ISAtu5
- Family IS3
- Group IS407
Isoform Synonym(s) ISR1
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003063 | ND | Agrobacterium tumefaciens | Agrobacterium tumefaciens C58 |
DNA section
IS Length : 1271 bp
Ends
IR Length : 30/39
IRL : TGACCTGCCACTGCGAATTTCCTCCAGAATTGATTAGAGTCCGGCCCATC
IRR : TGACCTACCACTGAACTTTCATCCGGCAGCGATTAGAGCCTCTGCGGTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGATCTGAAC | CTTTCCGCCC | 0 |
DNA sequence
TGACCTGCCACTGCGAATTTCCTCCAGAATTGATTAGAGTCCGGCCCATCAGAGGACGGACGAATGAAGAAGCAGAGATTTACGGAAGAGCAGATCATCG
GTGTGCTGCGTGAGCAGGAGGCTGGCGCAAAGGCCGCCGACCTTTGCCGCAAGCACGGAATTTCAGAAGCGACGTTTTACAACTGGAAGGCCAAATACGG
CGGCATGGAAGTGTCCGAGGCGAAGCGCTTGAAGGCGCTCGAAGATGAGAATGCGAAGCTGAAGAAGTTGCTGGCCGAGCAGATGCTGGACGTTGCCGCG
CTCCGCGAGCTTCTTTCAAAAAAATGGTAGGGCCCGCCGCCAAGCGTGACGCGGTCACGCATCTGAAGGCCGTTATGGGGCTTTCGGAACGGCGGGCCTG
CCAGATTATATCCGCCGACCGCAAGATGATCCGCTACCGATCCTGCCGACCACCGGAAGTCGAGCTGCGATTGAGGCTGCGCGATCTGGCCAATGAGCGC
CGGCGTTTCGGATACCGACGGCTGTTCATTCTGCTTCGGCGCGATGGAGAGCCGTCCGGCGTCAATCGCATCTATCGACTGTATCGCGAGGAAGGTCTTT
CCGTTCGCAAGCGGAAAGCCAGGCGGCGCGCTGTCGGCACCCGTGCGCCGATTCTCGTCGAAGCAAAGGCAAATGCCCGCTGGTCGCTGGATTTCGTCCA
CGATCAGTTCGCCTGCGGCAGACGTTTCCGCGTGCTCAACATTGTCGATGATGTGACGCGCGAATGCCTGGCGGCGATCCCGGACACCTCGATCTCCGGC
CGCCGTGTGGCTCGTGAACTGACGGCACTGATACCACGGCGGGGGAAACCGGGAGTGATTGTTTCGGACAACGGCACAGAACTCACCTCGAACGCCATCC
TCGCCTGGTCGAAAGATCACAAGGTTGACTGGCACTACATCGCACCAGGAAGGCCAATGCAAAACGGCTATGTCGAGAGCTTCAACGGCCGCATGCGTGA
CGAGCTGCTGAATGAAAGCCTGTTCTTTGGTCTCGACCATGCCCGCAGCGCTATTGCTGAATGGGCGGACGACTACAACCATTTCCGACCGCACTCATCG
CTCGGATACCAGACCCCGGCAGCCTATGCCGGAACCATCGCCGCAACCGGCTCCAACGCCACGCAATATGGAAGCTTCGCGTTTCCGCCGGTTGCTCACA
CCGCGCCATTTGGCGTATCAAAAACCGCAGAGGCTCTAATCGCTGCCGGATGAAAGTTCAGTGGTAGGTCA
GTGTGCTGCGTGAGCAGGAGGCTGGCGCAAAGGCCGCCGACCTTTGCCGCAAGCACGGAATTTCAGAAGCGACGTTTTACAACTGGAAGGCCAAATACGG
CGGCATGGAAGTGTCCGAGGCGAAGCGCTTGAAGGCGCTCGAAGATGAGAATGCGAAGCTGAAGAAGTTGCTGGCCGAGCAGATGCTGGACGTTGCCGCG
CTCCGCGAGCTTCTTTCAAAAAAATGGTAGGGCCCGCCGCCAAGCGTGACGCGGTCACGCATCTGAAGGCCGTTATGGGGCTTTCGGAACGGCGGGCCTG
CCAGATTATATCCGCCGACCGCAAGATGATCCGCTACCGATCCTGCCGACCACCGGAAGTCGAGCTGCGATTGAGGCTGCGCGATCTGGCCAATGAGCGC
CGGCGTTTCGGATACCGACGGCTGTTCATTCTGCTTCGGCGCGATGGAGAGCCGTCCGGCGTCAATCGCATCTATCGACTGTATCGCGAGGAAGGTCTTT
CCGTTCGCAAGCGGAAAGCCAGGCGGCGCGCTGTCGGCACCCGTGCGCCGATTCTCGTCGAAGCAAAGGCAAATGCCCGCTGGTCGCTGGATTTCGTCCA
CGATCAGTTCGCCTGCGGCAGACGTTTCCGCGTGCTCAACATTGTCGATGATGTGACGCGCGAATGCCTGGCGGCGATCCCGGACACCTCGATCTCCGGC
CGCCGTGTGGCTCGTGAACTGACGGCACTGATACCACGGCGGGGGAAACCGGGAGTGATTGTTTCGGACAACGGCACAGAACTCACCTCGAACGCCATCC
TCGCCTGGTCGAAAGATCACAAGGTTGACTGGCACTACATCGCACCAGGAAGGCCAATGCAAAACGGCTATGTCGAGAGCTTCAACGGCCGCATGCGTGA
