ISRhosp8

  • Family IS110
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008271 ND Rhodococcus sp.
Rhodococcus sp. RHA1 plasmid pRHL3
DNA section
IS Length : 1439 bp

Ends


Left end : CCCGTTACGTTATTTACGGTCCTTCACAGAAGGGCTACGGTCGCCAGACCCGGTATGACTCCTTGCCCCTGTCTCACGCATGATCCGGGGGGTGGTGTCG
Right end : ACGCCGCTGCGACGTCCTCTACGCCATGCTGAAGAACAAGGAACTCTACCGGGCACCACTCCCCTCGGCGGCTTGACGGGAACCATAGGGACACCCCCCG

Insertion site


Left flankDirect repeatRight flankDR Length
TTCAGGTGCCGAAACCGCGGCGTTGCCGCCGTAGCTCCTCG0

DNA sequence

CCCGTTACGTTATTTACGGTCCTTCACAGAAGGGCTACGGTCGCCAGACCCGGTATGACTCCTTGCCCCTGTCTCACGCATGATCCGGGGGGTGGTGTCG
CCGGGTGTGGTGGCGTCGACGGACCGCTGATAGGGACACGGCCAACCGAAGTTTGGGTAGGTCCCTTTGTAACGTGGGTGCCGGGCGTCGATGCCGGATC
GGCGACCGACCGACCGGAGACACAAGGGGCGAACCCTGAATGGACTCGAACTATGCGGTGTACTGCGGCATCGACGTCGGCAAAGGTGAGCACCACGCCG
TCGGACTGACCGCCGCCGGAAAGAAAATCTACGACAAGGCCCTGCCGAACGACGAAGCGAAGCTGCGGGCCAGCCACGGATCGGTGCTGGTGGTCGTCGA
CCAACCCAATACGATCGGCGCCCTGCCGGTCACCGTGGCCCGCGCCTGCAGCCACGAGGTCGGCTACCTGCCCGGACTGGCGATGCGCCGCATCGCCGAC
CTATACCCCGGACAAGCGAAAACAGACGCCCGAGACGCCTTCATCATTGCCGATACCGCCCGCACCATGCCGCACACCCTGCGCCGGGTCGACACCGGCG
ACGAGGCCCTCGCCGAGCTCGAGGTCCTCATCGGGTTCGACGACGACCTCGCCGGCGAGGCCACCCGGGTCAGCAACCGCATCCGCGGACTCCTCACCGG
AATCCATCCAGCCCTCGAACGGGTCCTCGGCCCCCGCATCGCCCATCCGGCGGTCCTCGAGATCCTCTCCCGCTGCGGGGGACCGGCCGGAATCCGCAAG
GCCGGCAAGCGCAAGCTCACCGCGATCGCGGTCAAACACGCGCCCCGCATGGGCACCCGACTCGTCGGCGAGATCCTTGCCGCCCTCGAAGAGCAGACCG
TAATTGTCCCCGGCAGCACGGCCGCCGAGACCATCCTCCCCAAACTCGCCGACACCCTGAAAGAGACTCTCCAGCAACGGAAATCGGTCGCTGAGGACGT
GGAGGGGGCCCTCGATGACCACCCTCTTTCCCGAGTCCTGACCTCGATGCCCGGCATCGGTTTCAGGACTGCAGCCCGGATCCTGATCGAGGTCGGCGAC
GGCTCCGCCTTCGCCTCCGCCGCCCACCTTGCCGCCTACGCAGGCATCGCACCGGTCACCCGCAAATCGGGCTCCTCGATCCGAGGCGAACACCCGGCCC
GATCCGGCAACAAAAAGCTCAAACGAGCGCTGTTCCTCTCCGCGTTCTCCGCCCTCCACGACCCCACCAGTCGCGCCTACTACGACAAAAAGCGTGCCGA
GAAGAAAAAACACAACGCGGCCCTGATCTGCCTCGCCCGACGCCGCTGCGACGTCCTCTACGCCATGCTGAAGAACAAGGAACTCTACCGGGCACCACTC
CCCTCGGCGGCTTGACGGGAACCATAGGGACACCCCCCG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1176 bp391 aa2401415+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MDSNYAVYCGIDVGKGEHHAVGLTAAGKKIYDKALPNDEAKLRASHGSVLVVVDQPNTIGALPVTVARACSHEVGYLPGLAMRRIADLYPGQAKTDARDA
FIIADTARTMPHTLRRVDTGDEALAELEVLIGFDDDLAGEATRVSNRIRGLLTGIHPALERVLGPRIAHPAVLEILSRCGGPAGIRKAGKRKLTAIAVKH
APRMGTRLVGEILAALEEQTVIVPGSTAAETILPKLADTLKETLQQRKSVAEDVEGALDDHPLSRVLTSMPGIGFRTAARILIEVGDGSAFASAAHLAAY
AGIAPVTRKSGSSIRGEHPARSGNKKLKRALFLSAFSALHDPTSRAYYDKKRAEKKKHNAALICLARRRCDVLYAMLKNKELYRAPLPSAA

 

Blast result :
Comments
ISRhosp8 is 75% aa similar to ISSfl4.
References
1] McLeod,M.P., Warren,R.L., Hsiao,W.W., Araki,N., Myhre,M., Fernandes,C., Miyazawa,D., Wong,W., Lillquist,A.L., Wang,D., Dosanjh,M., Hara,H., Petrescu,A., Morin,R.D., Yang,G., Stott,J.M., Schein,J.E., Shin,H., Smailus,D., Siddiqui,A.S., Marra,M.A., Jones,S.J., Holt,R., Brinkman,F.S., Miyauchi,K., Fukuda,M., Davies,J.E., Mohn,W.W. and Eltis,L.D. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15582-15587.