ISLhe10

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISLhe15
Accession numberTranspositionOriginHost
NC_010080 ND Lactobacillus helveticus
Lactobacillus kefiranofaciens ZW3
Lactobacillus helveticus DPC 4571
Lactobacillus helveticus R0052
Lactobacillus helveticus H10
DNA section
IS Length : 1598 bp

Ends


IR Length : 19/24

IRL : CTAAGTGTATAATTTTTTGCGGTAAGTTGAATAAATAATTAAAAAAGCTC
IRR : GTAAGTGTCAATATTTTTGCGGTAGGCTATTAACCATTAGTCTGACCTTA

Insertion site


Left flankDirect repeatRight flankDR Length
ATTGTTAAATTAAAGTAGGCAATCTTCTTGT9
TGAGCGGATTACTGATACGATCAGGTCTTAG9
CAGTTAAGATTATCTATCATCAATTTTTTGC9
CTTTAAAATGAAATAAGATCCCCGAACTTTT9
TATTTATTATAATATTAATCAAAAATATTTT9
AAATAAAAATCGATCTAAACATATTTATATA9
AGACACTTTTTCTGGTTAAATATTAACTTGC9
AATATCCCAGCTAAGTAGTGTATAATATTGA9
CTTCATAACCTAACTGTTGCACTTAAATTGA9
CGGATGATGCAGATATTAAAAAATATGTTGA9
AAAAAATAATTTTTTTGAAAAATATGCTTGC9
AATTTGAATACAAGTAGATCTCTTTATTTGA9
ATTATTATCGTAAAAAACGT0

DNA sequence

CTAAGTGTATAATTTTTTGCGGTAAGTTGAATAAATAATTAAAAAAGCTCAAATCCTTTGAAATTGGACTTGAGCTCAAACAAAAAGACCAGTAGTCTGA
TGTTAACGAAAAAAACACATTAGAAGCTGGTCTTTTATGGAATCTATTATAGCTGATATCGTGAAAATTATTAAGTCTGAAAATAATGTTATTGCCCGTG
AAAAGGCATTAATGTGCTACTTCTTTGATCTGATTAGAGAATTGATGACAGCAGCACTAGAAGAAGTTGATGCTGGTCTAGTTGAAGAGACTAAAAAGCA
AGGCTATCAGATTGAAAAGAAAAATAAGCGCTCAGTTGTGACTGCCTTTGGTGAGATTAGCTATTGGCGCAGAAGATATGTCTGTCCAGGCAAGAAGGCC
CAATACCCACTGGATAAGCTGATGGGCTATGACAAATACAAGCGTTACAGTGTTTTGGCAGTAAAAGATGTTTTGCAGGTTAGCGCCGTGGCAACTTACC
GCAATACCGCTCTAGCAGTTAATACCCTTTCTTGTTTTAAGATTAGTCACAGCCAAGTTGGCAAGCTGATTGTGCAAGCAGGCAAACAGATTAAAGCACA
GCAGCAGTCTGAGGAAAGATACGATGGCATTACCCAAAAGAAAAAGGTGCCAGTGCTTTATCTTGAAGGCGATGGCGTTGTGATCAAGGGCACCAAGAAG
CGACTAGAGTTTCATCGCTATCAGGTCTGCGAGGATATTATTAATTTAAGCAAGACGCGCAGAAAGAGAGTACAGGCCAAAGAGTTTGTTTCTTTAAGCC
GCTTGGATGCTTTGCAAGAAATTAAAGCTTATTTAGCCAATGCCTATGACTTAAGCGATACTTTAATCATCAGTAATGCCGATGGCGGTGCTGGCTATGC
CAAGAAAGATTTTGATGAAATAGTTGGCTATTGCCGGCAGCACGAACACTTCTTGGATGTCTTTCACTTGAATAAAAAGATCAAGGACCGTTTAGGCTTT
ATGCCGGCTATGCAAAGCAAATTGATCAGTGCAGTTGAGTTTAAATATGACCGTCACTTAACTGATGTAATTTTAGACACGATTGAAAGCAATCTGGTTG
ATGAGCTGAATACGCCAGAAAATCATGAGAATTTAAGGCGCCTGCGCAGCTATCTTCATCGTCGCTGGGTGGATATTAAGCCATTCAAAATGCGTCATTT
GTCCGTAATTAAAGCAATTGGCTGCTGTGAGAGCAATCACCGCAAGTATACTTATCGCGTCAAAGGGCAAGGAAAATACTGGTCAGAAGATGGTGCCGAA
GGAATTCTGCGTGTACTGACTTGTATCAAAAACAATGAGCTGGAATACTGGCTTAGCAGTGAGTTTGCAGGTGGACAGCTCGATATTGGTGATCAGGAAG
ATTTGAAAGGTGCAGTTCGTGCCAGCCTAAGAAAGACGCATGGAGTTCATCTGGGCATTCATCACGGCGCCATTGAAAGCTTAACAGCAGGTCATAGCTA
TTTGGATGATTTTAGCAGAAAAATAAATCAAATAAATATTTAAGAAATTAAGGTCAGACTAATGGTTAATAGCCTACCGCAAAAATATTGACACTTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1407 bp468 aa1371543+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MESIIADIVKIIKSENNVIAREKALMCYFFDLIRELMTAALEEVDAGLVEETKKQGYQIEKKNKRSVVTAFGEISYWRRRYVCPGKKAQYPLDKLMGYDK
YKRYSVLAVKDVLQVSAVATYRNTALAVNTLSCFKISHSQVGKLIVQAGKQIKAQQQSEERYDGITQKKKVPVLYLEGDGVVIKGTKKRLEFHRYQVCED
IINLSKTRRKRVQAKEFVSLSRLDALQEIKAYLANAYDLSDTLIISNADGGAGYAKKDFDEIVGYCRQHEHFLDVFHLNKKIKDRLGFMPAMQSKLISAV
EFKYDRHLTDVILDTIESNLVDELNTPENHENLRRLRSYLHRRWVDIKPFKMRHLSVIKAIGCCESNHRKYTYRVKGQGKYWSEDGAEGILRVLTCIKNN
ELEYWLSSEFAGGQLDIGDQEDLKGAVRASLRKTHGVHLGIHHGAIESLTAGHSYLDDFSRKINQINI

 

Blast result :
Comments
ISLhe10 is 53% aa similar to ISLca4.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
ISLhe15 is an isoform of ISLhe10, the transposase is mutated with unexpected frameshift.
References
1] Romain Guérillot and Philippe Glaser (2012) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted);
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).