ISAfe9

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans ATCC 23270
DNA section
IS Length : 2503 bp

Ends


IR Length : 38/50

IRL : TGCTAAGCGACAATTACCTTGTCCGGCTGACGACAATTATCTTGGCCGGT
IRR : TGCTCGACGTCAATTATCTTGACCCATCGGCGACAATTACCTTGGCCGGT

Insertion site


Left flankDirect repeatRight flankDR Length
CCTTGTTTTCCGAACGACCCATGTGCGACGAAG7

DNA sequence

TGCTAAGCGACAATTACCTTGTCCGGCTGACGACAATTATCTTGGCCGGTAAAACCTGACGCCGGGCAGGAGCCTGAGTGAGGGATAAAGTAGCGTTCTT
GACCTGTAAGGACAGGAGGACGCAATGCCCGGCAAGTGGTTGGGAGATATTCAGGTGAACAAATACAAGGAACTTCGCAGGCAGTATCAGCAAGAGACCG
CTGCCAACAAGATGGGAATTAGTGTGCGCACGGCGCGACGATTAGAAAGGCGGGAGGCTGCTCTGCCCTCCCAGAGGGGGCCACGGACCTGGCGCACGCG
CAAGGATCCCTTTGCGGCCTACTGGCAAAGTGAGATTTTGCCGCTTTTGCGGCAAGCCCCCGGTTTGTTGGCTACGACCTTGCTGGAGGAGATGCAGCGG
TTACATCCGGGAGAGGTAGAGCCTGGCCATCTCCGGACCTTGCAACGCCGCATTCGGGACTGGCGGGCATTGGAGGGGCCGGATCGAGAGATTTACTTCC
CGCAGGCCCACGATCCAGGAAGGCAATGCCTTTCGGATTACACCGACGCCACGGAGTTATGCGTCACTATCGATGGGCAGATTCTGGTCCATAAACTCTA
CCAGTTCGTGATGGCCTACTCGGGGTGGCGCCACGTCGAGGTAGTTCTGGGTGGCGAGAGCTTCATATCCCTTTCTGCAGGTCTACAGAATGCCCTCTGG
CGCCTGGGCGGCGTTCCGCAAGAGCACCGCACGGACCACCTCACGGCGGCCTATAACAATCAATCCGAGCACGAAGAGTTGACCCGGCGGTATGCCGCCC
TGTGTCGGGATTACGATTTGCGGGCCACCACCAATAATCAGGGGGAGTCCCAGGAAAATGGATCGGTTGAGGCCCGTCAACGGACTTTGAAGCAGGCCCT
GAATCAGGCGCTGATGCTGCGGGGTTATCGCGACTTCAGCGATTTGGCCGCATATCAGGCCTTTGTCGAGGAAGTCGCCACCCACATGAATCGGCGGGTG
CAAAAGCGTTTGGCCGAGGAACGCCCGCTGCTCCAGCCTCTTCCCGAGCGACGGAGCAGCGATTACGTCGAGACGACGGTTCGGGTCAGCAGCAATGGGG
TGTTCCGAGTATCCCACACCACGTACAGCGCACCGTCCCGTCTGCGTGGCCATCCTTTGCAGGTACGCATCTACACCCAGCATATCGAAGCATACTATGG
CAGCACTCTGGTGGTGCGTGCGGAGAAAGGCCTGCGGGACGAGGCGGTGATCGATTATCGCCATGTACTGCCCATCCTCCGGCGCAAGCCCGGGGCCCTG
ATGCGTTGGGTATACCGGGATGCCCTGTTCCCTCGCCAGGCTTACCGGGATACCTGGGAGCGCTTACAGGAGCGCTGCCCAGAGCGGGACGCCTGCCGGA
TCATGGTGGGTCTGTTGGAGTTGGCGGCTGATGCCTGTGAGGCGGAGTTGGCATTGCGTTTGGACCAGATCCTGGCGGCGGGAGAACTCCCCGGTCTGGA
GACCCTCCGAATGGCCCTGCATCCCGCACAAACGGTACCGACAGACCCCATCATATCGGTTCCGTCCCTGGAGCAATATGATCAGCTCCTGATGGAGGTG
ACCGCATGAACCCGAGCACGCTCGCCCGACTTCCCATGATGCTCACCGATCTGCGCTTGCCAACCATGAAACAGGCTTGGCAAGGCCTCGCCACAAAGTC
