ISfinder

ISKol1

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012785	ND	Kosmotoga olearia	Kosmotoga olearia TBF 19.5.1

DNA section

IS Length : 2383 bp

Ends

IR Length : 40/58

IRL : TGTATGTGGTCAAATAAAATTGACCCCCCAGGGCCATAGAAAATTGACCC
IRR : TGTATAAAGTCAATAGGTTTTTGACCCGGGAGGGCCATAAGGAAATGACC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGAGCTCTAA	GCTTAT	CCAATAATTG	6
CTCCACTCTG	CAAAGT	GGAGGGTTTG	6
CCTGAGATGG		ATTGGAAAAG	0
AAAAAAACGT	AACTGG	ACAAAAAGAA	6

DNA sequence

TGTATGTGGTCAAATAAAATTGACCCCCCAGGGCCATAGAAAATTGACCCGTTACCCCGTGGAAAAAAAGACCTTCTTGAGCGAGATAATAGAAACAATC
TGCTCAAGGAGGTATACGAGGGGTGAAAAACGTGTACGAGGTCAAAGAGATTCAACGGCTCTATCAGGAATATGGTTCTATTCGTGAGGTAACAAGACTC
ACCGGGATATCCCGGAACACTGTTAGGAAGTATTTACGCAGAATCGAAGCAGTGAAATTAGGAAGCGCAAAGGAAGTGATTGTTAGGAAGCCCGTTCCTT
TTAAGCGTCGGACGATTGAGCCAAGGACAGTAGCGAGGATTGAAGAACTCCTGGATTCCAACAAGGACAAACCCAAGAATTTGCAATTCACCGCGAAGAA
AATCTGGCAAATGGTGGTTAAGGAAGGACACAAGATAAGTTATACCTCGGTGAAAAGGATTGTTCGCAAATGGAAGGAAGAACACGGTCAAAGGGATGTC
TACATCATTCAGAAGCCGGATGGTAAACGAGCTGAATTTGATTGGGGAGAGGTGGAACTGGTCATTGCCGGTGAAAAAGGGAAATACCCTGCTGCATTCA
TGGTACTGAATCGTTCGCTGTATCGTTTTTCCAGGGTCTTTGAAAGAGAAACTGCCCAGGAGGTAATCCAGGCACACATAGACTTTTTCGAGGAGATTGG
TGGTGTACCACAGGAGATATTCTACGACAACATGAAGGTGGTGAAAGACAGGAAGAACCTGAACAAGAACTTCGTCAAATTTGCCACCTTCTTTGGATTC
ACTCCCAGGTTGTGTAATCCTTATTCACCCCAGGAAAAGGGGACAGATGAGGAAAGTGTTGGTTTCGTTAGAAATTGGGTATTCAGTGAAAGGAATGAAT
TCGATTCCCTGGAAGAAGCGAATGAATATTTAAAAGAAAAACTGGCAGAACTAAATGCTTCTCCTGTATATGGAAGAGAGTTAGTTCCGGTAAAAGCTCT
CAATGAAGAAAAACTAAATGCATTACCTACCGCCAGCTACAACAACTATCGTCTTGAAGAAAGGATCATATCCAGATATTCGTTAATAATGCTGGACGGT
AATTACTATTCTGTTCCTGAGGATTGCCCCGGGAAGAGAGTGCAGCTGAAGATATATCCAGACCGTATAGAAATATTGAAAGAAGGCAAAGTCGTTGCGG
TTCACAAACGCTTAAGAGGTAAAGGTAAATACGCCATACAGATAGCTCACTACCTGAACACCCTAAGAAAAAAGCCTGGAGCCCTTGCAGGATCAAGAGC
CTTCCAGGCATTAAACGACACACTAAAAGTCGTGTTCGGCAAATATTATACCACTAAGCCAAGGGAATTCGTACAATTACTGGAATTATTGAACGATTAT
GACGAAAAAACCTTCTGCGAAAAAATAGAAGAGCTCTTTGAAAACGGGATTATCCCAACAGCAGACATAATGAGAAACCTATTACAACAAAAGCCCATAC
AATATGAAACCTTTGACTATCCTTTGAAGATACAGATAGACCACGGTGATCCTTCTGAGTTCGACAGATTGGTAGGTGTTCACCGTGGATGAAAAACTAA