CGAGCTGCTGAATGAAAGCCTGTTCTTTGGTCTCGACCATGCCCGCAGCGCTATTGCTGAATGGGCGGACGACTACAACCATTTCCGACCGCACTCATCG
CTCGGATACCAGACCCCGGCAGCCTATGCCGGAACCATCGCCGCAACCGGCTCCAACGCCACGCAATATGGAAGCTTCGCGTTTCCGCCGGTTGCTCACA
CCGCGCCATTTGGCGTATCAAAAACCGCAGAGGCTCTAATCGCTGCCGGATGAAAGTTCAGTGGTAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 64 | 330 | + | No |
Description : First part of the transposase
ORF sequence :
MKKQRFTEEQIIGVLREQEAGAKAADLCRKHGISEATFYNWKAKYGGMEVSEAKRLKALEDENAKLKKLLAEQMLDVAALRELLSKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1005 bp | 334 aa | 249 | 1253 | + | No |
Description : Second part of the transposase
ORF sequence :
ECEAEEVAGRADAGRCRAPRASFKKMVGPAAKRDAVTHLKAVMGLSERRACQIISADRKMIRYRSCRPPEVELRLRLRDLANERRRFGYRRLFILLRRDG
EPSGVNRIYRLYREEGLSVRKRKARRRAVGTRAPILVEAKANARWSLDFVHDQFACGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELTALIPRRGK
PGVIVSDNGTELTSNAILAWSKDHKVDWHYIAPGRPMQNGYVESFNGRMRDELLNESLFFGLDHARSAIAEWADDYNHFRPHSSLGYQTPAAYAGTIAAT
GSNATQYGSFAFPPVAHTAPFGVSKTAEALIAAG
EPSGVNRIYRLYREEGLSVRKRKARRRAVGTRAPILVEAKANARWSLDFVHDQFACGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELTALIPRRGK
PGVIVSDNGTELTSNAILAWSKDHKVDWHYIAPGRPMQNGYVESFNGRMRDELLNESLFFGLDHARSAIAEWADDYNHFRPHSSLGYQTPAAYAGTIAAT
GSNATQYGSFAFPPVAHTAPFGVSKTAEALIAAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1190 bp | 396 aa | 64 | 1253 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKQRFTEEQIIGVLREQEAGAKAADLCRKHGISEATFYNWKAKYGGMEVSEAKRLKALEDENAKLKKLLAEQMLDVAALRELLSKKMVGPAAKRDAVTH
LKAVMGLSERRACQIISADRKMIRYRSCRPPEVELRLRLRDLANERRRFGYRRLFILLRRDGEPSGVNRIYRLYREEGLSVRKRKARRRAVGTRAPILVE
AKANARWSLDFVHDQFACGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELTALIPRRGKPGVIVSDNGTELTSNAILAWSKDHKVDWHYIAPGRPMQ
NGYVESFNGRMRDELLNESLFFGLDHARSAIAEWADDYNHFRPHSSLGYQTPAAYAGTIAATGSNATQYGSFAFPPVAHTAPFGVSKTAEALIAAG
LKAVMGLSERRACQIISADRKMIRYRSCRPPEVELRLRLRDLANERRRFGYRRLFILLRRDGEPSGVNRIYRLYREEGLSVRKRKARRRAVGTRAPILVE
AKANARWSLDFVHDQFACGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELTALIPRRGKPGVIVSDNGTELTSNAILAWSKDHKVDWHYIAPGRPMQ
NGYVESFNGRMRDELLNESLFFGLDHARSAIAEWADDYNHFRPHSSLGYQTPAAYAGTIAATGSNATQYGSFAFPPVAHTAPFGVSKTAEALIAAG
Blast result :
Comments
ISAtu5 is 93 % (ORFA) aa similar to ISOba1 and 85% (ORFB) aa similar to IS511. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Goodner,B., Hinkle,G., Gattung,S., Miller,N., Blanchard,M., Qurollo,B., Goldman,B.S., Cao,Y., Askenazi,M., Halling,C., Mullin,L., Houmiel,K., Gordon,J., Vaudin,M., Iartchouk,O., Epp,A., Liu,F., Wollam,C., Allinger,M., Doughty,D., Scott,C., Lappas,C., Markelz,B., Flanagan,C., Crowell,C., Gurson,J., Lomo,C., Sear,C., Strub,G., Cielo,C. and Slater,S. (2001) Science 294 (5550), 2323-2328.