ISAcma26

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017 plasmid pREB7
Acaryochloris marina MBIC11017 plasmid pREB6
Acaryochloris marina MBIC11017 plasmid pREB3
Acaryochloris marina MBIC11017 plasmid pREB4
Acaryochloris marina MBIC11017 plasmid pREB8
Acaryochloris marina MBIC11017 plasmid pREB5
Acaryochloris marina MBIC11017 plasmid pREB2
Acaryochloris marina MBIC11017
Acaryochloris marina MBIC11017 plasmid pREB1
DNA section
IS Length : 2669 bp

Ends


IR Length : 11/15

IRL : TATTAGACGACAATCGAGCTGACGGATTAATGACGATGTCACGTCGGCTA
IRR : TATTGGATGTCAACCCCATAGGCGATGTCGCGACAACCACAAGGCGGATC

Insertion site


Left flankDirect repeatRight flankDR Length
ACAGACATACCGAGGGCTTTCGACAG6
AAGCGATGCCAGCTTATGTCCATTTG6
TATTAAATCCTTCCACAAAACCACTG6
GATGTAGAGCATGAGGTTCGTCCCTG6
CGGCAATTCCTCAGATAGTTGAACCA6
GTCGATATCGCCCAATACTGCTACCA6
CGGACCAACCATTGCCACGGTCTAGA6
ATTGATGAATACCATCATCAGTCGAA6
TGTTTCATAAATCTTCTGTGTCACAG6
GTTTCTTGAGGCTAAATTAT0
AACGCTCCTCATTCAGAGGA0
GTTCAAAGCTGCCCAAATTT0

