ISKpn8
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| EF382672 | ND | Klebsiella pneumoniae | Enterobacter cloacae plasmid pECIMPQ Klebsiella pneumoniae NK29 plasmid pK29 Enterobacter cloacae plasmid pECIMP |
DNA section
IS Length : 1443 bp
Ends
IR Length : 22/31
IRL : TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGTCCCTGATATG
IRR : TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCACATAGGTCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTTTTCAATTA | ACC | CGAGGCCCCTGTATG | 3 |
| ATTTTTCAATTA | ACC | CGAGGCCCCTGTATG | 3 |
DNA sequence
TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGTCCCTGATATGTCAAAGCCAAAATACCCCTTCGAAAAGCGCCTTGAAGTCGTGAATCACTA
CTTCACAACTGATGATGGTTACAGGATCATCTCTGCACGTTTTGGTGTGCCTCGAACCCAGGTCAGGACGTGGGTTGCCCTCTATGAAAAACATGGAGAA
AAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGCATTAAGGTCGTGAAAGCCGTGACCGAGCAGCAGATGTCCCTCAATCAGG
CTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAAGTGGCTGAAAGTCTATGAGGAGCACGGAGAAGCTGGTTTACGCGCACTCAAGATCGG
CACCAAAAGAAACATTACAATGTCAGTTGATCCAGAAAAAGCGGCGTTAGCATTGGAGCTGTCGAAAGACCGACGTATTGAGGATCTTGAAAGACAAGTT
CGATTCCTTGAAATGCGGCTTACGTATCTAAAAAAGCTGAAAGCCTTAGCTCATCCCGCGAAAAAGTGAAAGTACTCAACGAGCTAAGGCAGTTTTACCC
TCTTGATGAGCTTCTCAAGGCAGCGGAGATACCGCGCAGTACGTTTTATTACCATCTAAAGGCTCTCAGCAAGCCTGATAAGTATGCGGATGTTAAAAAG
CGTATTGGTGAGATTTATCACGAGAATAAAGGTCGTTACGGATACCGTAGGGTAACGTTGTCTCTCCATCGAGACGGGGAACGGATTAACCATAAAGCCG
TTCAGCGCCTGATGGGAACCCTCTCGCTTAAGGCAGCGATTAAGGTCAAGCGATACAGCTCCTACAGAGGAGAGGTAGGGCAAACCGCCCCCAATGTTCT
CCAAAGGGATTTCAAGGCTACACGGCCAAACGAGAAGTGGGTTACCGACGTTACTGAATTTGCAGTCAATGGGCGAAAGCTGTATTTGTCTCCAGTAATA
GATCTCTTCAACAACGAAGTTATTTCTTACAGCCTGTCGGAAAGACCTGTGATGAACATGGTCGAGAATATGCTCGATCAGGCATTCAAAAAGCTTAAGC
CTCACGAGCATCCTATTCTGCACTCTGACCAGGGATGGCAGTATCGTATGAGAAGGTATCAAAACATCCTTAAAGAACAAGGCATTACACAGAGCATGTC
CAGAAAAGGCAATTGTCTGGATAATGCAGTCGTGGAATGTTTCTTTGGAACCTTAAAGTCGGAGTGTTTTTATCTTGACGAGTTCAGTAATATAAGCGAA
CTGAAGGATGCTGTTACGGAATATATTGACTACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGAGTCCAATTGAATATCGAACCCAGACCT
ATGTGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA
CTTCACAACTGATGATGGTTACAGGATCATCTCTGCACGTTTTGGTGTGCCTCGAACCCAGGTCAGGACGTGGGTTGCCCTCTATGAAAAACATGGAGAA
AAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGCATTAAGGTCGTGAAAGCCGTGACCGAGCAGCAGATGTCCCTCAATCAGG
CTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAAGTGGCTGAAAGTCTATGAGGAGCACGGAGAAGCTGGTTTACGCGCACTCAAGATCGG
CACCAAAAGAAACATTACAATGTCAGTTGATCCAGAAAAAGCGGCGTTAGCATTGGAGCTGTCGAAAGACCGACGTATTGAGGATCTTGAAAGACAAGTT
CGATTCCTTGAAATGCGGCTTACGTATCTAAAAAAGCTGAAAGCCTTAGCTCATCCCGCGAAAAAGTGAAAGTACTCAACGAGCTAAGGCAGTTTTACCC
TCTTGATGAGCTTCTCAAGGCAGCGGAGATACCGCGCAGTACGTTTTATTACCATCTAAAGGCTCTCAGCAAGCCTGATAAGTATGCGGATGTTAAAAAG
CGTATTGGTGAGATTTATCACGAGAATAAAGGTCGTTACGGATACCGTAGGGTAACGTTGTCTCTCCATCGAGACGGGGAACGGATTAACCATAAAGCCG
