ISVei5
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008786 | ND | Verminephrobacter eiseniae | Verminephrobacter eiseniae EF01-2 |
DNA section
IS Length : 1620 bp
Ends
IR Length : 13/16
IRL : GTCGGCCCTGCAAATTCTCTGCGACCCGCATGAATGCTGGATTTTTGACT
IRR : GTCGGGCCTGCGAAATTCATTTCGCTATCGCAGCGAACCGCGCGGCGGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGTGTTCGCCACC | TA | CCTCGGCACGGTG | 2 |
| CCGCCTCC | CACGTA | CAGGGCATAACCCC | 6 |
| GCTTGCCAATACGC | TCGGTA | TGGGCTGCGCGATG | 6 |
| GGCCCGCATCACCG | TATTGA | GGGTTGCCTCGGTG | 6 |
| CGAGACTGGAGCCA | TGGATA | TGGTGCAACACCTG | 6 |
DNA sequence
GTCGGCCCTGCAAATTCTCTGCGACCCGCATGAATGCTGGATTTTTGACTCTTTGTGGTCCACCGAATCTCCCAGGATTCGTTGAACAGGCAGCTGATTC
CTGAGAGATTTGCGCAATGCCCGAGCCCACCGACACGGCTGATTTCTTCCGCTCCCGTCTGGCCGAGATGATCGACTTGCGCCACCCGCTGGCGGTGCTG
GCGAGCCGTCTTCCCTGGACGCAGATCGAAGCGGCACTGGCACCTTATTTCGCGCGTCGGGTCCGTGCCGGTCGAGTTCTGGAACACGACGATCTCTTCG
GCCCTTCGCTCCAGGTCGCGGGCGCCGGTGTCGCCGCCGCGGGCCGCCCGCGTCTTCCCCTGCGCCTCATGGCCAGCCTGCTGTATCTGAAGCACGTCTA
CAAGCTCAGCGACGAGGAACTGGTCGAGCGCTGGGCCGAGAACGTGCAGTGGCAGTTCTTCAGCGGCATGACCTTCTACGAGCCACGCCTGCCCTGCGAC
GCCACCCAGATCGGGCGCTTCCGCTCGATCCTGGGCGAGGCCGGTGTCGAGGAACTCCTGAAGGCCACCATCGACACTGCGGTGGCCTCCAAGGCGATCA
AGCCGGCCGAGTTCGAGCGCGTGATCGTGGATACCACCGTCCAGGAGAAGGCCATCGCCCACCCCGTGGACTCCCGCCTGCTGGAGATCGCCCGCGCCAA
GGTGGTGGCTGCCGCCAAGCGCGCGGGCATCGCGCTGAAGCAGACCTTCGCCAAGGAAGGCAAGAACCTGCGCCGCAGGGCTGGCGGCTACGCCCACGCC
AAGCAGTTCAAGCGCTTGAAGAAGGTGCTCAAGCGCCAGCGCACGGTACTGGGTATCGTGCTGCGCGAAGTGCAGCGCAAGCTTTGCAAGTTGCCTGCGA
CATCGGTGCCTGCCGTCGATCGCTTGCGCGCACTGATGGGCCGCGCCGAGCGCATTCGCACCCAGCGCCCGAAGGACAAGAACAAACTCTATGCATTGCA
CGCGCCCGAAGTTGAGTGCATCGGCAAGGGGAAGGCGCGCCGGCCTTACGAGTTTGGCGTGAAGGTCAGCGTGGCCGTCACGCACAAGCAGGGGCTGGTG
GTCGGTGCGCGCAGCTTCACGGGCAACCCGTATGACGGCCACACGCTGGCCGAGCAACTCGAGCAGGTCAAGATCCTGACGGAGGACACGGGCGCCAGCC
CGAAGCAGGCAGTGGTGGACCTGGGGTTCCGGGGCGTGGACGCAGCCAATCCGGGCATCCAGATCATTCATCGGGGGCGCTTCAAGTCCTTGACCGATGC
CCAGCGCCGCTGGCTCAAGCGAAGGCAGGCGGTGGAGCCCACCATCGGACACCTGAAGCACGACAACGGGATGGATCGGTGCTGGCTGCAGGGTGCCACC
GGTGACGCGCTGCATGCAGTGCTGTGTGCGGCGGGCTACAACATCCGCTGGCTGCTGCGCGCCATCATGCGCCTCGGGCTCAAGGGCCTTTTATTGCGCC
TGATCGCCTTGCTGCGCACGCTCATCGGCGTCCCCCACTTCTTCGCATCCAACGAAATCACGCCATCACGCGCCGCCGCGCGGTTCGCTGCGATAGCGAA
ATGAATTTCGCAGGCCCGAC
CTGAGAGATTTGCGCAATGCCCGAGCCCACCGACACGGCTGATTTCTTCCGCTCCCGTCTGGCCGAGATGATCGACTTGCGCCACCCGCTGGCGGTGCTG
GCGAGCCGTCTTCCCTGGACGCAGATCGAAGCGGCACTGGCACCTTATTTCGCGCGTCGGGTCCGTGCCGGTCGAGTTCTGGAACACGACGATCTCTTCG
GCCCTTCGCTCCAGGTCGCGGGCGCCGGTGTCGCCGCCGCGGGCCGCCCGCGTCTTCCCCTGCGCCTCATGGCCAGCCTGCTGTATCTGAAGCACGTCTA
CAAGCTCAGCGACGAGGAACTGGTCGAGCGCTGGGCCGAGAACGTGCAGTGGCAGTTCTTCAGCGGCATGACCTTCTACGAGCCACGCCTGCCCTGCGAC