DNA sequence

TATTAGACGACAATCGAGCTGACGGATTAATGACGATGTCACGTCGGCTATAGCTATGAAGAAACCAGGCATCCCCTGAGAAGGATAAAACTAGATCTCA
ATTCCCTAGAAATGATGTGTGAGATCGCGTTTCTAGAATTCAATCTCAAGAAAAAAGACACTTTTTACATCCCTAATCAATAGAGGGCTGTACAAGTGCC
TAACAAAAGAATTGATTCCTATCATACTCGTGTTTATATGAACGGAAGAGACCTCGGCTTAAAACAAGCTGATGCAGCTTACATTGCCGAAATATCTACA
AGAACCGGACAACGAATTGAGGCCGGCACCCATCAACCTAATCGCGGCCGCCTTCAGGACCAACGCACCGTTCCCGACCCCTTAGCTGATGTGTGGGAAG
ACGAGCTAGAACCAATGCTGCGTCGAGACCCACGCCTCAAACCCATGACCCTGTATGAGTACCTGCAGGATAAGTATCCAGGCCAGTATCCCCAAGTCCT
GCGGACCCTACAACGTCGGGTGAGAACGTGGAAAGCCTTACATGGACCAAGCCCTGAAGTGATGTTTGAATTGCGTCATGAACCAGGGGTACAAGGGTTC
TCCGATTTTACAGAACTCAAAGGCATCACGATTACCATTGCCGGCAAACCCTTTGAGCACCTGATTTACCATTACCGTCTGGGATACAGCGGCTGGCGAT
ATGCCCAGATCATCGAAGGAGGCGAAAGCTTTGTCGCCCTCTCAGAAGGATTGCAAAATGCCTTTGCAGCCTGTGGAGGTGTTCCTACACAGCATCGTAC
TGATAGTTTGAGTGCAGCCTATCGCAACATGGGCGGCCGCCGGTCCAAAAACCTCACTCGTCTGTACGACGAACTGTGTGACCACTATCGGCTAGAACCC
ACTCGTAACAACAAAGGTGTAGCCCATGAGAATGGCTCCATTGAATCTCCCCATGGTCATCTGAAGAACCGAATTAAGCAGGCGATCTATCTGCGCGGCA
GTGCAGATTTTACGAGCGTTGCTGAGTATCAAGCCTTAATTGATGCACAGGTTGCCAAGTTGAATCAGCAGTGCCAAACCAAGTATGAGCAAGAGAAAGA
CCATCTACAACCACTGCCCAAATATCGAACCCCTGACTATGAAGTGCTCACGGCTAAAGTCAGCAAACGCAGCACCATCGATGTTCGCTGCATTCTATAC
ACCGTCCCTTCTCGACTGATTGGTCGTCAATTGGAACTGCATCTATACCATGACCGGATTGTCGGCTATCTGGAGCGACACCCGGTGGTGGAATTGCCGA
GGAAGCGCGTCAGTGGCAAAGGCAAACGTCGCGACCGTTGCATCAACTATCGCCATGTTATTGGTTCAATGCGATTGAAGCCTCGTGCTTTTATCTATTG
CACCTGGCAATCAGACCTACTTCCCAATCCTGAATACCGCCAAATCTGGGAACAGCTCAAAGCCCAATTTGACCTGGAGCAGGCTGCCAAGATCATCGTG
GAAGCCCTGTATATTGCTGCGGTTCAAGATAAAGAACAGGCCGTAGCAGTGTACTTACAGCAGCAGCTTCGCTCATCCAGCCTTACCCTCAATCGCCTGA
AAAAACAGTTTGAGCCGCCTCAGATGAAGCAGGTTCCTGAACTCAGCATTGAACAACATTCACTTGAACTTTATGACAAACTCCTCCCCTCCTGCTCAGT
CCCCGCTGAGCCCCTACCAGCACCTGAGCCTCTATTTAAAAAAGCTCAGGCTCTCCCACATGTTGACCCATTGGGAATCTATCGAATCCCAAGCCATGCA
GGAAAACTGGTCTTATGCGGAATTCTTACTAGCCTTGTGCGAAACGGAGGCCCAACGAAGAGAACAAGCTCGTCTAAAACGTGCCCTCACCGAAGCCAGG
CTCCCAAACGCAAAAAGTTTTACCAACTTTGACTTTAGCCATTGTCCCCAGCTCAATCCAGCTCCCTTGATGCAATTAGCCGCAGATCCGGGTTGGTTGG
AGCGCGCCGAGAATTGCCTTATTCTGGGGCCCTCGGGTGTTGGAAAAACACATCTGGCCACTGGGGTGTCCAAAAAGATGCTGGAATTCGGTAAGCGGGT
GAAGTTCTTTGCAGCCAACGCATTGGTCCAGCAACTGCAACAGGCGAAGCTCCAACTGCAGCTGCATCCAATGCTCAAAAAACTGGACCGCTATGATCTG
TTGATCTTGGATGACTTGGGCTATTGCAAAAAGTCGGAAGCGGAAACGTCCGTTCTGTTTGAATTAATTGCGCATCGCTATGAACGAAAAAGTTTGTTGA
TTACAGCGAATCAACCCTTCAGCCAATGGGATGACATTTTCACTGATTCGATGATGGCGGTGGCTGCCATTGATCGCTTAATTCATCATGGCTTGATTAT
CGAAATTCAAGCCGATAGTTATCGGCGCAAATCAGCGACTCAGAGAACGGCTCAAACTCAGTCTCAACCTCAAAAATCCCAGTCCAAATAATCGAGCGAT
CACTGGTTGTCATTTCGATCACGGGTTGTCGTTTTGTTCTGAAGGTATCGTATGAGGTCTGAAAAAGAGCTAACGCAAAGGGAGTGTACGCCAAATAATA
CGGTTCAAATTTGGGGGTTGATCCGCCTTGTGGTTGTCGCGACATCGCCTATGGGGTTGACATCCAATA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1737 bp578 aa1961932+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VPNKRIDSYHTRVYMNGRDLGLKQADAAYIAEISTRTGQRIEAGTHQPNRGRLQDQRTVPDPLADVWEDELEPMLRRDPRLKPMTLYEYLQDKYPGQYPQ
VLRTLQRRVRTWKALHGPSPEVMFELRHEPGVQGFSDFTELKGITITIAGKPFEHLIYHYRLGYSGWRYAQIIEGGESFVALSEGLQNAFAACGGVPTQH
RTDSLSAAYRNMGGRRSKNLTRLYDELCDHYRLEPTRNNKGVAHENGSIESPHGHLKNRIKQAIYLRGSADFTSVAEYQALIDAQVAKLNQQCQTKYEQE
KDHLQPLPKYRTPDYEVLTAKVSKRSTIDVRCILYTVPSRLIGRQLELHLYHDRIVGYLERHPVVELPRKRVSGKGKRRDRCINYRHVIGSMRLKPRAFI
YCTWQSDLLPNPEYRQIWEQLKAQFDLEQAAKIIVEALYIAAVQDKEQAVAVYLQQQLRSSSLTLNRLKKQFEPPQMKQVPELSIEQHSLELYDKLLPSC
SVPAEPLPAPEPLFKKAQALPHVDPLGIYRIPSHAGKLVLCGILTSLVRNGGPTKRTSSSKTCPHRSQAPKRKKFYQL

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
819 bp272 aa16732491+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MTNSSPPAQSPLSPYQHLSLYLKKLRLSHMLTHWESIESQAMQENWSYAEFLLALCETEAQRREQARLKRALTEARLPNAKSFTNFDFSHCPQLNPAPLM
QLAADPGWLERAENCLILGPSGVGKTHLATGVSKKMLEFGKRVKFFAANALVQQLQQAKLQLQLHPMLKKLDRYDLLILDDLGYCKKSEAETSVLFELIA
HRYERKSLLITANQPFSQWDDIFTDSMMAVAAIDRLIHHGLIIEIQADSYRRKSATQRTAQTQSQPQKSQSK

 

Blast result :
Comments
ISAcma26 is 64% aa similar to ISEc10.
This IS has atypic IRs: it begins with 'TA' instead of 'TG'.
References
1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)