TTCAGCGCCTGATGGGAACCCTCTCGCTTAAGGCAGCGATTAAGGTCAAGCGATACAGCTCCTACAGAGGAGAGGTAGGGCAAACCGCCCCCAATGTTCT
CCAAAGGGATTTCAAGGCTACACGGCCAAACGAGAAGTGGGTTACCGACGTTACTGAATTTGCAGTCAATGGGCGAAAGCTGTATTTGTCTCCAGTAATA
GATCTCTTCAACAACGAAGTTATTTCTTACAGCCTGTCGGAAAGACCTGTGATGAACATGGTCGAGAATATGCTCGATCAGGCATTCAAAAAGCTTAAGC
CTCACGAGCATCCTATTCTGCACTCTGACCAGGGATGGCAGTATCGTATGAGAAGGTATCAAAACATCCTTAAAGAACAAGGCATTACACAGAGCATGTC
CAGAAAAGGCAATTGTCTGGATAATGCAGTCGTGGAATGTTTCTTTGGAACCTTAAAGTCGGAGTGTTTTTATCTTGACGAGTTCAGTAATATAAGCGAA
CTGAAGGATGCTGTTACGGAATATATTGACTACTACAACAGCAGAAGAATTAGCCTGAAATTAAAAGGTCTGAGTCCAATTGAATATCGAACCCAGACCT
ATGTGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 522 bp | 173 aa | 48 | 569 | + | No |
Description : First part of the transposase
ORF sequence :
MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVALYEKHGEKGLIPKPKGVSADPELRIKVVKAVTEQQMSLNQAAAHFMLAGSGSVAKW
LKVYEEHGEAGLRALKIGTKRNITMSVDPEKAALALELSKDRRIEDLERQVRFLEMRLTYLKKLKALAHPAKK
LKVYEEHGEAGLRALKIGTKRNITMSVDPEKAALALELSKDRRIEDLERQVRFLEMRLTYLKKLKALAHPAKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 906 bp | 301 aa | 512 | 1417 | + | No |
Description : Second part of the transposase
ORF sequence :
NAAYVSKKAESLSSSREKVKVLNELRQFYPLDELLKAAEIPRSTFYYHLKALSKPDKYADVKKRIGEIYHENKGRYGYRRVTLSLHRDGERINHKAVQRL
MGTLSLKAAIKVKRYSSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLKPHEH
PILHSDQGWQYRMRRYQNILKEQGITQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIDYYNSRRISLKLKGLSPIEYRTQTYVPR
V
MGTLSLKAAIKVKRYSSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLKPHEH
PILHSDQGWQYRMRRYQNILKEQGITQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIDYYNSRRISLKLKGLSPIEYRTQTYVPR
V
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1370 bp | 456 aa | 48 | 1417 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVALYEKHGEKGLIPKPKGVSADPELRIKVVKAVTEQQMSLNQAAAHFMLAGSGSVAKW
LKVYEEHGEAGLRALKIGTKRNITMSVDPEKAALALELSKDRRIEDLERQVRFLEMRLTYLKKAESLSSSREKVKVLNELRQFYPLDELLKAAEIPRSTF
YYHLKALSKPDKYADVKKRIGEIYHENKGRYGYRRVTLSLHRDGERINHKAVQRLMGTLSLKAAIKVKRYSSYRGEVGQTAPNVLQRDFKATRPNEKWVT
DVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLKPHEHPILHSDQGWQYRMRRYQNILKEQGITQSMSRKGNCLDNAVVECFF
GTLKSECFYLDEFSNISELKDAVTEYIDYYNSRRISLKLKGLSPIEYRTQTYVPRV
LKVYEEHGEAGLRALKIGTKRNITMSVDPEKAALALELSKDRRIEDLERQVRFLEMRLTYLKKAESLSSSREKVKVLNELRQFYPLDELLKAAEIPRSTF
YYHLKALSKPDKYADVKKRIGEIYHENKGRYGYRRVTLSLHRDGERINHKAVQRLMGTLSLKAAIKVKRYSSYRGEVGQTAPNVLQRDFKATRPNEKWVT
DVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLKPHEHPILHSDQGWQYRMRRYQNILKEQGITQSMSRKGNCLDNAVVECFF
GTLKSECFYLDEFSNISELKDAVTEYIDYYNSRRISLKLKGLSPIEYRTQTYVPRV
Blast result :
Comments
ISKpn8 is 96% aa similar to IS150.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chen,Y.T., Lauderdale,T.L., Liao,T.L., Shiau,Y.R., Shu,H.Y., Wu,K.M., Yan,J.J., Su,I.J. and Tsai,S.F. (2007) Antimicrob. Agents Chemother. 51 (8), 3004-3007.