ISKpn11
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| EU780013 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae 12836 |
DNA section
IS Length : 1290 bp
Ends
IR Length : 16
IRL : ATCAGAGGTGGCTCGGGAAATCTGCACAGCGGTGATAAGTGGAATTTCTG
IRR : ATCAGAGGTGGCTCGGTTTGTCTGAACAGTTTTGGGCGTTTTGCTAAGTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCTCGTGCACCCGGACT | CAGCAACAGA | 0 |
DNA sequence
ATCAGAGGTGGCTCGGGAAATCTGCACAGCGGTGATAAGTGGAATTTCTGCCTGACTTCGGCTTAGATGCCGGGAACAGGAGATACCATGACAAGACGAC
CGCGCCGGAACCACAGCCCGGCTTTCAAGGCAAAAGTGGCGCTCGCCGCGATCCGAGGTGAGCAGACGCTGGTGGAATTGTCCCAGCAGTTCGACGTGCA
TGCCAACCAGATCAAACAGTGGAAAGACCAGCTCCTTGATGGGGCGACAGGCGTTTTCGGCGATGAAGCGAAGGCGGAACCGGCAGGTCCAACCGTCGAT
GTCAAAACGCTGCACGCCAAAATAGGCGAACTGACATTGGAGAATGATTTTTTAGCCGGAGCGCTCGGCAAAGCGGGATTGCTGGGCGGAAAGAAATGAT
CGACCGAACACACAAACTGTCGGTCGCACGTCAGGCCAGGCTTCTCGGCTTCAGCCGTGGCAGTGTCTACTATTCTCCACGTCAAGTGTCCGACGGCGAT
CTGGACCTGATGCGGCGGATCGACGAACTGCACCTCGACTATCCGTTCGCCGGAAGTCGGATGTTGCAAGGGCTTTTGAGGGGTGAAGGGACCGAGGTCG
GGCGGTTGCACGTCGCCACGCTGATGAAGAAGATGGGCATCGAGGCAATCTACCGTCGCCCGAACACCTCCAAACCAGCGCCAGGTCACAAAATCTATCC
TTATCTCCTGCGCAAGCTGGCGGTCACCCGGCCCAACCAGGTCTGGGCAATGGACATAACGTATGTGCCGATGGCGCGGGGATTCGTCTATCTCTGCGCC
GTCGTGGACTGGTTTAGCCGGCGGGTTCTGTCCTGGCGGCTGTCGATCACGATGGAGGCGGACTTCTGCATCGAAGCGGTCGAGGACGCGCTGGCCCGCT
ATGGAAAGCCTGAAATATTCAACACCGATCAGGGTTCGCAGTTCACCTCCATCGACTTCACAGCCGTGCTGAAGAAGGCGGAGATCGCCATCTCGATGGA
TGGCAAGGGCGCGTGGCGCGACAACGTCTTCGTCGAGCGGCTCTGGCGGTCGATCAAATACGAAGAGGTCTATCTCCACGCCTACAAGACCGTATCCGAG
GCTCGCGCTGGCATCGGCCGCTATCTCGCCTTCTACAATAGCCGACGTCCACATTCATCCCTTGACCGGCAGACGCCGGATCAGGCCTACTTCAACGCGC
TGGCACCAATGATGGTGGCGGCATAATCAAGGCGGAAATCCACTTAGCAAAACGCCCAAAACTGTTCAGACAAACCGAGCCACCTCTGAT
CGCGCCGGAACCACAGCCCGGCTTTCAAGGCAAAAGTGGCGCTCGCCGCGATCCGAGGTGAGCAGACGCTGGTGGAATTGTCCCAGCAGTTCGACGTGCA
TGCCAACCAGATCAAACAGTGGAAAGACCAGCTCCTTGATGGGGCGACAGGCGTTTTCGGCGATGAAGCGAAGGCGGAACCGGCAGGTCCAACCGTCGAT
GTCAAAACGCTGCACGCCAAAATAGGCGAACTGACATTGGAGAATGATTTTTTAGCCGGAGCGCTCGGCAAAGCGGGATTGCTGGGCGGAAAGAAATGAT
CGACCGAACACACAAACTGTCGGTCGCACGTCAGGCCAGGCTTCTCGGCTTCAGCCGTGGCAGTGTCTACTATTCTCCACGTCAAGTGTCCGACGGCGAT
CTGGACCTGATGCGGCGGATCGACGAACTGCACCTCGACTATCCGTTCGCCGGAAGTCGGATGTTGCAAGGGCTTTTGAGGGGTGAAGGGACCGAGGTCG
GGCGGTTGCACGTCGCCACGCTGATGAAGAAGATGGGCATCGAGGCAATCTACCGTCGCCCGAACACCTCCAAACCAGCGCCAGGTCACAAAATCTATCC
TTATCTCCTGCGCAAGCTGGCGGTCACCCGGCCCAACCAGGTCTGGGCAATGGACATAACGTATGTGCCGATGGCGCGGGGATTCGTCTATCTCTGCGCC
GTCGTGGACTGGTTTAGCCGGCGGGTTCTGTCCTGGCGGCTGTCGATCACGATGGAGGCGGACTTCTGCATCGAAGCGGTCGAGGACGCGCTGGCCCGCT
