ISNisp3
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAMY01000048 | ND | Nitrobacter sp. | Nitrobacter sp. Nb-311A |
DNA section
IS Length : 1234 bp
Ends
IR Length : 25/43
IRL : TGATCTGCGACCTTCAAATCGGACCGTTTTTAGTTAGAGTTTTTCCGCTG
IRR : TGATCCGAGACCATTCTTTGGACCGCCGGAGGCTAGAGTTTCCGGCTTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAGGCCAAA | ACGA | AAACCAGGAC | 4 |
DNA sequence
TGATCTGCGACCTTCAAATCGGACCGTTTTTAGTTAGAGTTTTTCCGCTGGATTTCCTCGGACTGGGAGGAGCGGAGAGCGATGAAAGCCTCGAAGTTTT
CAGACGCGCAGAAGGCATTTATTCTGAAGCAGGGTTCCGATGGGGTGCCGGTGGCCGATATTTGCCGTAAGGCCGGGATCAGCCAGGCGACCTTTTTCAA
CTGGAGAAGGAAGTACGACGGCTTGCTGCCGACCGAGATGCGGCGGCTGAAGCAGCTTGAGGATGAGAATGGCAAGCTGAAAAAACTGGTCGCTGATCTC
AGCCTGGATCGCGAGATGTTGCAGGACGTCATCCGCCGAAAAATCTGAGGCCTGTTCGTCAGCGCAAGCTCGTCGACGCGGTCCGCGGTGAATGGGATGT
CTCGATCCGGCGGGTGTGCCAGATACTCGAGGTCGACAGATCAACCTATCACTACAAGTCGCGCCGGCCCGGACAGGCCAGCCTCGAAAGCAAGATCAAG
GAGATCTGCCAGACGCGGGTTCGCTACGGCTACCGCCGCGTGCATGTGCTGCTGCGCCGCGAAGGCTGGCTAATCAACCAGAAGAAGACGCGACGGATTT
ATCGCGAGTTAGGCCTGCAATTGCGCAACAAAACGCCGAAGCGACGGGTCAGGGCGAAGCTGCGCGACGATCGCAGAGATGCCACGCATCCCAACGAGAC
CTGGGCGATGGACTTTGTGCACGATCAGCTCGCCACGGGAAACAAGATTCGTGTGCTCACCGTTGTCGACTTATTCTCACGGTACTCGCCGGTGCTTGAT
CCGCGCTTCAGCTACCGCGCCGAGAATGTCGTTCAAACGCTGGAGCACACCTGGGCAGCCGTTGGTTATCCGAAGACCATCCGCGTCGATCAAGGCTCCG
AGTTCATCTCGCGCGAACTGGATCTGTGGGCCTATTCCAATGGTGTCACGCTCGACTTCAGTCGGCCTGGCAAACCGACTGACAATGCGTTCATCGAAGC
CTTCAATGGACGCTTCCGCGCGGAGTGTCTGAACACTCATTGGTTCCTGACCCTTGACGACGCACGATCAAAATTGGAAGATTGGCGCAGATACTACAAC
GAACAACGCCCGCATGGGGCGATCGGGAATAGACCCCCGATTACGTTGCATAATCGTGACAGCGATCCCAGCTCGCCGTCATGACAAAGCCGGAAACTCT
AGCCTCCGGCGGTCCAAAGAATGGTCTCGGATCA
CAGACGCGCAGAAGGCATTTATTCTGAAGCAGGGTTCCGATGGGGTGCCGGTGGCCGATATTTGCCGTAAGGCCGGGATCAGCCAGGCGACCTTTTTCAA
CTGGAGAAGGAAGTACGACGGCTTGCTGCCGACCGAGATGCGGCGGCTGAAGCAGCTTGAGGATGAGAATGGCAAGCTGAAAAAACTGGTCGCTGATCTC
AGCCTGGATCGCGAGATGTTGCAGGACGTCATCCGCCGAAAAATCTGAGGCCTGTTCGTCAGCGCAAGCTCGTCGACGCGGTCCGCGGTGAATGGGATGT
CTCGATCCGGCGGGTGTGCCAGATACTCGAGGTCGACAGATCAACCTATCACTACAAGTCGCGCCGGCCCGGACAGGCCAGCCTCGAAAGCAAGATCAAG
GAGATCTGCCAGACGCGGGTTCGCTACGGCTACCGCCGCGTGCATGTGCTGCTGCGCCGCGAAGGCTGGCTAATCAACCAGAAGAAGACGCGACGGATTT
ATCGCGAGTTAGGCCTGCAATTGCGCAACAAAACGCCGAAGCGACGGGTCAGGGCGAAGCTGCGCGACGATCGCAGAGATGCCACGCATCCCAACGAGAC
CTGGGCGATGGACTTTGTGCACGATCAGCTCGCCACGGGAAACAAGATTCGTGTGCTCACCGTTGTCGACTTATTCTCACGGTACTCGCCGGTGCTTGAT
CCGCGCTTCAGCTACCGCGCCGAGAATGTCGTTCAAACGCTGGAGCACACCTGGGCAGCCGTTGGTTATCCGAAGACCATCCGCGTCGATCAAGGCTCCG
