ISKrh2

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010617 ND Kocuria rhizophila
Kocuria rhizophila DC2201
DNA section
IS Length : 1122 bp

Ends


IR Length : 20/28

IRL : TGTTGTGGGGCATGAGGTTCTTGGCACGAGTGGTGGGGTGATGTGAGGTG
IRR : TGTAGGAGGGCGTGACGTTGTTGACGCGGGCAGGGGTGCGTGAGGCCGGG

Insertion site


Left flankDirect repeatRight flankDR Length
CCATCGGCTACTAGCTAGCTGTACTGACCGGA6
CCAAATCGAGAGTTCTAGGGGCGCTGAAGAAG6

DNA sequence

TGTTGTGGGGCATGAGGTTCTTGGCACGAGTGGTGGGGTGATGTGAGGTGAGCGGGCTCTACCCCGCAGGATGGAAGTTCCTCAACAACCCATCCGCGAG
AAAGCAGAGCCCGCTCATGACTCACGCTAATGCACCCCTGACCCCGACCGGCCGTCTGCGCATGGTGTTGCGCCACCTCGACGAGGGTGTACCCCAAGCC
CACGTAGCCGCGGAGTTCCGGGTCTCCCGACCGACCGTGGCCACCTGGGTTACCCGCTACCGCGCTGAAGGAGAAGCGGGCCTGCACGACCGCACCAGCC
GGCCCAGGTTCCGTCCATCCCAGCTCAACCCTGAGATCATTGCCGAGATCGAATCCCTGCGGCGAGACCGAAAGTGGTCGGCCCGCCGTATCCACCACCA
CTTGCTGGGACTGGGGTACCAGCTGCATCTGCGCACCGTGGGCCGGTGGTTGAGAAGGTTGGGGATCTCCCGGCTGCGTGATCTCACCCCGGACGGGGAG
AACCTGCGCCGGGCACACCAACGCATCCGAGCCCGGTGGGCCGGGCACATGTTGCACTTGGACGTGAAAAAGCTCGGGCGAATCCCTGACGGTGGCGGCT
GGTGGGCCCACGGGCGCGGCAGTATGGACGCTAAAGCGGCCAAGCGTGGGCCCGGGGCGGGGGTCGGGTACACCTACCTGCACTCTGCCGTGGACGGCTT
CTCTCGCCTGGCCTACACCGAGGCCCTCGCCGATGAGAAAGCAGCCACCACGATCGCATTCTTCGCCCGCGCACGGGCGTTCTTCGCCGCCCACGGGATC
ACCCGCATCCACCGGGTGATCACCGACAACGGGGCCAACTACCGGGCGAAGGACTTCACCCGCACCGTGGAAGCACTGGCCAATAGGCATCAGCGGATCC
GCCCCTACACACCGCGACACAACGGCAAAGTCGAGCGCTACAACCGGCTACTGGCCGATGAAGTGCTCTACGCCCGCCCGTATCCCAACGAACAGGCCCG
CCGGGACGCGATCGGGATGTGGGTAAACCACTTCAACTACCATCGGCCTCACACCGCCTGCGGCGACCAGCCCCCGGCCTCACGCACCCCTGCCCGCGTC
AACAACGTCACGCCCTCCTACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1011 bp336 aa1171127+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTHANAPLTPTGRLRMVLRHLDEGVPQAHVAAEFRVSRPTVATWVTRYRAEGEAGLHDRTSRPRFRPSQLNPEIIAEIESLRRDRKWSARRIHHHLLGLG
YQLHLRTVGRWLRRLGISRLRDLTPDGENLRRAHQRIRARWAGHMLHLDVKKLGRIPDGGGWWAHGRGSMDAKAAKRGPGAGVGYTYLHSAVDGFSRLAY
TEALADEKAATTIAFFARARAFFAAHGITRIHRVITDNGANYRAKDFTRTVEALANRHQRIRPYTPRHNGKVERYNRLLADEVLYARPYPNEQARRDAIG
MWVNHFNYHRPHTACGDQPPASRTPARVNNVTPSYS

 

Blast result :
Comments
ISKrh2 is 69% aa similar to ISRae1. The codon STOP is in the insertion site.
References
1] Takarada,H., Sekine,M., Kosugi,H., Matsuo,Y., Fujisawa,T., Omata,S., Kishi,E., Shimizu,A., Tsukatani,N., Tanikawa,S., Fujita,N. and Harayama,S.(2008) J. Bacteriol. 190 (12), 4139-4146
2] ISfinder annotation (2008)