ISKrh3

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010617 ND Kocuria rhizophila
Kocuria rhizophila DC2201
DNA section
IS Length : 1113 bp

Ends


IR Length : 20/27

IRL : TGTTGTGGGTCATGACGTTGTAGTCACCGATCAGGGATGAAGGAACGGGC
IRR : TGTATGAGGTCATGACGTTGTCTACACGTTCGTGGACGCGGCTGGCCGGT

Insertion site


Left flankDirect repeatRight flankDR Length
AATTTATGTCGGCCCTAGACAGATCTAAGCC6

DNA sequence

TGTTGTGGGTCATGACGTTGTAGTCACCGATCAGGGATGAAGGAACGGGCCCCTGGACTGCACGATGGGTGGTGTCGAAGCAACCATCCAGCAGAAAGAG
ACCCGTTCCGATGGCTCACGCTAACGCCCCGCTGACCCCGGAAGGAAGACAGCGCCTCGTCACCCGCGTCCTCGACGATGACCGGCCCATCTCCCACGTC
GCCACTGAAGCCGGCATCGCGCGTTCCACACTGACCAAATGGGTGGCTCGCTACCGGGCCGACGGCCCCGAAGGACTCGGGGACCGCTCCAGCGCACCAG
TTGAGCGCCCGACCCGGGTATCTCTCGAGGTGCTCGAGCTGATCGACCGGTGGCGTCGCGACCACAAGTGGTCGGCTCGGCGTATCCATCTCGAACTGAC
CGAACGCGGTCATCACTGCTGCATGCGCACGGTATCCCGCTGGTTGAAGCGGCTCGGGATCAGCCGACGTCGTGACCTGGACCCGACCGGAGAGAACAAC
CGTACAGTCGGGAAGATCACCGCCCGGTTCCCCGGCCACATGCTCCACCTGGACGTGAAGAAAGTCGGGAAGATCCCTGACGGGGGCGGCTGGTGGGCTC
ATGGACGCGGCAGCAGCAAAGCACTGGCATCCAAGCGCGCGCATAAGCAGCGCGTCGGCTACACCTACTTGCACTCGGCAGTCGATGGATACTCCCGGCT
GGCGTACACCGAAGCACTCGAAGACGAGACCGCCCAGGCCACGATCGGGTTCTTCGCCCGGGCTCGCGCATACTTCGCAGCCCACGGCATCACGCGCCTC
ATTCGCGTCGTGACGGACAACGGGGCCAACTATCGAGCGGCACGATTCGTGGCCGCTGTGCAGTCGCATGCCTCACGGCATCAGCGGACACGGGCGTACA
CGCCGAGGCATAACGGGAAGGTCGAGCGGTACCAGAGGCTGCCTTCCGAGGAATGCCTCTATGCCCGCGTCTACGACTCCGAACAGGCTCGTCGGAAGGC
GATCGGGGTGTGGGTCCATCACTACAACTATCATCGGCCTCATACCGCCTGTGCAGATCAGCCACCGGCCAGCCGCGTCCACGAACGTGTAGACAACGTC
ATGACCTCATACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1008 bp335 aa1111118+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAHANAPLTPEGRQRLVTRVLDDDRPISHVATEAGIARSTLTKWVARYRADGPEGLGDRSSAPVERPTRVSLEVLELIDRWRRDHKWSARRIHLELTERG
HHCCMRTVSRWLKRLGISRRRDLDPTGENNRTVGKITARFPGHMLHLDVKKVGKIPDGGGWWAHGRGSSKALASKRAHKQRVGYTYLHSAVDGYSRLAYT
EALEDETAQATIGFFARARAYFAAHGITRLIRVVTDNGANYRAARFVAAVQSHASRHQRTRAYTPRHNGKVERYQRLPSEECLYARVYDSEQARRKAIGV
WVHHYNYHRPHTACADQPPASRVHERVDNVMTSYT

 

Blast result :
Comments
ISKrh3 is 68% aa similar to ISNfa1. The STOP codon is in the insertion site.
References
1] Takarada,H., Sekine,M., Kosugi,H., Matsuo,Y., Fujisawa,T., Omata,S., Kishi,E., Shimizu,A., Tsukatani,N., Tanikawa,S., Fujita,N. and Harayama,S.(2008) J. Bacteriol. 190 (12), 4139-4146
2] ISfinder annotation (2008)