AGTTCATCTCGCGCGAACTGGATCTGTGGGCCTATTCCAATGGTGTCACGCTCGACTTCAGTCGGCCTGGCAAACCGACTGACAATGCGTTCATCGAAGC
CTTCAATGGACGCTTCCGCGCGGAGTGTCTGAACACTCATTGGTTCCTGACCCTTGACGACGCACGATCAAAATTGGAAGATTGGCGCAGATACTACAAC
GAACAACGCCCGCATGGGGCGATCGGGAATAGACCCCCGATTACGTTGCATAATCGTGACAGCGATCCCAGCTCGCCGTCATGACAAAGCCGGAAACTCT
AGCCTCCGGCGGTCCAAAGAATGGTCTCGGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 82 | 348 | + | No |
Description : First part of the transposase
ORF sequence :
MKASKFSDAQKAFILKQGSDGVPVADICRKAGISQATFFNWRRKYDGLLPTEMRRLKQLEDENGKLKKLVADLSLDREMLQDVIRRKI
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 888 bp | 295 aa | 297 | 1184 | + | No |
Description : Second part of the transposase
ORF sequence :
SQPGSRDVAGRHPPKNLRPVRQRKLVDAVRGEWDVSIRRVCQILEVDRSTYHYKSRRPGQASLESKIKEICQTRVRYGYRRVHVLLRREGWLINQKKTRR
IYRELGLQLRNKTPKRRVRAKLRDDRRDATHPNETWAMDFVHDQLATGNKIRVLTVVDLFSRYSPVLDPRFSYRAENVVQTLEHTWAAVGYPKTIRVDQG
SEFISRELDLWAYSNGVTLDFSRPGKPTDNAFIEAFNGRFRAECLNTHWFLTLDDARSKLEDWRRYYNEQRPHGAIGNRPPITLHNRDSDPSSPS
IYRELGLQLRNKTPKRRVRAKLRDDRRDATHPNETWAMDFVHDQLATGNKIRVLTVVDLFSRYSPVLDPRFSYRAENVVQTLEHTWAAVGYPKTIRVDQG
SEFISRELDLWAYSNGVTLDFSRPGKPTDNAFIEAFNGRFRAECLNTHWFLTLDDARSKLEDWRRYYNEQRPHGAIGNRPPITLHNRDSDPSSPS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1103 bp | 367 aa | 82 | 1184 | + | Yes |
Chemistry : DDE
ORF sequence :
MKASKFSDAQKAFILKQGSDGVPVADICRKAGISQATFFNWRRKYDGLLPTEMRRLKQLEDENGKLKKLVADLSLDREMLQDVIRRKNLRPVRQRKLVDA
VRGEWDVSIRRVCQILEVDRSTYHYKSRRPGQASLESKIKEICQTRVRYGYRRVHVLLRREGWLINQKKTRRIYRELGLQLRNKTPKRRVRAKLRDDRRD
ATHPNETWAMDFVHDQLATGNKIRVLTVVDLFSRYSPVLDPRFSYRAENVVQTLEHTWAAVGYPKTIRVDQGSEFISRELDLWAYSNGVTLDFSRPGKPT
DNAFIEAFNGRFRAECLNTHWFLTLDDARSKLEDWRRYYNEQRPHGAIGNRPPITLHNRDSDPSSPS
VRGEWDVSIRRVCQILEVDRSTYHYKSRRPGQASLESKIKEICQTRVRYGYRRVHVLLRREGWLINQKKTRRIYRELGLQLRNKTPKRRVRAKLRDDRRD
ATHPNETWAMDFVHDQLATGNKIRVLTVVDLFSRYSPVLDPRFSYRAENVVQTLEHTWAAVGYPKTIRVDQGSEFISRELDLWAYSNGVTLDFSRPGKPT
DNAFIEAFNGRFRAECLNTHWFLTLDDARSKLEDWRRYYNEQRPHGAIGNRPPITLHNRDSDPSSPS
Blast result :
Comments
Genome in progress.
ISNisp3 is 96 % (ORFA) aa similar to ISBrsp1 and 81% (ORFB) aa similar to ISBrsp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISNisp3 is 96 % (ORFA) aa similar to ISBrsp1 and 81% (ORFB) aa similar to ISBrsp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Waterbury,J., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.