ISAcr4

  • Family IS1380
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009469 ND Acidiphilium cryptum
Acidiphilium cryptum JF-5
Acidiphilium cryptum JF-5 plasmid pACRY03
DNA section
IS Length : 1618 bp

Ends


IR Length : 13/16

IRL : CCGGAATTATGCATGAAGGTTGGCGAGCATAGCGTAGTCCATTGATTCGA
IRR : CCGGGATTATTCACGAGCGCATTTCCTCTGATTTTTCTCAGGCGGTCTGT

Insertion site


Left flankDirect repeatRight flankDR Length
AAAAGCGGTACTATCATATCGAGA4
GCCACCCATGCTAGGAAGCTCAAG4

DNA sequence

CCGGAATTATGCATGAAGGTTGGCGAGCATAGCGTAGTCCATTGATTCGATGTAAGAATTTGTTGTCGAGACAAATCTTCACCACGACGGAACGCGATGC
TCGCCTCGGTTGCAAATACCCCAATTCTCCCTGGTCTGTCACCTGTCGCCGGCAAATCGATCGAGGCCCGCTTCGACGGCGAGATGCTGTCCTCTGACGG
CGGTCTGCTGGCATTGCGCGAGGTCGAGAAGCGGCTGGGGATTGCCGCGCAGTTGGCAGCCTGCATCGATGATCCGCGCACGCCGGAGCGCGTCCAGCAT
GGGCTCGACGAGATCATTCGGTTTCGCATGTTGATGATCGCGGCCGGTTACGAGGATGGCAACGACGCCGACCGGTTGCGCAACGACCCGATGTTCAAAC
TCGCGATGGAGCGTCTGCCCGAGGCGGGCGATCTGTGCTCGCAAGCGACGATTTCACGCACCGAGAATTTGCCCGGTCCTCGCGCGCTGCTGCGCATGGG
CCTGGCGATGGTCGAGCATTATTGTGCGAGTTTCCGCACCATACCCAACCGGGTCGTGCTCGATATCGACGATACCTTCGATGCCGCTCACGGCGCGCAG
CAACTCTGCTTGTTCAACGCACACCACGATGAGTACGGTTTCCAGCCGATTGTGGTATTCGACGGCGACGGCCGGATGCTCGCCGCGGTGCTGCGTCCGG
CGTGCCGGCCAAAAGGTAGCCAGATCGTCAAATGGTTGCGCCGGCTGATCGACGCCATCCGCAGCCACTGGCCCCGCACCGCGATCATGCTGCGCGGTGA
TTCGCACTACTGCACGCCGGAGGTGCTGCGGTTCTGTCGGGCGCGTCGCCTCGATTACATCTTCGGCGTCGCACCGACCACGACGCTGCGCAAGCACGTC
ATCGCGCTGGAGGCCAGCACCACGGCGCGCGCGCAACAAGCTCCGGGAGAGAAAATCCGGCGGTTCAAAGAGTTCAATGATGGCGCAGCAAGCTGGGACC
GCGTCGAGCGGATCATCGCCCGCGTCGAGGCCGGGCCGATGGGGGTCGATACCCGCTTCATCGTTACCAGCCTGAAGGCAGGTTCCCCGCGCACCCTGTA
TCAAGAGATCTACTGCGCGCGTGGCCAGGCCGAGAACCATATCAAGGCGTGGAAGACCCATCTTGCCGCCGACCGGACCTCGTGCTCGCGGGCCAGCGCC
AACCAGATGCGCCTGTTTCTGCATATCGGCGCCTACTGGCTGATGTGGAGTTTGCGCTCCCTGATGCCGCGCCGGTCGCGCTGGCGTGGTATCCAGTTCG
ATACCCTGCGCCTGCGCCTGATCAAACTCGCCGTCCGCCTCGAAACCCTGAAACGCTCGATCCGTCTGCATCTGCCGCGCTCAATGCCAGACCAGGCGAT
CCTCGGCTATGCCCTGGCCAGACTGCCCAGGCTGGTCATCTGATCACAGGGGCGTTTGCCCCGTCCGGTCCCGATCTCTGCCACATCCAACGCCAACACA
TCATAGAGCCCACCAAAACCAGCCAAGTTCGGCCGGTAAGCCATCACGCCCGCACGACGTCAGGTTGAACAGACCGCCTGAGAAAAATCAGAGGAAATGC
GCTCGTGAATAATCCCGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1347 bp448 aa971443+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLASVANTPILPGLSPVAGKSIEARFDGEMLSSDGGLLALREVEKRLGIAAQLAACIDDPRTPERVQHGLDEIIRFRMLMIAAGYEDGNDADRLRNDPMF
KLAMERLPEAGDLCSQATISRTENLPGPRALLRMGLAMVEHYCASFRTIPNRVVLDIDDTFDAAHGAQQLCLFNAHHDEYGFQPIVVFDGDGRMLAAVLR
PACRPKGSQIVKWLRRLIDAIRSHWPRTAIMLRGDSHYCTPEVLRFCRARRLDYIFGVAPTTTLRKHVIALEASTTARAQQAPGEKIRRFKEFNDGAASW
DRVERIIARVEAGPMGVDTRFIVTSLKAGSPRTLYQEIYCARGQAENHIKAWKTHLAADRTSCSRASANQMRLFLHIGAYWLMWSLRSLMPRRSRWRGIQ
FDTLRLRLIKLAVRLETLKRSIRLHLPRSMPDQAILGYALARLPRLVI

 

Blast result :
Comments
ISAcr4 is 71% aa similar to ISRssp8.
References
1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Magnuson,T. and Richardson,P. (2006) Direct submission GenBank.