GCCACCCAGATCGGGCGCTTCCGCTCGATCCTGGGCGAGGCCGGTGTCGAGGAACTCCTGAAGGCCACCATCGACACTGCGGTGGCCTCCAAGGCGATCA
AGCCGGCCGAGTTCGAGCGCGTGATCGTGGATACCACCGTCCAGGAGAAGGCCATCGCCCACCCCGTGGACTCCCGCCTGCTGGAGATCGCCCGCGCCAA
GGTGGTGGCTGCCGCCAAGCGCGCGGGCATCGCGCTGAAGCAGACCTTCGCCAAGGAAGGCAAGAACCTGCGCCGCAGGGCTGGCGGCTACGCCCACGCC
AAGCAGTTCAAGCGCTTGAAGAAGGTGCTCAAGCGCCAGCGCACGGTACTGGGTATCGTGCTGCGCGAAGTGCAGCGCAAGCTTTGCAAGTTGCCTGCGA
CATCGGTGCCTGCCGTCGATCGCTTGCGCGCACTGATGGGCCGCGCCGAGCGCATTCGCACCCAGCGCCCGAAGGACAAGAACAAACTCTATGCATTGCA
CGCGCCCGAAGTTGAGTGCATCGGCAAGGGGAAGGCGCGCCGGCCTTACGAGTTTGGCGTGAAGGTCAGCGTGGCCGTCACGCACAAGCAGGGGCTGGTG
GTCGGTGCGCGCAGCTTCACGGGCAACCCGTATGACGGCCACACGCTGGCCGAGCAACTCGAGCAGGTCAAGATCCTGACGGAGGACACGGGCGCCAGCC
CGAAGCAGGCAGTGGTGGACCTGGGGTTCCGGGGCGTGGACGCAGCCAATCCGGGCATCCAGATCATTCATCGGGGGCGCTTCAAGTCCTTGACCGATGC
CCAGCGCCGCTGGCTCAAGCGAAGGCAGGCGGTGGAGCCCACCATCGGACACCTGAAGCACGACAACGGGATGGATCGGTGCTGGCTGCAGGGTGCCACC
GGTGACGCGCTGCATGCAGTGCTGTGTGCGGCGGGCTACAACATCCGCTGGCTGCTGCGCGCCATCATGCGCCTCGGGCTCAAGGGCCTTTTATTGCGCC
TGATCGCCTTGCTGCGCACGCTCATCGGCGTCCCCCACTTCTTCGCATCCAACGAAATCACGCCATCACGCGCCGCCGCGCGGTTCGCTGCGATAGCGAA
ATGAATTTCGCAGGCCCGAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1488 bp | 495 aa | 117 | 1604 | + | No |
Chemistry : DDE
ORF sequence :
MPEPTDTADFFRSRLAEMIDLRHPLAVLASRLPWTQIEAALAPYFARRVRAGRVLEHDDLFGPSLQVAGAGVAAAGRPRLPLRLMASLLYLKHVYKLSDE
ELVERWAENVQWQFFSGMTFYEPRLPCDATQIGRFRSILGEAGVEELLKATIDTAVASKAIKPAEFERVIVDTTVQEKAIAHPVDSRLLEIARAKVVAAA
KRAGIALKQTFAKEGKNLRRRAGGYAHAKQFKRLKKVLKRQRTVLGIVLREVQRKLCKLPATSVPAVDRLRALMGRAERIRTQRPKDKNKLYALHAPEVE
CIGKGKARRPYEFGVKVSVAVTHKQGLVVGARSFTGNPYDGHTLAEQLEQVKILTEDTGASPKQAVVDLGFRGVDAANPGIQIIHRGRFKSLTDAQRRWL
KRRQAVEPTIGHLKHDNGMDRCWLQGATGDALHAVLCAAGYNIRWLLRAIMRLGLKGLLLRLIALLRTLIGVPHFFASNEITPSRAAARFAAIAK
ELVERWAENVQWQFFSGMTFYEPRLPCDATQIGRFRSILGEAGVEELLKATIDTAVASKAIKPAEFERVIVDTTVQEKAIAHPVDSRLLEIARAKVVAAA
KRAGIALKQTFAKEGKNLRRRAGGYAHAKQFKRLKKVLKRQRTVLGIVLREVQRKLCKLPATSVPAVDRLRALMGRAERIRTQRPKDKNKLYALHAPEVE
CIGKGKARRPYEFGVKVSVAVTHKQGLVVGARSFTGNPYDGHTLAEQLEQVKILTEDTGASPKQAVVDLGFRGVDAANPGIQIIHRGRFKSLTDAQRRWL
KRRQAVEPTIGHLKHDNGMDRCWLQGATGDALHAVLCAAGYNIRWLLRAIMRLGLKGLLLRLIALLRTLIGVPHFFASNEITPSRAAARFAAIAK
Blast result :
Comments
The genome of V. eiseniae EF01-2 contains 5 copies of ISVei5. ISVei5 is 71% aa similar to IS1478.
References
1] Pinel, N., Kjeldsen, K.U., Lund M.B., Davidson S.K.,..., and Stahl D.A. (2008) Comparative analysis of the genome sequences of Verminephrobacter eiseniae, Acidovorax avenae subsp. citrulli, and Acidovorax sp. JS42. (in preparation)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Stahl,D. and Richardson,P. (2007) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Stahl,D. and Richardson,P. (2007) Direct submission GenBank.