TGAGATTGTGCAAAAGGCTGAAAACACCGGGAATATTCAACAGTTACGAGAGAATCACTAGACAAGCTCAACAAGAAGGCATGAGTTACATTGAATTCCT
TCTGGAGATACTGGAATCGGAAGTACAATCCAGAGAAAGCAGAAACATAGTGAAAAAAATAAAACGGGCTGGATTTCCAACACCCAAAAGATTTGAAGAA
CTCAACGAAGCAGCCCTTCCAGAAGACGCCAGGAAACATCTTAAAGAACTGAAATCGTTGAAATTTTTAAGCGAGAATAGAAACCTAATACTGCTGGGTA
ACTCCGGTACAGGAAAAACACATCTGGCAACGGCAATAGGAATAAAAGCCTGTGAAGAAGGATATAACGTTCTATTCAAAACAGCGGCAGGCCTGATTAA
CGAACTGAAAGAAGCAAAAGACGAAAAACAACTTGTGAGATACGCAAAACAATTCAAAAAGCTCGATCTGGTGATAATAGACGAATTGGGATACATTTCC
TTTGACATTGAAGGTGCAGAGCTGCTCTTCCAATACCTGGCAATGAGATACGAGACAAAGAGCACGTTAATTACAACGAATCTCGTGTTCTCGGAATGGA
TAAAGATCTTCCATGATAAAGCCCTAACCATGGCATTATTAGATAGAATCACACACAATGCCGTGATTCTCAATATGTCTGGAAGAAGTTACCGAAGAAG
GGATATTTTAGAAGATACCTGAGGTGGGTAGCGGGTCATTTCCTTATGGCCCTCCCGGGTCAAAAACCTATTGACTTTATACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1470 bp	489 aa	123	1592	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VKNVYEVKEIQRLYQEYGSIREVTRLTGISRNTVRKYLRRIEAVKLGSAKEVIVRKPVPFKRRTIEPRTVARIEELLDSNKDKPKNLQFTAKKIWQMVVK
EGHKISYTSVKRIVRKWKEEHGQRDVYIIQKPDGKRAEFDWGEVELVIAGEKGKYPAAFMVLNRSLYRFSRVFERETAQEVIQAHIDFFEEIGGVPQEIF
YDNMKVVKDRKNLNKNFVKFATFFGFTPRLCNPYSPQEKGTDEESVGFVRNWVFSERNEFDSLEEANEYLKEKLAELNASPVYGRELVPVKALNEEKLNA
LPTASYNNYRLEERIISRYSLIMLDGNYYSVPEDCPGKRVQLKIYPDRIEILKEGKVVAVHKRLRGKGKYAIQIAHYLNTLRKKPGALAGSRAFQALNDT
LKVVFGKYYTTKPREFVQLLELLNDYDEKTFCEKIEELFENGIIPTADIMRNLLQQKPIQYETFDYPLKIQIDHGDPSEFDRLVGVHRG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
723 bp	240 aa	1600	2322	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MRLCKRLKTPGIFNSYERITRQAQQEGMSYIEFLLEILESEVQSRESRNIVKKIKRAGFPTPKRFEELNEAALPEDARKHLKELKSLKFLSENRNLILLG
NSGTGKTHLATAIGIKACEEGYNVLFKTAAGLINELKEAKDEKQLVRYAKQFKKLDLVIIDELGYISFDIEGAELLFQYLAMRYETKSTLITTNLVFSEW
IKIFHDKALTMALLDRITHNAVILNMSGRSYRRRDILEDT

Blast result :

Comments

ISKol1 is 51% (ORFA, the transposase) aa similar to ISSau9 and 65% (istB, the helper of transposition) to ISCth9.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G. and Noll,K. (2009) Direct submission GenBank.