ISfinder

ISKol5

Family IS256
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012785	ND	Kosmotoga olearia	Kosmotoga olearia TBF 19.5.1

DNA section

IS Length : 1268 bp

Ends

IR Length : 23/30

IRL : GGCAGTATAAAATAAATGCTGTAAAAATAAAAAAAGCCCTGTATCCTAGA
IRR : GGCTATGTCAAGAAAGTGCTGTAAAAATAACCCTGTATCCTAAAACAGGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCCCTATATA	AACCACAT	GGGTACCAAC	8
GAAGTTTCAA		AGGAAAGTGC	0
ATTTTGCGGG	TCTTTTTT	ACTACGAGTA	8

DNA sequence

GGCAGTATAAAATAAATGCTGTAAAAATAAAAAAAGCCCTGTATCCTAGAATAGGAAGAACACATAAACCATACAAAGGAGGGATACAGGGCCCATGGAA
ATCAAAGATATTATACTCCAATTTCTCACAGAAAATGAAGAAGGAATGAGAAATCTTCTAACCTGGTTTTTGAATACGGTTATGGAATATGAAGCCCGGA
TACAGGCTGGAGCAGACTACTACGAGAGAACAAAAAACAGAAGAGCACACCGCAACGGTTATAGAAAAAGAAGTTTGAACACCAGATTAGGAAAATTGGA
GTTGAAGAAACCCCAATTCAGAGAATTCCCCTTCGAAACCAGGGTCTTTGATAAATACTCAAGAGTAGAGAAAGCATTAAAGAACGCTATTGTCGAATCA
TATCTTCAGGGTGTTTCTACTCGCAGGATAAAGGAAATAATATCCACCTTAGGAGCAGAAGAACTTTCACCTGGAACAGTTTCTAATATAGCCAGGGAAC
TGGACGAAAAGGTGTATGAGTTTCTTAACAGGGAAATAGAGGAGGAGATACCGTATCTTTTCGTGGACGCCAGCTATTTCAAAGTCAGGGACGGGGTTAG
ATACGTCAATAAAGCTCTTTATGTGGTAGCCGGTGTGAGAAAAGATGGACATAGAGAGATACTTGGTGCAAAGATCGCCGATGTTGAAGATGAACTCTTT
TGGGAAGACTTCTTCCAAGAATTGAAAAACCGTGGATTGAGAGGAGTAGAACTTGTCATCTCAGATGGTCACAAGGGGATAATCTCAGCGGTGAAAAAGG
CCTTTATTGGAGCGAGCTGGCAAATGTGCCATGTACATTTTGTGAGGGATGTGCTGAAAAAAGTACCTCGTAAACGCTGGAAAGAGATAGCAGAACGGTT
GAACGAGAGCCTGGAAAGTGCGAAAAAATTACAAGCCTTTATTGAGGAACTTGAAGAAGAAGGCTTCAAAAAAGCGGCGGAAACCTGTGAAAGATTCATA
TTCGGCTTGTTTAATTACCAGAAATATCCACGGGATCATTGGAAAAGGATAAAAACGACGAATATGCTGGAACGGATAAACAAAGAACTAAAAAGGCGTA
CTAGAGTGGTAGGAGCTTTTCCAAATGATGCGGCACTTTTGAGGTTGGCTGTCGCAATCTTACTTGACATTAACGAAGAATGGCTGCTTGGAAGGAGATA
TCTAAACATGGGATCTTAGCCTGTTTTAGGATACAGGGTTATTTTTACAGCACTTTCTTGACATAGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1125 bp	374 aa	95	1219	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEIKDIILQFLTENEEGMRNLLTWFLNTVMEYEARIQAGADYYERTKNRRAHRNGYRKRSLNTRLGKLELKKPQFREFPFETRVFDKYSRVEKALKNAIV
ESYLQGVSTRRIKEIISTLGAEELSPGTVSNIARELDEKVYEFLNREIEEEIPYLFVDASYFKVRDGVRYVNKALYVVAGVRKDGHREILGAKIADVEDE
LFWEDFFQELKNRGLRGVELVISDGHKGIISAVKKAFIGASWQMCHVHFVRDVLKKVPRKRWKEIAERLNESLESAKKLQAFIEELEEEGFKKAAETCER
FIFGLFNYQKYPRDHWKRIKTTNMLERINKELKRRTRVVGAFPNDAALLRLAVAILLDINEEWLLGRRYLNMGS

Blast result :

Comments

ISKol5 is 80% aa similar to ISMma16.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G. and Noll,K. (2009) Direct submission GenBank.