ISAzvi12
- Family IS110
- Group IS1111
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP001157 | ND | Azotobacter vinelandii | Azotobacter vinelandii DJ |
DNA section
IS Length : 1248 bp
Ends
IR Length : 12
IRL : tgctgtgATGGACTCTCCCTGCACCACACTTATCCGCACCAACGGTGCCG
IRR : ----tatATGGACTCTCCCCACAAGTGGTGAGTAAAGCCTTTCCAACCCT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TCGGCTTGAA | TGAATTGTTA | 0 |
DNA sequence
TGCTGTGATGGACTCTCCCTGCACCACACTTATCCGCACCAACGGTGCCGTGAGTGATGTTCGGAGGATTGCAGCGATGCCCAAACAGATTGCAGTGGAT
TTGGCCAAGTCCGTTTACCAGGTTGCCGAGAGCGTCCATGCCGGCCACGTGAATCAGCGCAGACGGCTGGGTCGCGAGGCATTTCGCCGGTATATACAGG
AGCAGGCGGAACCGGTCGAGTGGCTGATGGAAGCCTGTGGCACGGCGCACTACTGGGGACGCTTCGCCCAGTCGCTGGGGCATCGGGTGATCCTGCTGCA
TCCGCGCTACGTGAAACCCTACCGGCGACGCAACAAGACCGACCGCAACGACTGCGACGCCCTCCTCGAAGCGGCGCGCTGCGCCGGCATTCATCCGGTG
CCGGTGAAGAGCCATGAGCAGCAGCAACTGCAACAGCTGCACGGCCTGCGCGAAGCTTGGAAGAAGAGCCGCACCCAGCGCATCAACCTGCTGCGCGGCA
TCTTCCGCGAGATGGGCCTTGAGGCGCCGGCGTCGATAGCTGCCTTCCTCCATGCAGCCAATGAACTGGTTGAGCAACCCGAAGTGGCGGCCCGGCGCGG
CCAACTGCAGATCGTCCTGGCGGAGATCAACCTGTACGAGCAATGCATGGTCGAGTGCGAGCAGCAGCTCGCCCGCTGGCATGCCGACGACGCCATCGTG
CGCAGGCTCGACGAAGTCAGCGGCATCGGTCTGCTGAGTGCCAGCGCCCTGAAAACGGCGGTCGGCCAGCCCGAGCGCTTTGCCAGTGGCCGCCAGTTGA
GCGCCTGGCTGGGCATGACGCCCCGCGAGTACAGCAGTGGCAATACCCGCAAGCTGGAACACATCAGCCGGCAGGGCAACGTCTATGTGCGCACCCTGCT
GATCCACGGCGCCGCCAGCAAGGCGGCCGTGGCGCTGGCCAACAAGCTGGTGCGGATCTGCTGGGCGATGTGGTGTCATGAGCGGCGCTTCAGCGGCGAC
TGGCAGAGCAGCAGGTTTTCCCGTGGTTGTGGTGAATAGCGACACTGTCGGAACAGGCGAGTCCTGCAGCGGAGCAAGGCCGATAACAATGCTGATCCTG
GTGATCGATTGAACGCTTGGCCCCCGCTGCGCGAACCACAGAGTGGCCCGGACGGTAATGTCCATGGAAGGCCGGATATATGAATGCAACCGCACTGGCG
ACAGGGTTGGAAAGGCTTTACTCACCACTTGTGGGGAGAGTCCATATA
TTGGCCAAGTCCGTTTACCAGGTTGCCGAGAGCGTCCATGCCGGCCACGTGAATCAGCGCAGACGGCTGGGTCGCGAGGCATTTCGCCGGTATATACAGG
AGCAGGCGGAACCGGTCGAGTGGCTGATGGAAGCCTGTGGCACGGCGCACTACTGGGGACGCTTCGCCCAGTCGCTGGGGCATCGGGTGATCCTGCTGCA
TCCGCGCTACGTGAAACCCTACCGGCGACGCAACAAGACCGACCGCAACGACTGCGACGCCCTCCTCGAAGCGGCGCGCTGCGCCGGCATTCATCCGGTG
CCGGTGAAGAGCCATGAGCAGCAGCAACTGCAACAGCTGCACGGCCTGCGCGAAGCTTGGAAGAAGAGCCGCACCCAGCGCATCAACCTGCTGCGCGGCA
