ISfinder

ISAzo17

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006513	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 Azoarcus sp. EbN1 plasmid 2

DNA section

IS Length : 2095 bp

Ends

IR Length : 13/18

IRL : TGTCACTGCCGGATGTAAGTTGATACGGGCTGCCGATTGAAAGTTGATAC
IRR : TGTCAATGCCGATTTAAAACTGACACGCTTTCCCGGCGGTTGCCGATTTA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCCCACGCCC	CACCT	AGGCGTGGAA	5
CGAACGGCAT	GGACT	CGGCCGAGCA	5
GCCGCACTTC	TGGAT	GGTTTCAATC	5
CGATTCGCGC	ATCGT	CGCGCTCGAC	5
GCCCGCATCC	CATCA	GGCGCATCGC	5
GCCTAGGCCA	AGCAC	CCGCTGCAAG	5
CGGGGTGGTG	ATCCG	CGATGGCTAT	5
AACGGCGGCG	GCAAG	ATCGTAAATA	5
GCCACGGGCG	AAGC	GACCAGTGGC	4

DNA sequence

TGTCACTGCCGGATGTAAGTTGATACGGGCTGCCGATTGAAAGTTGATACACCGAGTTGAGAAGATCGGGCCATTTGGAGCCCGGGTGATCACAGACGAG
GTGTATGTGGAAATCGAACTGTTGAGGCGTCACGGGTTGAGCCTTCGGCGGATCGCCGCCGAGGTGGGGTGTGCGGTGAACACGGTGCGTGCGCACCTGG
CGTCGCCGGAGCTGCCACGCTACGCGCGCAAGGTCCAGCGGGTGACGAAGCTGGCGCCCTTCGAGGCGTACTTGCGCGAACGCCAGGCGGCGGCGCATCC
GCACTGGATTCCGGCCTCGGTCCTGATGCGTGAGATCGTGGCGCAGGGCTATCAGGGTGGCGCCAGCCAGCTGCGCGCGTTCATGCACATGCTCAAGCCG
GCGCAGCCATCGGAGCCGGTGGTGCGCTTCGAGACGGCGCCGGGCCACCAGATGCAGGTCGACTGGGTGGAGTTTCGCAAGGGCGCGCAGCCGCTGTACG
CGTTCTGCGCCACGCTCGGCTACAGCCGCATGAGCTACGTCGAGTTTGTCACCGACATGAAGGTGACGACGCTGATCGGCTGCCACGAGCGCAGCTTCGC
GGCGTTCGGGGGCGTGGTGCGCCAGGTGCTGTACGACAACATGAAGACCGTGGTGCTCGAGCGCGATGTCGACGGCGAAGGCGCGCACCGCTACCACGCC
GGCTTTCTGGACTACGCCCGTCACGCCGGGTTCGTGATCAAGCTGTGCCGCCCCTATCGGGCACGGACCAAGGGCAAGGTGGAGCGCTTCAACGGCTATC
TGCGGCGCTCGTTCTACGTGCCGCTGGTGGCGCAGTTCAAGCAGGCCGGTCTGGTGCTCGACGCCGCCACGGCCACGGTGCAGGTGCGCCGCTGGCTCGA
CGAAGTCGCCAACGTGCGCGTGCATGGCACCACCGGCGAGCAACCGGTAGCGCGGCTGGCGGCCGAACGGGCTGCGCTGCAGGCGCTGGCACCGCCGTGG
CGGGGCGACATCGAGGGCGCCCGGCCGCAGGCTGAGGCGGCACACGACGAGGGCCCCGTGCGCCCGCCCGCCGTGCGTGCGCACCTCGAAACGGCGCAAC
CCGCCCAGCATCCGCTGGCCGTCTACGACGCGTTGCTGCAGACGCTGCAACAGGCGCAGGAGATCGGCGCATGAACCTGCAATACGAACGGATCCAGGCG
CTGTGCCAGACGCTGAGCCTGCCGCTCACCGCCCAAGGCTATGCCGCAGCGGCCCAGCAGGCGGCCACCGATCAACTGGCCTACAGCGACTTCCTCGAAC
AGTTGCTGCGGGCCGAGGCGGCCGGTCGTCAGAGCCGCAAGCAAAGCATGCTCACGCGCCTGGCGGGCTTCCCCGCGATCAAGACGCTGGAGGATTTCGA
CTATGGCTTTGCCAGCGGGCTCAAGCGCAGCCAGATCGAGGAGTTGGCCAGCCTCGCCTTCGTCGAGCGTGCCGAGAACGTTGTGCTGGTGGGCCCCAGC
GGGGTGGGCAAGACCCATCTGGCCATCGCGCTGGGCTACCGCGCCACGCAGGCCGGCATCAAGACGCGCTTCACCACCGCCGCCGACCTGCTGCTGACGC
TGGTCCTGGCGCATGAGCGCAACCAGCTCAAGAACGTGATGCAGCGGGCGATCAACGCCTACCGGCTGTTGATCGTCGACGAGATCGGCTACCTGCCGAT
GACGCGCGAGCAGGCCAACCTGTTCTTCCAGGTGATCGCGGCGCGCTACGAGCGGGGCAGCCTGATCGTGACCAGCAATCTGTCGTTCGGGCAGTGGGAC
AGCACCTTCGCCCAGGATGCGACGCTCACGGCGGCGCTACTGGATCGGCTGCTGCATCACGCGCACATCGTGCCGATCAGCGGCGAGAGCTACCGGCTGA
AACACCAGCGCAAGGCCGGGATGGTGCAAGCGCTGGCGGTCGCCGGCTGAGGGCCGCGCGACGGAGCGCCCCTGCGGTCGGGCCTTCGGCCCTCCCTTCG
GGGCGCTCCGTCGCACATGTCAATGGGATAGAGGTGTATCAGTTTTAAATCGGCAACCGCCGGGAAAGCGTGTCAGTTTTAAATCGGCATTGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1089 bp	362 aa	86	1174	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VITDEVYVEIELLRRHGLSLRRIAAEVGCAVNTVRAHLASPELPRYARKVQRVTKLAPFEAYLRERQAAAHPHWIPASVLMREIVAQGYQGGASQLRAFM
HMLKPAQPSEPVVRFETAPGHQMQVDWVEFRKGAQPLYAFCATLGYSRMSYVEFVTDMKVTTLIGCHERSFAAFGGVVRQVLYDNMKTVVLERDVDGEGA
HRYHAGFLDYARHAGFVIKLCRPYRARTKGKVERFNGYLRRSFYVPLVAQFKQAGLVLDAATATVQVRRWLDEVANVRVHGTTGEQPVARLAAERAALQA
LAPPWRGDIEGARPQAEAAHDEGPVRPPAVRAHLETAQPAQHPLAVYDALLQTLQQAQEIGA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
780 bp	259 aa	1171	1950	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNLQYERIQALCQTLSLPLTAQGYAAAAQQAATDQLAYSDFLEQLLRAEAAGRQSRKQSMLTRLAGFPAIKTLEDFDYGFASGLKRSQIEELASLAFVER
AENVVLVGPSGVGKTHLAIALGYRATQAGIKTRFTTAADLLLTLVLAHERNQLKNVMQRAINAYRLLIVDEIGYLPMTREQANLFFQVIAARYERGSLIV
TSNLSFGQWDSTFAQDATLTAALLDRLLHHAHIVPISGESYRLKHQRKAGMVQALAVAG

Blast result :

Comments

ISAzo17 is 80% (ORFA) and 90%(ORFB) aa similar to ISCARN3.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).