ISKpn10
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ971342 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae plasmid pRDDHA |
DNA section
IS Length : 1198 bp
Ends
IR Length : 12
IRL : GGTAATCCCCCCGAAAAACCAGGGTGCTCATAAGTAGAATTTTTCCGTAC
IRR : GGTAATCCCCCCATTTTTAGGGGCATTCAAAAGTAGAGGTCATGCGGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACCCAAGTG | GGGC | AGCCCAAGTG | 4 |
| TGTGCCACTG | AAAT | CACCCGATTA | 4 |
| GCGCACACCG | ATCT | GTCGCAAGGC | 4 |
| ACCGTCAGCA | CCCC | CATCAAGATG | 4 |
| TGGCTGTTCT | AACCCATGAC | 0 |
DNA sequence
GGTAATCCCCCCGAAAAACCAGGGTGCTCATAAGTAGAATTTTTCCGTACCTTGAACGCAGGAGGTTCTACATGAAAACATCACGCTTTAGCGATACCCA
GATCATCGCCATCCTCAAACAAGCTGAGGCTGGAGCTCCCGTCCCGGAGCTCTGCCGCGAGCATGGCATCAGCACGACCACCTTCTACAAGTGGCGTTCC
AAATTCGGGGGGATGGATGCCTCCCTCATGGCTCGACTCAAGGAGCTGGAGGACGAAAACCGCCGCCTCAAGAAGATGTACGCCGAGGAGCGCCTCAAGG
CAGAGATCATCACCGAGGCCATGGCAAAAAAGTGGTAACGCCATCTGCCCGCCGGGAGATGGCGACCAAAGCCGTGACTCACTACGCCATATCCATCCGT
CTGGCTTGCCGGATCTTCAAGGTCAGTGAAAGCTGCTATCGCTATCAGCCCGTGCTGGTCGATGAAAACCAGGAAATTGCCGATTGGCTGCTACGTATCA
CGGCCAGCCAGCGCAACTGGGGCTTCGGGTTATGCTTTTTGTACCTGCGCAACGTCCAAGGCTTTGGCTGGAATCACAAACGGGTATACCGGATCTATCG
GGCGCTGGAGCTCAATCTGCGGATAAAACCCAGGAAACGGTTGGTTCGGGCTAAGCCAGAGCCGTTGGCGGTTCCCGACCAGCCTAATCAGTGCTGGTCG
ATGGACTTTATGCATGACCAGCTCAGCGATGGCCGCTCGGTGCGGCTGTTTAATGTGATCGATGACTTCAATCGTGAAGCGCTGTGCATCGAGGTGGATT
TCTCCTTACCCGCCTCACGAGTCAAACGGGCGCTGGAGCAGGTGATCACGTGGCGAGGTAAGCCCGCCATTATCCGATGTGACAATGGCCCGGAATACAT
CAGCGAAGAGCTCAAGCGATGGGCGAAACAGGAGGGGATAACCCTGGGCTATATCCAGCCAGGAAATCCGCAGCAGAATGCCTACATCGAACGGTTTAAC
CGCACGGTGCGCTACGACTGGCTGGGCCATTACCTGTTTGAGTCACTGAATGAACTACAGGAGTTTGCCACGAACTGGCTGTGGGTTTATAACCATGAGC
GGCCCAATATGGCGCTGGGTGGCTACACGCCGAAACAGCGTCTGGCACAGGCCGCATGACCTCTACTTTTGAATGCCCCTAAAAATGGGGGGATTACC
GATCATCGCCATCCTCAAACAAGCTGAGGCTGGAGCTCCCGTCCCGGAGCTCTGCCGCGAGCATGGCATCAGCACGACCACCTTCTACAAGTGGCGTTCC
AAATTCGGGGGGATGGATGCCTCCCTCATGGCTCGACTCAAGGAGCTGGAGGACGAAAACCGCCGCCTCAAGAAGATGTACGCCGAGGAGCGCCTCAAGG
CAGAGATCATCACCGAGGCCATGGCAAAAAAGTGGTAACGCCATCTGCCCGCCGGGAGATGGCGACCAAAGCCGTGACTCACTACGCCATATCCATCCGT
CTGGCTTGCCGGATCTTCAAGGTCAGTGAAAGCTGCTATCGCTATCAGCCCGTGCTGGTCGATGAAAACCAGGAAATTGCCGATTGGCTGCTACGTATCA
CGGCCAGCCAGCGCAACTGGGGCTTCGGGTTATGCTTTTTGTACCTGCGCAACGTCCAAGGCTTTGGCTGGAATCACAAACGGGTATACCGGATCTATCG
GGCGCTGGAGCTCAATCTGCGGATAAAACCCAGGAAACGGTTGGTTCGGGCTAAGCCAGAGCCGTTGGCGGTTCCCGACCAGCCTAATCAGTGCTGGTCG
ATGGACTTTATGCATGACCAGCTCAGCGATGGCCGCTCGGTGCGGCTGTTTAATGTGATCGATGACTTCAATCGTGAAGCGCTGTGCATCGAGGTGGATT
