ISKpn7

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Klebsiella pneumoniae
Klebsiella pneumoniae
Pseudomonas aeruginosa plasmid
Enterobactericeae plasmid
DNA section
IS Length : 1956 bp

Ends


IR Length : 24/29

IRL : TGTTAGCAGCACTGTAAAACTGACCCACCCCAGCGAAGTAAAACTGATCC
IRR : TGTCAACACCAACTGAAAACTGACCCACTTTACCCCTGAATCACCAAAGT

Insertion site


Left flankDirect repeatRight flankDR Length
TTCAGCGGTTCCGGCGTACCCTC3

DNA sequence

TGTTAGCAGCACTGTAAAACTGACCCACCCCAGCGAAGTAAAACTGATCCACCTGGGCTAGCTTGGCGGCCTTTTCGAGGCCGTCGATGTTGACCCAGGA
GCAAACCGTGGAAATCAGAGTTCTTGCCCGACAGGGCCACTCCATCCGGCACATCGCCCGCACGTTGGGGGACTCGCGCAATACCGTGCGCCGCTACCTC
CGCAATCCGTCGGTAGCGCGATACCATCCCCGTGAACCACGCCCCACCAAACTGGGGGCATTTGAATCTTATCTGCGGCAACGGGTCGAGCAGGCCCACC
CCATCTGGCTGCCTGCCACCGTCCTGACTCGGGAAATCCGCGCTCAAGGGTATGGCGGCGGTCTCTCGTTATTGCGCGCCTTCCTCGCCACGCTCAAGCC
CGCCCGCCGCGAAGCAGGTCCGGCCGTCCGTTTGGAGACCGAGCCCGCACGGCAGTTGCAGGATGATTTCGTGGTCTTGCGCCGGGCCAGATCCCCCATG
TCGGCCTTCGTCGCCACGCTGGGATACAGCCGCATGACGTTTGTCACCTTCGTACCGGACGAATCCTTCGAGTCCGTGCGGGACTCCCTGTTGCTGGCTT
TTGACTATCTGGGCGGTGTGCCCCGCGAGGTGTTGTTCGACAACATGAAGACCGTGGTGCTGGAACGCGATGCCTACGGTGACGGCAAGCACCGTTTTCA
CCCCGGCTTGCTTCAACTGGCCGATGACCTGGGGTTCCGGATCCGGCTGTGTCGCCCGTATCGGGCCCAGACCAAAGGCAAGGTCGAGCGCTTCAATCGC
TACTTCCGCGAGAGCTTCTACAACCCGTTGCTGACCCGGATGAAGGGCACGGGGCTGCTGCTCGATTGTGCTGCTGCCAACCGACGCGTCCGTGACTGGT
TGGCAGACGAGGCGAATGTGCGGGTCCACGCCACCCTGAACGAGCGCCCCATCGACCGTTGGCGGCAGGAGCGGGAACACCTGCAACCCTTGCCATCCCG
TGTGCGGCGCGACGAGGCCCCCCTGCTGGATAACAGTCTCCGCCCGGTACCACTGGAATCTCTCCAGCGCGCACTGTCTGTCTATGACGCAATTGGGGAG
GCCTGCCGATGAACCTCCAGCATGAGCGGATCGACGCCCTGTGCCAGCAACTCAAACTCGAACGGGTGGCAACGCATTACCCCGTCCTGGCGCAGCAGGC
GGTGCGCGAGGACTTCAGCTTCACCGACTTCCTGGAACACCTGTTGCGGCATGAAGCGGGGTGTCGCCAGCAGCGCAGCCGCGAACTGCTGACGCGGATG
GCTTGCTTCCCGGGCATCAAGACCCTGGAGGACTATGACTTCAGCTTCAACCCCGGCGTGCCCAAGGCACTGGTGCAGGAGCTGGGCAGTCTGGCGTTTG
TCGAACGGGCCGAGAACGTGGTGCTGATCGGACCCTCTGGCATCGGCAAGACCCACCTCGCCATCGCCTTGGGTTACAAGGCCGCGCAAGCCGGGGTCAA
GACACGCTTCATCACGGCAGCCGACCTGATGCTGCAACTGAGCACGGCGCAGCGACAAGGGCGGCTGAAGGAATACTTCAACCGCGCCATCCTCGGTCCA
CGGCTGCTGGTGATCGATGAGATCGGCTACCTGCCTTTCGGACGCGAGGAAGCGAACCTGTTCTTTCAGGTGGTGGCGAAGCGCTATGAGAAAGGGGCGA
TGATCCTGACCTCGAACCTGCCGTTCAGCCAGTGGGCCGACGCGTTTGCCGGCGATACCACCCTGACAGCCGCGATGCTGGATCGGCTGCTGCACCATGC
CCATATCCTGACCCTGAGCGGCGAAAGCTACCGCTTGAAGGACAAGAGGAAGGCGGGAGTGGTCAGGAAAAATTCCAAACCCGAATGATCCAGGTGGGTC
AGTATTACTTTGGTGATTCAGGGGTAAAGTGGGTCAGTTTTCAGTTGGTGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1026 bp341 aa871112+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLTQEQTVEIRVLARQGHSIRHIARTLGDSRNTVRRYLRNPSVARYHPREPRPTKLGAFESYLRQRVEQAHPIWLPATVLTREIRAQGYGGGLSLLRAFL
ATLKPARREAGPAVRLETEPARQLQDDFVVLRRARSPMSAFVATLGYSRMTFVTFVPDESFESVRDSLLLAFDYLGGVPREVLFDNMKTVVLERDAYGDG
KHRFHPGLLQLADDLGFRIRLCRPYRAQTKGKVERFNRYFRESFYNPLLTRMKGTGLLLDCAAANRRVRDWLADEANVRVHATLNERPIDRWRQEREHLQ
PLPSRVRRDEAPLLDNSLRPVPLESLQRALSVYDAIGEACR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
780 bp259 aa11091888+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHLLRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVER
AENVVLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILGPRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMIL
TSNLPFSQWADAFAGDTTLTAAMLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE

 

Blast result :
Comments
ISKpn7 is 68% (ORFA) and 85% (ORFB) aa similar to ISRso19 .
References
1] Thierry Naas (2007) Direct submission.