ISNpu5
- Family IS701
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Nostoc punctiforme | Nostoc punctiforme PCC 73102 |
DNA section
IS Length : 1397 bp
Ends
IR Length : 18/19
IRL : TTAACCCTCTTGTGTCACTATCGCGGTAGTATAATAATGTACAAAGCCTA
IRR : TTAACCCTGTTGTGTCACTTTAGGCAGAGGAAAAATAGAAATCAGGGTCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACCGTTAAC | TCTGATTTTT | 0 |
DNA sequence
TTAACCCTCTTGTGTCACTATCGCGGTAGTATAATAATGTACAAAGCCTAGAACCAAGACATAAAGCATGGTAGAGCCTCGTTCACCCATAAAAACCGTC
AAGTTTGTGGATGAATATTGCTTATGGTATAAAAACCTGTTTTCAGATGTTAGGAATTTTGAAGCTTTCAAATATCTACATATAGGATGTATTTCCGATT
TAAAACGGAAAAGTCTACCTGAAATAGCGAAAATTGTCGGGCTAGATAACTATCAAGGACTACATCATTTCTTAACTACACCATCATGGTCAGTAGAACA
GTTAAGAGCTTTGCGATTACAACTAATTTTAGAGGTACTAAAAGGAAGACCAATCATTTTAATTATTGATGAAACAGGAGATAAAAAGAAAGGTAATACC
ACAGATTATGTGAAACGTCAGTACATAGGCAATTTAGGAAAAGTAGAGAATGGCGTTGTGGCAGTCACAGCTTACGGTATGTTCTGTGGAATGACATTTC
CACTGCTGTTTGAAGTATACAAGCCACGAGAAAAATTAAAGCCAGGAGACAAATATCTTACTAAGCCTCAAATCGGGGCAATGCTAATACGCAAGCTACA
GTTAATGGGATTCAAATTCAACTTGGTACTAGCAGATAGCTTATATGGTGAGAGTAGCACAAATTTTATACCCGTATTAGATGAATTAGATTTAAATTAT
CTAGTAGCAATTCGCTCAAACCATTCTGTAGCATTGCTTAAAGGTCAGTATACTCAATATTTAAACTGGCATAAGTTTAAAAGAGTATTCTCTGACTTAA
GTAGTGAGAATCGGTTTATTAGAGAAATAATACATGGCAAACGTAGCAATCATAGGTATTGGCAAATTACTACAGACACTCTTAACTTACCTGGAAACTC
TACCTGGTATGTAATGAGTAAATACCCAGACATTACACCAAGAGAAGTTGGGAATTTCTATGGCTTTAGGACATGGGTTGAGTATGGTTTAAAACAAAGT
AAGAATCAGTTAGGTTGGGCAGATTATCGTTTTACTTGCTATGAAGATATTGAACGGTGGTGGGAGATTATTTGTAGCGCCTACTTAATGGTTAGTCTTC
ATTCAGAATCTCTGCGCCCTTATCCCCTAGATTCTCAATCAACATTTGCTTCACATCAGAGGTGGGATAATGGTAAAGGTTGGAAGAATATTCTCAATAA
TCTCCGTCTCATCCTTCAACCTTTTACCCTATTTAATTTAATTCAGCCTTGGCTTTCAGTTTTTCCTATTCCTCATTTATCTTTGGGATTTGCTAAACTC
CAATCTGTTGTTTATTACCTTACCTATCCTACTTTTATATCCCTGACTGACCCTGATTTCTATTTTTCCTCTGCCTAAAGTGACACAACAGGGTTAA
AAGTTTGTGGATGAATATTGCTTATGGTATAAAAACCTGTTTTCAGATGTTAGGAATTTTGAAGCTTTCAAATATCTACATATAGGATGTATTTCCGATT
TAAAACGGAAAAGTCTACCTGAAATAGCGAAAATTGTCGGGCTAGATAACTATCAAGGACTACATCATTTCTTAACTACACCATCATGGTCAGTAGAACA
GTTAAGAGCTTTGCGATTACAACTAATTTTAGAGGTACTAAAAGGAAGACCAATCATTTTAATTATTGATGAAACAGGAGATAAAAAGAAAGGTAATACC
ACAGATTATGTGAAACGTCAGTACATAGGCAATTTAGGAAAAGTAGAGAATGGCGTTGTGGCAGTCACAGCTTACGGTATGTTCTGTGGAATGACATTTC
CACTGCTGTTTGAAGTATACAAGCCACGAGAAAAATTAAAGCCAGGAGACAAATATCTTACTAAGCCTCAAATCGGGGCAATGCTAATACGCAAGCTACA
