ISfinder

ISKpn14

Family IS1
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP000649	ND	Klebsiella pneumoniae	Klebsiella pneumoniae subsp pneumoniae MGH78578 plasmid pKPN4

DNA section

IS Length : 768 bp

Ends

IR Length : 18/23

IRL : GGTGATGCTACCAACTTGCTGATTTAGTGTATAATGGTGTTTTTGAGGTG
IRR : GGTAATGACTCCAATTTACTGATAGTGTTTTATGTTCAGATAGTGCCCGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGTATCAGTTTGGCGTGCG		GGCTTCGAGAAGCGCGAG	0

DNA sequence

GGTGATGCTACCAACTTGCTGATTTAGTGTATAATGGTGTTTTTGAGGTGCTCCCGTGGCTTCAGTCTCCGTCTGCTGTCCCTCCTGTTCCGCTACTGAA
GGCGTGATGCGTAATGGCAAAAGTACTGCCGGGCATCAACGTTATCTCTGCTCTCACTGCCGTAAAACATGGCAGCTCACCTTCACTTATGCCGCTTCTC
AGCCCGGTACACACCAGAAAATCATTGATATGGCTATGAACGGCGTCGGTTGCCGTGCCACCGCACGACTAATGGGCGTGGGCCTCAACACCATTTTGCG
CCATTTAAAAAACTCAGGCCGCAGTCAGTAACCTCCCGGATACAGCCGGGCAGTGATGTCATTGTCTGTGCGGAGATGGACGAGCAATGGGGCTACGTCG
GTGCAAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGCAGGGCGGTGGTAGCCCACGTTTTCGGTGAACGCACTATGGCCACACTGGA
GCGTCTTCTGAGCCTGCTGTCAGTTTTTGACGTGGTGGTATGGATGACGGACGGCTGGCCGATGTATGAGTCACGTCTGAAGGGAAAACTGCACGTCATC
AGTAAGCGTTACACGCAGCGAATTGAGCGACACAACCTGAATCTGAGACAGCATCTGGCAAGGCTGGGCAGGAAGTCCCTGTCGTTCTCAAAATCGGTGG
AGCTGCATGACAAAGTCATCGGGCACTATCTGAACATAAAACACTATCAGTAAATTGGAGTCATTACC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
276 bp	91 aa	56	331	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

VASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTYAASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHLKNSGRSQ

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
525 bp	174 aa	229	753	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

YGYERRRLPCHRTTNGRGPQHHFAPFKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVF
DVVVWMTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
698 bp	274 aa	56	753	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTYAASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHLKNSGRSQPGTHQKIID
MAMNGVGCRATARLMGVGLNTILRHLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVF
DVVVWMTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Blast result :

Comments

ISKPn14 is 89% identical to IS1X2. Orf1= InsA: 91 aa; Orf2 = InsB ; Orf3= InsAB':274 aa,received by a -1 translational frameshift

References

1] Escoubas, J.M., Prère, M.F., Fayet, O., Salvignol, I., Galas, D., Zerbib, D., and Chandler, M. (1991) EMBO J. 10, 705-712.
2] Umeda, M., and Ohtsubo, E. (1991) Gene 98, 1-5.
3] Sekine, Y., and Ohtsubo, E. (1989) Proc. Natl. Acad. Sci., USA 86, 4609-4613.