ISVpa3

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s) ISV3L, ISV5L, ISV5R
Accession numberTranspositionOriginHost
ND Vibrio parahaemolyticus
Vibrio parahaemolyticus
Vibrio parahaemoliticus AQ3860
Vibrio parahaemoliticus AQ3776
DNA section
IS Length : 1054 bp

Ends


IR Length : 19

IRL : GGCTTTGTTGCGTAATTCGAGGGAACTAAATCAGAAGCTTACGATCTGAT
IRR : GGCTTTGTTGCGTAATTCGGTCAGAAAACCAAACTGCCTGCAATTGAGCA

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GGCTTTGTTGCGTAATTCGAGGGAACTAAATCAGAAGCTTACGATCTGATCGGTTAAGTAAGTTAATTCCCCTAGTCTCATGCCGAAACCTCGCTACAAA
ACAACGAACTGGAAACAGTACAACAAAGCCTTAATCAACCGTGGTTCACTCACTTTCTGGATTGATGAAGAAGCCATTCGCCAGTGGAAGCAGAGTAAAC
AAGATAAACGTGGTAGACCTCGTCAGTTCAGCGACTTAGCCATTACCACTGCGCTTATGGTGAAACGAGTTTTCTCAATGCCGTTGAGAGCATTGCAAGG
GTTTATCGATTCTGTTTTTTCATTGGCTAACGTCCCTATCGTCTGTCCCCATTACAGCTGTATCAGTCGAAGAGCTAAGCAAGTCGAGGTTTCATTTAAA
CCCAAGACTAGAGGAGCAATACAGCATCTAGCTATTGATGCGACGGGTCTCAAGGTTTATGGCGAAGGTGAATGGAAGGTCAAGAAGCATGGTACTGACG
GGAAGCGTAGGGTCTGGAGAAAATTGCATTTAGCGGTAGACACCAGCACTCATGAAATCGTCGCGGCAGAACTGAGTTTATCTAATGTTACCGATGCAGA
AGTCCTTCCTAACTTGCTCAAGCAAACACGCCGAAGAATTATCGAGATATCTGGTGATGGCGCTTATGACACAAGGGATTGCCACGATGCCATACGGTTC
AAGCGAGCTGTTCCACTTATCCCTCCAAGAGAAGGGGCAGCCTTCTGGGAGAATGGTCACCCTAGGAATTTAGCGGTAGGTTGCCAGAAGCTCTACGGCT
CCAACAATAAGTGGAAAAAGCGGTACGGCTATCATAAACGCTCACTATCAGAGACAGCAATGTATCGAGTGAAGCAGTTGTTAGGTGGGCGATTAAGTCT
GAGAAACTACAATGCTCAGGTTGGTGAAACTTACGCCATGATTAAAGCGCTGAACAAACTTACAGGGCTTGGTATGCCTGAAACTCAGTGTATTGTATAA
GAATTGCTCAATTGCAGGCAGTTTGGTTTTCTGACCGAATTACGCAACAAAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
921 bp306 aa801000+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKPRYKTTNWKQYNKALINRGSLTFWIDEEAIRQWKQSKQDKRGRPRQFSDLAITTALMVKRVFSMPLRALQGFIDSVFSLANVPIVCPHYSCISRRAK
QVEVSFKPKTRGAIQHLAIDATGLKVYGEGEWKVKKHGTDGKRRVWRKLHLAVDTSTHEIVAAELSLSNVTDAEVLPNLLKQTRRRIIEISGDGAYDTRD
CHDAIRFKRAVPLIPPREGAAFWENGHPRNLAVGCQKLYGSNNKWKKRYGYHKRSLSETAMYRVKQLLGGRLSLRNYNAQVGETYAMIKALNKLTGLGMP
ETQCIV

 

Blast result :
Comments
ISVpa3 is 96% aa similar to ISVa2.
ISVpa3 has transposition activity as demonstrated by a replicon fusion assay
References
1] Mitsuaki Nishibuchi. (2007) Direct submission.
2] Terai, A., Baba, K., Shirai, H., Yoshida, O., Takeda, Y., and Nishibuchi, M. (1991) J. Bacteriol. 173, 5036-5046.