GTTAATGGGATTCAAATTCAACTTGGTACTAGCAGATAGCTTATATGGTGAGAGTAGCACAAATTTTATACCCGTATTAGATGAATTAGATTTAAATTAT
CTAGTAGCAATTCGCTCAAACCATTCTGTAGCATTGCTTAAAGGTCAGTATACTCAATATTTAAACTGGCATAAGTTTAAAAGAGTATTCTCTGACTTAA
GTAGTGAGAATCGGTTTATTAGAGAAATAATACATGGCAAACGTAGCAATCATAGGTATTGGCAAATTACTACAGACACTCTTAACTTACCTGGAAACTC
TACCTGGTATGTAATGAGTAAATACCCAGACATTACACCAAGAGAAGTTGGGAATTTCTATGGCTTTAGGACATGGGTTGAGTATGGTTTAAAACAAAGT
AAGAATCAGTTAGGTTGGGCAGATTATCGTTTTACTTGCTATGAAGATATTGAACGGTGGTGGGAGATTATTTGTAGCGCCTACTTAATGGTTAGTCTTC
ATTCAGAATCTCTGCGCCCTTATCCCCTAGATTCTCAATCAACATTTGCTTCACATCAGAGGTGGGATAATGGTAAAGGTTGGAAGAATATTCTCAATAA
TCTCCGTCTCATCCTTCAACCTTTTACCCTATTTAATTTAATTCAGCCTTGGCTTTCAGTTTTTCCTATTCCTCATTTATCTTTGGGATTTGCTAAACTC
CAATCTGTTGTTTATTACCTTACCTATCCTACTTTTATATCCCTGACTGACCCTGATTTCTATTTTTCCTCTGCCTAAAGTGACACAACAGGGTTAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1308 bp | 436 aa | 68 | 1375 | + | No |
Chemistry : DDE
ORF sequence :
MVEPRSPIKTVKFVDEYCLWYKNLFSDVRNFEAFKYLHIGCISDLKRKSLPEIAKIVGLDNYQGLHHFLTTPSWSVEQLRALRLQLILEVLKGRPIILII
DETGDKKKGNTTDYVKRQYIGNLGKVENGVVAVTAYGMFCGMTFPLLFEVYKPREKLKPGDKYLTKPQIGAMLIRKLQLMGFKFNLVLADSLYGESSTNF
IPVLDELDLNYLVAIRSNHSVALLKGQYTQYLNWHKFKRVFSDLSSENRFIREIIHGKRSNHRYWQITTDTLNLPGNSTWYVMSKYPDITPREVGNFYGF
RTWVEYGLKQSKNQLGWADYRFTCYEDIERWWEIICSAYLMVSLHSESLRPYPLDSQSTFASHQRWDNGKGWKNILNNLRLILQPFTLFNLIQPWLSVFP
IPHLSLGFAKLQSVVYYLTYPTFISLTDPDFYFSSA
DETGDKKKGNTTDYVKRQYIGNLGKVENGVVAVTAYGMFCGMTFPLLFEVYKPREKLKPGDKYLTKPQIGAMLIRKLQLMGFKFNLVLADSLYGESSTNF
IPVLDELDLNYLVAIRSNHSVALLKGQYTQYLNWHKFKRVFSDLSSENRFIREIIHGKRSNHRYWQITTDTLNLPGNSTWYVMSKYPDITPREVGNFYGF
RTWVEYGLKQSKNQLGWADYRFTCYEDIERWWEIICSAYLMVSLHSESLRPYPLDSQSTFASHQRWDNGKGWKNILNNLRLILQPFTLFNLIQPWLSVFP
IPHLSLGFAKLQSVVYYLTYPTFISLTDPDFYFSSA
Blast result :
Comments
ISNpu5 is 87% aa similar to IS701. ISNpu5 was found by screening completely sequenced genomes for sequences homologous to the IS701 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-88-D(N3)-114-E(C1). The chromosome sequencing of Nostoc punctiforme PCC 73102 is currently in progress. Accesion number: NZ_AAAY00000000.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] NCBI Microbial Genomes Annotation Project (2006) Unpublished
2] NCBI Microbial Genomes Annotation Project (2006) Unpublished