ISfinder

ISAcma16

Family IS4
Group IS4

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009925	ND	Acaryochloris marina	Acaryochloris marina MBIC11017

DNA section

IS Length : 1483 bp

Ends

IR Length : 18

IRL : CAATGGCACTGACTTAAGAAGTCAGGAAAAAGCTACAAGTCTTGATAGAT
IRR : CAATGGCACTGACTTAAGCTCAATCAAACAGCTAGCTTAGCTTTGAGAGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCAGATCAATCACATCCCCC	ACCCCCAGAT	AGCGCACCTGGCTCGCCCTC	10
AGCAATGAGGTATTGACT	ATCCCTTGGAAT	ATCTTTCTCCTTGACAAC	12
CTACCATTGGGTCGATGGCT	AGCTATAGTT	TATTCGCTCTTTCTAGCCAG	10
AAATATTGAAAGGGAGAAAG	CCCCATAGAT	TGCTTGAACAGACGGCTAAA	10
AGAAGGCGAATCTATCGCT	ATTGCATCGTT	CAATCTCTCAACGCTGAAT	11
GTCGGTATTTCCAGATACG	TCCCTATGGGT	TGCCATCCATAGGGAATAC	11
CCCAATGTTCAAGAGAGCT	ATCCCAACAGT	GGTTGTTCAAGAATGAACT	11
CTCAATTCCCTGCCTGATCA	GTCTATAGAT	GCTTTGAAAATTCAGTAATT	10
GTTTGCTGAGTCGTTTAAGC	AGTCCTGATT	CGCCCAGAATATCTTCCGGT	10
ATCACCGCCTTGGGATAAAC	TATTAAAGTT	GGCTTGACCTGAATCGGGCA	10
GGGAAATCCTTTCCCAAGTT	ATTTATGGAT	TCCTGCAGTTTTTGCCCAAA	10
AGTTCATTCTTGAACAACC	ACTGTTGGGAT	AGCTCTCTTGAACATTGGG	11
AGGAAACGCTGCATCTGTC	ATCTAACGGAT	AGGTCTCAGGGGTGCGAGA	11

DNA sequence

CAATGGCACTGACTTAAGAAGTCAGGAAAAAGCTACAAGTCTTGATAGATATAGGATCAAGACTTGCACGCCATAAACTTCTGCCTTTCACTATGTCTGA
GTCAGCCGAAATTCTCAAGCAGCAATTTTCCCAAAGCCTGGGTTTACCGTGGACAGATATCCTTCCAGCCTCAAGGTTAGAGGAACTCCTGAAAGAAGAA
GCATTCTCCTATCGCAACCGGATATATAGCCCCATCGTTACGCTGTGGGCCATGCTTTACCAAGTGCTATCAGCCGATAAAAGCCTTCGTAACACAGTCA
AATGCATCACGACTTGGCTCACAGCGGCTGGCATCCAACCGCCCTCATCTGATACCGGAGCCTACAGCAAAGCCAGAAGTCGCTTTCCAGAGTCACTGTT
GCAACGTTTAATTCCCGAAAGCGCTGAGTGCTTAGCGCAACCCCTCTCCCCAGAGCACCTCTGGTGTGGTCGGCCCGTCAAGGTCTACGACGGAACCACC
GTGTTGATGGCTGATAGTGCGGCCAACCAAGCATCATATCCACAACATGGCAATCAAACAGCAGGCTGTGGTTTTCCCATCGCTCGCTTGGTAGTGTTCT
TCTGTTTGGTTACCGGTGCAGTGGCGTCAGCTTGTATTGCCTCCTGGGACACCAGTGAAATTGTCATGAGTCGTTTGCTCTATCAAGACCTTGAGGTCGG
TGATGTGGTCATGGCGGACCAAGCTTATGGCAGCTATGTTGATCTAGCCATCATTCAACAACACAGGGCTGATGGAGTGTTGCGTAAACATCATGCTCGC
AAGACTGATTTCCGCAAAGGCAACAAGCATGGCATTGGTGATCATCAGGTGACATGGCATAAGCCAGCCCAACGGCCTGAGCACATGAGTGAGCAAGATT
TTGCCCTGATTCCTCAAACATTGGTCGTTAGAGAGGTGTGTTTGCGCTTATCCCTTAAGGGCTTTCGCGACCAGCACATTATTGTGGTGACGACGCTGCT
GGATGCTCAACGCTACAGCGCTGGGCAACTGACTCGCTTGTATGGCTGGCGTTGGCCAGTGGCGGAAGTCAATCTGCGCCATCTCAAAACCACCTTAAAA
ATGGAGATGCTCAGTGCCAAAACTCCGGATATGGTGCGCAAGGACATTTGGGTACATTTGTTGGGCTATAACCTACTCAGAAGTCTCATGGAACTTGCGG
CACCGCTAGCAGATAATGCTAGAACTCAACTGTCTGTGCAAGGAGCACGACAACACTTCAATCAGATGCTTGCTTTGTTGGCGACAGCCAACCGTGCGAC
CAGAAAGCGGTTGTTTACTCATCTACTTGAGACCATGGCAGCCGATCTATTACCCTCTCGACCGAATCGGCACGAACCGAGAGTCGTCAAACGCAGACCC
AAATCTTTCCCGCGAATGCGACAACCTCGCTCTGCTCTCAAAGCTAAGCTAGCTGTTTGATTGAGCTTAAGTCAGTGCCATTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1419 bp	472 aa	42	1460	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADKSLRNTVKCITTWLTAAGIQP
PSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSAANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIA
SWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQHRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVC
LRLSLKGFRDQHIIVVTTLLDAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHFNQMLALLATANRATRKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKAKLAV

Blast result :

Comments

ISAcma15 is 57% aa similar to ISArch10.

References

1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)