ISfinder

ISKol2

Family IS256
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012785	ND	Kosmotoga olearia	Kosmotoga olearia TBF 19.5.1

DNA section

IS Length : 1332 bp

Ends

IR Length : 23/29

IRL : GGGTCTGTTAGTAATTTTGTGTAAACTCCCTCCGAAATATGAGAAAATAA
IRR : GGGCATGTCACATATTTTGTGTAAACTCCAAACTCCGGAAAAGATCAGAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCTGAAGAAG	GAAAACTC	TGTAATGCAC	8
AGGACGGGGG	GATTCCCCC	CCTCCTACTC	9
TTCAGAACAA	CCACGTAG	CCACGTAGTG	8
GTGTAATTAG	CTGTAAAC	CTGTAAACAG	8

DNA sequence

GGGTCTGTTAGTAATTTTGTGTAAACTCCCTCCGAAATATGAGAAAATAACAAAAAGCTAAGGAGGGAGAACAGTGGGAAACTTCGACAGAGAAGCAATG
AAGAGATTGGTACGAGAAAGGAAATTAAAAACGACAAAAGACGCGGAAAATCTAGTAAAGGAATTATTTGGAGACATCATTAAAGAGATGTTGGAAGCGG
AACTTGAAGAAGAACTTGGTTATTCTAAGCATGACTACAGAAACAAAGAAACGGATAACTCACGAAACGGTTATTCGAAAAAGACAGTGAACAGCTCTTA
CGGTAAAATAGAACTTTCGATTCCCAGGGATCGCAAAGGTGAATTCGAACCAATAGTGGTTAAAAAGCACCAGCGAAGCATAGCTTCCATAGAGGACCAG
ATATTGTCTATGTATGCCCGGGGAATGACATACAGGGACATACAGGCACATCTTAAGGACATATACGGTTCTGAACTGTCAATTGAAAGTATAAGCAGGA
TAACAGACAAGATAATCCCTATTGTTTCCGAATGGAGAAACAGACCCCTTGAAGAGGTATATACCATAATCTACATGGACGCTATCTTTTTCAAAGTTTC
TGAGAACAACCAGATAAGAAACAAGGCTTTGTATATAGCCTTTGGGGTAAATCTGGAAGGAATGAAAGATGTCCTAGGAATGTGGATAGCTCAGAGTGAA
GGTTCAAAATACTGGCTTGGTGTGCTAAATGAACTGAAAAACCGAGGAGTTAAAGGTGTAATGTTCTTTTCAATAGATGGATTAAAGGGAATGGAAGAGG
CAATAGAAGCGGTATATCCCCAGGCTAAAATCCAGAGATGCGTAGTACACCAGATAAGATATTCCATGAAATTTGTATCCTGGAAAGACAAAAAGAAATT
CGCTGCTGATCTGAAATCGGTATACACAGCGGATACAAAAGAAGCAGCATGGAATGCTCTTATGGCCTTTGAAGAGAAATGGGGAGAAAAGTATCCATTC
AGCATTAAAAGCTGGAAAGATAACTGGGAAGCCCTCACGACCTATTTCGAATATCCCAAAGAAATTAGAAAATTGATTTACACGACAAATCCCATAGAAT
CCCTGAACAGCCAACTCAGGAAGGTAACAAAGAACAGAGGTGTTTTCCCCAATGATATGGCTCTCATGAAACTTGCATATCTCGCCATTAACAACATAAC
CAGAAAATGGACCGTGAGAATAAACGAATGGGATAAAATCCTTGCCACTTTGATGATAGAATTTGATTGGGTGAAATCCTATGTCTGATCTTTTCCGGAG
TTTGGAGTTTACACAAAATATGTGACATGCCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1215 bp	404 aa	74	1288	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VGNFDREAMKRLVRERKLKTTKDAENLVKELFGDIIKEMLEAELEEELGYSKHDYRNKETDNSRNGYSKKTVNSSYGKIELSIPRDRKGEFEPIVVKKHQ
RSIASIEDQILSMYARGMTYRDIQAHLKDIYGSELSIESISRITDKIIPIVSEWRNRPLEEVYTIIYMDAIFFKVSENNQIRNKALYIAFGVNLEGMKDV
LGMWIAQSEGSKYWLGVLNELKNRGVKGVMFFSIDGLKGMEEAIEAVYPQAKIQRCVVHQIRYSMKFVSWKDKKKFAADLKSVYTADTKEAAWNALMAFE
EKWGEKYPFSIKSWKDNWEALTTYFEYPKEIRKLIYTTNPIESLNSQLRKVTKNRGVFPNDMALMKLAYLAINNITRKWTVRINEWDKILATLMIEFDWV
KSYV

Blast result :

Comments

ISKol2 is 73% aa similar to ISDet4.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G. and Noll,K. (2009) Direct submission GenBank.