTTGTAAGCCTTGCCAAGTATCCTCGGGGTAACGCTCTAGACCCCATGTA
TGACTGCATTGTCTAAACAACCATTCTGGAGCAGCTTTACCAAGGCGTTGCCTTGCTTGCGTTAAAGCACTTTTAGCTAGCAGTTCCTCATCGGCTAAGC
CCTCGGCACAGACATTCATTCGCCGTGCGACTTCTGCAATAGACTCATTGCGGAAAAACGCCATTCCAACAATAAGCCAAAGAACCATATCACTAGGTAA
ACGACGTCGACGAATTGTCGCTTTATCGGAAAGTGTTGCCGCTTTAGCAACCCACTCATCAGGAATATGCTCAGAGAAAGTGGTGAGCTGAGCGACATCA
ACAGGACTCTCTTCAAGAAAGTCAGCAAAATAGTTTTGGATAGACATAAAAAATCGGAAACCTATAAACAGGTTTCCGATTGTCTCTCATCAGAAGGATC
GGTCAACCGATCCTTATCTGATCTACATTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1335 bp | 445 aa | 84 | 1418 | + | No |
Chemistry : DDE
ORF sequence :
MSIQNYFADFLEESPVDVAQLTTFSEHIPDEWVAKAATLSDKATIRRRRLPSDMVLWLIVGMAFFRNESIAEVARRMNVCAEGLADEELLAKSALTQARQ
RLGKAAPEWLFRQCSHTWGLERYPEDTWQGLQVFAIDGALFRTADTSELREHFGSGNTSSERQTPHPVLRVVTMMNVRSHVIVDAAISPYRRGEIPLAMP
FIDSLPDNSVTLLDKGFYGADLLLSLQNSGSNRHWLLPAKKGVKFRLLDDEESDDMLVEMKVSPQARKKNPNLPEKWQVRAVTYQVQGKHKTVFTSLPRE
EYDAESVAELYHERWEIELGYRDIKSSMQHNALVLRSKTVELVYQELWGLLLGYNLVRREASQAAVAHGRMANEISFKYACQFIASQLKVMSKAVSPGNT
PKRLKSLRGDLSILFIDKRPKPNRPRAVKISKTRYPVNRKAAPLK
RLGKAAPEWLFRQCSHTWGLERYPEDTWQGLQVFAIDGALFRTADTSELREHFGSGNTSSERQTPHPVLRVVTMMNVRSHVIVDAAISPYRRGEIPLAMP
FIDSLPDNSVTLLDKGFYGADLLLSLQNSGSNRHWLLPAKKGVKFRLLDDEESDDMLVEMKVSPQARKKNPNLPEKWQVRAVTYQVQGKHKTVFTSLPRE
EYDAESVAELYHERWEIELGYRDIKSSMQHNALVLRSKTVELVYQELWGLLLGYNLVRREASQAAVAHGRMANEISFKYACQFIASQLKVMSKAVSPGNT
PKRLKSLRGDLSILFIDKRPKPNRPRAVKISKTRYPVNRKAAPLK
Blast result :
Comments
ISVvu3 is 54% aa similar to IS4.
ISVvu3 has been found by screening completely sequenced bacterial genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-76-D(N3)-103-E(C1). The copy number in Vibrio vulnificus YJ016 is 1 on chromosome II and 7 on chromosome I.
ISVvu3 has been found by screening completely sequenced bacterial genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-76-D(N3)-103-E(C1). The copy number in Vibrio vulnificus YJ016 is 1 on chromosome II and 7 on chromosome I.
References
1] Chen,C.Y., Wu,K.M., Chang,Y.C., Chang,C.H., Tsai,H.C., Liao,T.L., Liu,Y.M., Chen,H.J., Shen,A.B., Li,J.C., Su,T.L., Shao,C.P., Lee,C.T., Hor,L.I. and Tsai,S.F. (2003) Genome Res. 13,2577-2587