ATGGAAAGCCTGAAATATTCAACACCGATCAGGGTTCGCAGTTCACCTCCATCGACTTCACAGCCGTGCTGAAGAAGGCGGAGATCGCCATCTCGATGGA
TGGCAAGGGCGCGTGGCGCGACAACGTCTTCGTCGAGCGGCTCTGGCGGTCGATCAAATACGAAGAGGTCTATCTCCACGCCTACAAGACCGTATCCGAG
GCTCGCGCTGGCATCGGCCGCTATCTCGCCTTCTACAATAGCCGACGTCCACATTCATCCCTTGACCGGCAGACGCCGGATCAGGCCTACTTCAACGCGC
TGGCACCAATGATGGTGGCGGCATAATCAAGGCGGAAATCCACTTAGCAAAACGCCCAAAACTGTTCAGACAAACCGAGCCACCTCTGAT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 333 bp | 109 aa | 67 | 399 | + | No |
Description : First part of the transposase
ORF sequence :
MPGTGDTMTRRPRRNHSPAFKAKVALAAIRGEQTLVELSQQFDVHANQIKQWKDQLLDGATGVFGDEAKAEPAGPTVDVKTLHAKIGELTLENDFLAGAL
GKAGLLGGK
GKAGLLGGK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 879 bp | 292 aa | 348 | 1226 | + | No |
Description : Second part of the transposase
ORF sequence :
FFSRSARQSGIAGRKEMIDRTHKLSVARQARLLGFSRGSVYYSPRQVSDGDLDLMRRIDELHLDYPFAGSRMLQGLLRGEGTEVGRLHVATLMKKMGIEA
IYRRPNTSKPAPGHKIYPYLLRKLAVTRPNQVWAMDITYVPMARGFVYLCAVVDWFSRRVLSWRLSITMEADFCIEAVEDALARYGKPEIFNTDQGSQFT
SIDFTAVLKKAEIAISMDGKGAWRDNVFVERLWRSIKYEEVYLHAYKTVSEARAGIGRYLAFYNSRRPHSSLDRQTPDQAYFNALAPMMVAA
IYRRPNTSKPAPGHKIYPYLLRKLAVTRPNQVWAMDITYVPMARGFVYLCAVVDWFSRRVLSWRLSITMEADFCIEAVEDALARYGKPEIFNTDQGSQFT
SIDFTAVLKKAEIAISMDGKGAWRDNVFVERLWRSIKYEEVYLHAYKTVSEARAGIGRYLAFYNSRRPHSSLDRQTPDQAYFNALAPMMVAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1160 bp | 386 aa | 67 | 1226 | + | Yes |
Chemistry : DDE
ORF sequence :
MPGTGDTMTRRPRRNHSPAFKAKVALAAIRGEQTLVELSQQFDVHANQIKQWKDQLLDGATGVFGDEAKAEPAGPTVDVKTLHAKIGELTLENDFFSRSA
RQSGIAGRKEMIDRTHKLSVARQARLLGFSRGSVYYSPRQVSDGDLDLMRRIDELHLDYPFAGSRMLQGLLRGEGTEVGRLHVATLMKKMGIEAIYRRPN
TSKPAPGHKIYPYLLRKLAVTRPNQVWAMDITYVPMARGFVYLCAVVDWFSRRVLSWRLSITMEADFCIEAVEDALARYGKPEIFNTDQGSQFTSIDFTA
VLKKAEIAISMDGKGAWRDNVFVERLWRSIKYEEVYLHAYKTVSEARAGIGRYLAFYNSRRPHSSLDRQTPDQAYFNALAPMMVAA
RQSGIAGRKEMIDRTHKLSVARQARLLGFSRGSVYYSPRQVSDGDLDLMRRIDELHLDYPFAGSRMLQGLLRGEGTEVGRLHVATLMKKMGIEAIYRRPN
TSKPAPGHKIYPYLLRKLAVTRPNQVWAMDITYVPMARGFVYLCAVVDWFSRRVLSWRLSITMEADFCIEAVEDALARYGKPEIFNTDQGSQFTSIDFTA
VLKKAEIAISMDGKGAWRDNVFVERLWRSIKYEEVYLHAYKTVSEARAGIGRYLAFYNSRRPHSSLDRQTPDQAYFNALAPMMVAA
Blast result :
Comments
ISKpn11 is 61%(ORFA) and 71%(ORFB) aa similar to ISAli5.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Ends are not typical of the family.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Ends are not typical of the family.
References
1] Márquez, C., Labbate, M., Raymondo, C., Fernández, J., Gestal, A. M., Holley, M., Borthagaray, Stokes, H. W. (2008). J. Clin. Microbiol. 46 (10), 3417-3425