ISfinder

ISVsa14

Family ISAs1
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_011312	ND	Aliivibrio salmonicida	Aliivibrio salmonicida LFI1238 chromosome 1

DNA section

IS Length : 1276 bp

Ends

IR Length : 13/15

IRL : CAGGGTCCACGCATGTTATTTACGCAGTGATGCCTAATATTTCGGGATAT
IRR : CAGGGTTTACGCATGAGTTGCCAAAATGTTAAATTACAGCTAAGTGCCAA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GAATAAAGGCGCTGTTGACC		AACCCAAGGTTTACAAGGGA	0

DNA sequence

CAGGGTCCACGCATGTTATTTACGCAGTGATGCCTAATATTTCGGGATATAATTTTTTAAAATAGCTTGAATTCGGTGTTTAGAAATGATCTTATACTGT
TAACAAAAAAAATGAATTACGACGAGTTTAAAGAGCATTTTAACATATTAAGCGATACACGTCAGTCGAGAAAATCGACGTATAATTTCTTTGAAGTAAT
GTTCCAAGTAGTTACCGCAATGTTGTGTGGAATGAAGACCTGGGACGAGATAGATACCTTTGGTGAAGAGAATTTAACGTGGTTTAGAAAATTTAGCGAT
TACTCTCATGGAGTACCGAGCCACGATACCATTGCTCGAATAGTTAGCTTGGTTGACCCAGATGAATTTTCAATTTGTTTTACACGGTGGTGCAATGATA
TTCAGTATGAAAAATCATTAGATACGACTCATGTAGCGATTGATGGGAAATTATTAAGAGGAACATATGACTATAGTAAAAACAAATACTTAACTCATAT
GGTAAATGCTTATTCTGTTGATACAGGCTTGGTTTTAGGTCAATTAAAAACAGATTCTAAGAGTAATGAAATTACGACAATTCCTGAAATACTAAAGCTA
ATTAAAGTTAAAAACAGAACTATTAGTCTTGATGCAATGGGCTGTCAGAGAACAATTGCAGCAGATATTGTTAATAGAGAAGGTGATTACTTATTAGCAG
TGAAAGGTAACCAAGAACGCTTACATAATATGTTATTTAAATAAATTTTCTTTTGCTCAACTTGCTCATTTAGAGTGTGATAAATTTGAAACGGAAGAAG
TCGGTAAGCGCGGAAGCAAAATGGTTAGGTCTTATTATGTTGTGCCATTTACAGAAGTATTTGGAGACTTCTCTGTTGATTGGAAAGGTCTAGAAAGTTT
ATGTATAGCAGTGACTTATAATAAACATAACAAAAGTAACTCAGGCTCTTTAGGTATTAGATATTTCATATCATCTAAGAAATTATCAGCAGAAGAGTTT
GGTAAATTATGCCGAGGCCATTGGGGTGTAGAAAGCATGCATTGGTGGTTGGATAGCGTAATGAAAGAAGACGATAGCAAGATAATGTATCAGCATGCTG
CAGAGAATTTATCTAGGATCCGCCAGATGTGTATGAACTTCATAAAATTGGTGAATTTAAAAGGAACGTTAAAGAAACGGCAGTTGAAATGTGCACTTAA
TACAGACTTTAGAGAGATAGTATTATTTGGCACTTAGCTGTAATTTAACATTTTGGCAACTCATGCGTAAACCCTG

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1126 bp	374 aa	112	1237	+	Yes

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

Blast result :

Comments

ISVsa14 is 65% aa similar to ISEc26. There is a ponctual mutation at position 743, that generate a frameshift.

References

1] ISfinder annotation (2009)
2] Hjerde,E., Lorentzen,M.S., Holden,M.T.G., Seeger,K., Paulsen,S., Bason,N., Churcher,C., Harris,D., Norbertczak,H., Quail,M.A., Sanders,S., Thurston,S., Parkhill,J., Willassen,N.P. and Thomson,N.R. (2008) Direct submission GenBank.