ISKpn12
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| EU780013 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae 12836 |
DNA section
IS Length : 842 bp
Ends
IR Length : 16/18
IRL : GGGTCAGGACCCATTGATTTGAACTGACGGCTGTGATTCAGATGGGCGTA
IRR : GGGTCAGGACTCATTGCTCATAGCCAGAAGATAACGGTCGCAGCGAGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGGTCAGGACCCATTGATTTGAACTGACGGCTGTGATTCAGATGGGCGTATAGGAGCCCGACTGATGAGTAATTTGTTTTGGCTGACGGACGAGCAAATG
GCTCGTCTTCAGCCCTATTTCCCCAAGAGCCATGGCCGCCAGCGCGTTGATGATCGACGCGTTCTGAGCGGCATCATCTTCGTCAACCGCAACGGGCTGC
GTTGGCGTGATGCGCCGAAGGAATATGGCCCGGCGAAGACGCTTTATAACCGCTGGAAACGCTGGGGCGACAAGGGCGTATTTATCCAGATGATGGAAGG
TCTGGCCGTGCCTGAAGCCGCAGAGCACAAGACCATCATGATCGATGCGACCTATCTCAAGGCCCACCGCACGGCTTCCAGCTTGCGGGTAAAAAAGGGG
GCGCGGGCCGCCTGATTGGACGCACGAAAGGCGGCATGAACACCAAGCTTCATGCCGTAACGGATGCGAGTGGTCGCCCGATCAGCTTCTTCATAACGGC
AGGCCAGGTCAGCGATTACACCGGTGCTGCCGCCTTGCTTGATGAACTTCCCAAGGCCAAATGGTTGCTGGCCGACCGTGGCTATGATGCCGACTGGTAT
CGTGACGCTTTACAGGCGAAGGGGATCACTCCCTGCATTCCAGGTCGAAAATCCCGCAAGAAGACCATCAAATACGACAAACGCCGCTACAAACGGCGCA
ACCGGATCGAGATCATGTTCGGGCGTCTCAAAGACTGGCGGCGTGTCGCTACGCGTTACGACCGGTGCCCAATGGCCTTTCTCTCCGCCATCGCTCTCGC
TGCGACCGTTATCTTCTGGCTATGAGCAATGAGTCCTGACCC
GCTCGTCTTCAGCCCTATTTCCCCAAGAGCCATGGCCGCCAGCGCGTTGATGATCGACGCGTTCTGAGCGGCATCATCTTCGTCAACCGCAACGGGCTGC
GTTGGCGTGATGCGCCGAAGGAATATGGCCCGGCGAAGACGCTTTATAACCGCTGGAAACGCTGGGGCGACAAGGGCGTATTTATCCAGATGATGGAAGG
TCTGGCCGTGCCTGAAGCCGCAGAGCACAAGACCATCATGATCGATGCGACCTATCTCAAGGCCCACCGCACGGCTTCCAGCTTGCGGGTAAAAAAGGGG
GCGCGGGCCGCCTGATTGGACGCACGAAAGGCGGCATGAACACCAAGCTTCATGCCGTAACGGATGCGAGTGGTCGCCCGATCAGCTTCTTCATAACGGC
AGGCCAGGTCAGCGATTACACCGGTGCTGCCGCCTTGCTTGATGAACTTCCCAAGGCCAAATGGTTGCTGGCCGACCGTGGCTATGATGCCGACTGGTAT
CGTGACGCTTTACAGGCGAAGGGGATCACTCCCTGCATTCCAGGTCGAAAATCCCGCAAGAAGACCATCAAATACGACAAACGCCGCTACAAACGGCGCA
ACCGGATCGAGATCATGTTCGGGCGTCTCAAAGACTGGCGGCGTGTCGCTACGCGTTACGACCGGTGCCCAATGGCCTTTCTCTCCGCCATCGCTCTCGC
TGCGACCGTTATCTTCTGGCTATGAGCAATGAGTCCTGACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 351 bp | 116 aa | 65 | 415 | + | No |
Description : First part of the transposase
ORF sequence :
MSNLFWLTDEQMARLQPYFPKSHGRQRVDDRRVLSGIIFVNRNGLRWRDAPKEYGPAKTLYNRWKRWGDKGVFIQMMEGLAVPEAAEHKTIMIDATYLKA
HRTASSLRVKKGARAA
HRTASSLRVKKGARAA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 450 bp | 169 aa | 376 | 825 | + | No |
Description : Second part of the transposase
ORF sequence :
SRRAQDHHDRCDLSQGPPHGFQLAGKKGGAGRLIGRTKGGMNTKLHAVTDASGRPISFFITAGQVSDYTGAAALLDELPKAKWLLADRGYDADWYRDALQ
AKGITPCIPGRKSRKKTIKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPMAFLSAIALAATVIFWL
AKGITPCIPGRKSRKKTIKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPMAFLSAIALAATVIFWL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 761 bp | 253 aa | 65 | 825 | + | Yes |
Chemistry : DDE
ORF sequence :
MSNLFWLTDEQMARLQPYFPKSHGRQRVDDRRVLSGIIFVNRNGLRWRDAPKEYGPAKTLYNRWKRWGDKGVFIQMMEGLAVPEAAEHKTIMIDATYLKA
HRTASSLRVKKGGAGRLIGRTKGGMNTKLHAVTDASGRPISFFITAGQVSDYTGAAALLDELPKAKWLLADRGYDADWYRDALQAKGITPCIPGRKSRKK
TIKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPMAFLSAIALAATVIFWL
HRTASSLRVKKGGAGRLIGRTKGGMNTKLHAVTDASGRPISFFITAGQVSDYTGAAALLDELPKAKWLLADRGYDADWYRDALQAKGITPCIPGRKSRKK
TIKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPMAFLSAIALAATVIFWL
Blast result :
Comments
ISKpn12 is 88% aa similar to ISRhsp4.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Márquez, C., Labbate, M., Raymondo, C., Fernández, J., Gestal, A. M., Holley, M., Borthagaray, Stokes, H. W. (2008). J. Clin. Microbiol. 46 (10), 3417-3425.