CAATCAGGAAGACTGGCCCGCCGAGCGCTTCCTGGCGACCCTTCTGGAACAGGAAATCCTGGGGCGGGAGGCACGGCGTTTGGAACGTCGTCGCCAGGAA
TCCAACCTGCCACCGGATAAGCGGCTGGGAAACTATGACTTCACGGTGGTTCCCACGCTCTCCAAAGCCCATGTGCAAGCCTTGGCCGAGGCGGATGGAT
GGATCCAGGAAGGGGCCAACCTGCTGCTCTTCGGCCCGCCTGGTGTCGGCAAAAGCCATTTGGTGGCCGCTGTGGGGCATGCCCTAGTGGAACGGGGGTG
TCGGGTATACTTCACACGGACCAGCGATTTGGTTCAGCAGTTACAATCCGCGCGCAAGGAGCTCCGATTGCCGGCCACTCTGGCCAAGCTGGACCGCTTT
GACCTACTGATTTTGGATGACTTCAGCTATGTTCGCCGGGATCAGGCCGAGACCTCGGTGCTCTTCGAACTGATCTCGGAACGCTATGAACGGAAAAGTC
TGGCCATCACAGCCAATCAACCCTTCTCTGCATGGGATCAGGTCTTCCCGGAGGCTGCCATGACCGTGGCCGCCGTGGATCGTCTGGTCCACCGCTCCAC
CATCTTGGAGCTCCATGCAGAAAGCTACCGGCGCCGGACGGCCAAGAATCGCGCAAACGTCACCGTGACAACCAAGGAGGTAAAGCGCGACCTGGAAGAA
ACGGACTCACAGGATGGTGTAGTATCACAGTAACAGCTTAGGGTCCCTCACTCACCGGCCAAGGTAATTGTCGCCGATGGGTCAAGATAATTGACGTCGA
GCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1485 bp494 aa1251609+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPGKWLGDIQVNKYKELRRQYQQETAANKMGISVRTARRLERREAALPSQRGPRTWRTRKDPFAAYWQSEILPLLRQAPGLLATTLLEEMQRLHPGEVEP
GHLRTLQRRIRDWRALEGPDREIYFPQAHDPGRQCLSDYTDATELCVTIDGQILVHKLYQFVMAYSGWRHVEVVLGGESFISLSAGLQNALWRLGGVPQE
HRTDHLTAAYNNQSEHEELTRRYAALCRDYDLRATTNNQGESQENGSVEARQRTLKQALNQALMLRGYRDFSDLAAYQAFVEEVATHMNRRVQKRLAEER
PLLQPLPERRSSDYVETTVRVSSNGVFRVSHTTYSAPSRLRGHPLQVRIYTQHIEAYYGSTLVVRAEKGLRDEAVIDYRHVLPILRRKPGALMRWVYRDA
LFPRQAYRDTWERLQERCPERDACRIMVGLLELAADACEAELALRLDQILAAGELPGLETLRMALHPAQTVPTDPIISVPSLEQYDQLLMEVTA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
828 bp275 aa16062433+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNPSTLARLPMMLTDLRLPTMKQAWQGLATKSNQEDWPAERFLATLLEQEILGREARRLERRRQESNLPPDKRLGNYDFTVVPTLSKAHVQALAEADGWI
QEGANLLLFGPPGVGKSHLVAAVGHALVERGCRVYFTRTSDLVQQLQSARKELRLPATLAKLDRFDLLILDDFSYVRRDQAETSVLFELISERYERKSLA
ITANQPFSAWDQVFPEAAMTVAAVDRLVHRSTILELHAESYRRRTAKNRANVTVTTKEVKRDLEETDSQDGVVSQ

 

Blast result :
Comments
There is 1 copy of ISAfe9 in Acidithiobacillus ferrooxidans ATCC 23270.
ISAfe9 is 61% (ORFA) and 74% (ORFB) aa similar to ISAli13.
References
1] Robert DeBoy (2005) Direct submission.
2] ISfinder annotation (2008)