TCTCCTTACCCGCCTCACGAGTCAAACGGGCGCTGGAGCAGGTGATCACGTGGCGAGGTAAGCCCGCCATTATCCGATGTGACAATGGCCCGGAATACAT
CAGCGAAGAGCTCAAGCGATGGGCGAAACAGGAGGGGATAACCCTGGGCTATATCCAGCCAGGAAATCCGCAGCAGAATGCCTACATCGAACGGTTTAAC
CGCACGGTGCGCTACGACTGGCTGGGCCATTACCTGTTTGAGTCACTGAATGAACTACAGGAGTTTGCCACGAACTGGCTGTGGGTTTATAACCATGAGC
GGCCCAATATGGCGCTGGGTGGCTACACGCCGAAACAGCGTCTGGCACAGGCCGCATGACCTCTACTTTTGAATGCCCCTAAAAATGGGGGGATTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 264 bp | 88 aa | 72 | 335 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSRFSDTQIIAILKQAEAGAPVPELCREHGISTTTFYKWRSKFGGMDASLMARLKELEDENRRLKKMYAEERLKAEIITEAMAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1032 bp | 343 aa | 128 | 1159 | + | No |
Description : Second part of the transposase
ORF sequence :
GWSSRPGALPRAWHQHDHLLQVAFQIRGDGCLPHGSTQGAGGRKPPPQEDVRRGAPQGRDHHRGHGKKVVTPSARREMATKAVTHYAISIRLACRIFKVS
ESCYRYQPVLVDENQEIADWLLRITASQRNWGFGLCFLYLRNVQGFGWNHKRVYRIYRALELNLRIKPRKRLVRAKPEPLAVPDQPNQCWSMDFMHDQLS
DGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRALEQVITWRGKPAIIRCDNGPEYISEELKRWAKQEGITLGYIQPGNPQQNAYIERFNRTVRYDWLG
HYLFESLNELQEFATNWLWVYNHERPNMALGGYTPKQRLAQAA
ESCYRYQPVLVDENQEIADWLLRITASQRNWGFGLCFLYLRNVQGFGWNHKRVYRIYRALELNLRIKPRKRLVRAKPEPLAVPDQPNQCWSMDFMHDQLS
DGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRALEQVITWRGKPAIIRCDNGPEYISEELKRWAKQEGITLGYIQPGNPQQNAYIERFNRTVRYDWLG
HYLFESLNELQEFATNWLWVYNHERPNMALGGYTPKQRLAQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1088 bp | 362 aa | 72 | 1159 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSRFSDTQIIAILKQAEAGAPVPELCREHGISTTTFYKWRSKFGGMDASLMARLKELEDENRRLKKMYAEERLKAEIITEAMAKKVVTPSARREMATK
AVTHYAISIRLACRIFKVSESCYRYQPVLVDENQEIADWLLRITASQRNWGFGLCFLYLRNVQGFGWNHKRVYRIYRALELNLRIKPRKRLVRAKPEPLA
VPDQPNQCWSMDFMHDQLSDGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRALEQVITWRGKPAIIRCDNGPEYISEELKRWAKQEGITLGYIQPGNP
QQNAYIERFNRTVRYDWLGHYLFESLNELQEFATNWLWVYNHERPNMALGGYTPKQRLAQAA
AVTHYAISIRLACRIFKVSESCYRYQPVLVDENQEIADWLLRITASQRNWGFGLCFLYLRNVQGFGWNHKRVYRIYRALELNLRIKPRKRLVRAKPEPLA
VPDQPNQCWSMDFMHDQLSDGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRALEQVITWRGKPAIIRCDNGPEYISEELKRWAKQEGITLGYIQPGNP
QQNAYIERFNRTVRYDWLGHYLFESLNELQEFATNWLWVYNHERPNMALGGYTPKQRLAQAA
Blast result :
Comments
ISKpn10 is 94% (ORFA) and 80% (ORFB) aa similar to ISXcd1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Verdet,C., Benzerara,Y., Gautier,V., Adam,O., Ould-Hocine,Z. and Arlet,G. (2006) Antimicrob. Agents Chemother. 50 (2), 607-617