TCTTCCGCGAGATGGGCCTTGAGGCGCCGGCGTCGATAGCTGCCTTCCTCCATGCAGCCAATGAACTGGTTGAGCAACCCGAAGTGGCGGCCCGGCGCGG
CCAACTGCAGATCGTCCTGGCGGAGATCAACCTGTACGAGCAATGCATGGTCGAGTGCGAGCAGCAGCTCGCCCGCTGGCATGCCGACGACGCCATCGTG
CGCAGGCTCGACGAAGTCAGCGGCATCGGTCTGCTGAGTGCCAGCGCCCTGAAAACGGCGGTCGGCCAGCCCGAGCGCTTTGCCAGTGGCCGCCAGTTGA
GCGCCTGGCTGGGCATGACGCCCCGCGAGTACAGCAGTGGCAATACCCGCAAGCTGGAACACATCAGCCGGCAGGGCAACGTCTATGTGCGCACCCTGCT
GATCCACGGCGCCGCCAGCAAGGCGGCCGTGGCGCTGGCCAACAAGCTGGTGCGGATCTGCTGGGCGATGTGGTGTCATGAGCGGCGCTTCAGCGGCGAC
TGGCAGAGCAGCAGGTTTTCCCGTGGTTGTGGTGAATAGCGACACTGTCGGAACAGGCGAGTCCTGCAGCGGAGCAAGGCCGATAACAATGCTGATCCTG
GTGATCGATTGAACGCTTGGCCCCCGCTGCGCGAACCACAGAGTGGCCCGGACGGTAATGTCCATGGAAGGCCGGATATATGAATGCAACCGCACTGGCG
ACAGGGTTGGAAAGGCTTTACTCACCACTTGTGGGGAGAGTCCATATA
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
963 bp | 320 aa | 77 | 1039 | + | No |
Chemistry : DEDD
ORF sequence :
MPKQIAVDLAKSVYQVAESVHAGHVNQRRRLGREAFRRYIQEQAEPVEWLMEACGTAHYWGRFAQSLGHRVILLHPRYVKPYRRRNKTDRNDCDALLEAA
RCAGIHPVPVKSHEQQQLQQLHGLREAWKKSRTQRINLLRGIFREMGLEAPASIAAFLHAANELVEQPEVAARRGQLQIVLAEINLYEQCMVECEQQLAR
WHADDAIVRRLDEVSGIGLLSASALKTAVGQPERFASGRQLSAWLGMTPREYSSGNTRKLEHISRQGNVYVRTLLIHGAASKAAVALANKLVRICWAMWC
HERRFSGDWQSSRFSRGCGE
RCAGIHPVPVKSHEQQQLQQLHGLREAWKKSRTQRINLLRGIFREMGLEAPASIAAFLHAANELVEQPEVAARRGQLQIVLAEINLYEQCMVECEQQLAR
WHADDAIVRRLDEVSGIGLLSASALKTAVGQPERFASGRQLSAWLGMTPREYSSGNTRKLEHISRQGNVYVRTLLIHGAASKAAVALANKLVRICWAMWC
HERRFSGDWQSSRFSRGCGE
Blast result :
Comments
The IR of ISAzvi12 are not at its termini. In the IS sequence, as given, 7nt separate IRl from the left-hand end of the element and 3nt separates IRr from the right-hand end. Like other IS1111 family, ISKpn4 subgroup members, ISAzvi12 targets a specific site in the 59be (or attC sites) in gene cassettes associated with integrons and is inserted in only one orientation.
ISAzvi12 is 79% aa similar to ISKpn4.
ISAzvi12 is 79% aa similar to ISKpn4.
References
1] Partridge and Hall (2003) J.Bacteriol. 185, 6371-6384
2] Post, V. and Hall, R.M. (2007) unpublished
2] Post, V. and Hall, R.M. (2007) unpublished