ISfinder

ISKol6

Family IS110
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012785	ND	Kosmotoga olearia	Kosmotoga olearia TBF 19.5.1

DNA section

IS Length : 1516 bp

Ends

Left end : CCGATGTAGGAGTAAAATAAAGGAGGCAGTCAGGAGGAAGAGAGGAACAGAGAACCGATCCGCCATAAGAGCGACTGCTTCTCACACTCCCTCTTCCATC
Right end : AGAAAGAAAAGTTATCCCTGCCTGTCTCTGTTGCTCAAACCAACTGCCATTTGGGAACAGTTTCAGGTAGTCTGTCTCCTTGACTTGACATAGTAGGTGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AACTCGAGAA		GAGCCGAGAA	0
AGTCCGAGAA		TGTTGGAGAT	0
ATCCGAGAGT		GAAAAAACAT	0
AGTCCGAGAA		GAGGGCCGCT	0

DNA sequence

CCGATGTAGGAGTAAAATAAAGGAGGCAGTCAGGAGGAAGAGAGGAACAGAGAACCGATCCGCCATAAGAGCGACTGCTTCTCACACTCCCTCTTCCATC
CTGGGAGTACTAGAGGATCAATAGTAGGATATCCGGGGAAACCCAGGAACGCTACATCGATTAACCTGTACTCATTGACTGCACAATGTTTTTTGGAGGA
ATCAGTATGGATATTGTGGGAATTGACTGGTCGTGGAATTTTCACACCTGCTATTCTATCGAACAGGATAAGGTTTTTAAGATCAAGGATGGTATAGCGG
GGTATGAGAAGCTGCTGAAAACAGTATCAAAAGACGCTGTTTTCGTCATTGAGGAGAGTTTCAATCGTTTGGGAGATTTCTTGCTGTCCAGAGGGAAAGA
GGTTTATCTCCTGCCACCGAAAAGGTCAAAGGAAGCCAGAGGCTATCATTCGAACGGCGTGAAGACAGATTCAACAGATGCCAGGTGTATCGCCCTTACC
TACAAAGAACACCCGGAATATTGTATTAAGGCAACACATGACGAACTCGGCATGCTTTTCAGGGAATTGTTGAGGCATTACAGGTACTATACCGACATGT
CCTCAAGACTTAAGAACAAACTTCAAAACGAACTTCATAATTATTTCCCTGAATATGCTAGGGTGATGGGTAATTCTTGGACAGATTGTGACGGGAGGAT
ATTCCTGCTCACCATATGCCCGGATGTAACCGAACTCAGGAAGACATCTGACAAGGAGATAAATAGACATTTCAAAGCGAATCATCTACGCTACACATCC
TCATTGAAAAGAAAGGTCAAAGAACTCAGGGAAAAAGCCATTGACTGGGGCATGTCAAAATACACCAGGGACATGATCGCGAATGTTGCCACACAACTTC
TGGAAGCAAAAAGAGAGAAAAAGAAAATAACAAAAGAGATGGAGAAAGAACTACGTAAATCGAAATACAACATAATACTGACGCTCCCGGGTGTTGGAGT
AGCTACAGGAATGGCATTAGTAACAGCATTTCTTACACACGAATTCAAAAATTACAGGGATTTCCAGAAGTATTGTGGAACTATGCCAATAGTATCCCAG
AGTGGTAATTTTCGCATGTGCAGGATGAGGAAAAACTGTGACAAGAAACTCAGAGGCATTCTCCATATGATGGCGATAAACGCGAAGAAATGCGGAGCAT
GGATGAAAGGCTACTATGACAAGAAGACAAATAAAGAGGGCAAGAAACCATCTCTTGCCCTCAGAGCATTGGCGAACATCCTCGTCAAGATAGCCTTCGC
CATGCTAAGAAACCTAAAAGCCTACGATGAGGAACTCTTCCTTTCCTCACGTGGGAACAAGAGTCCTCGGTTAAATTATACAACAAGAAATAGCATACCG
GGGACCAAAAAAATGAAGAAAGAAAAGTTATCCCTGCCTGTCTCTGTTGCTCAAACCAACTGCCATTTGGGAACAGTTTCAGGTAGTCTGTCTCCTTGAC
TTGACATAGTAGGTGA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1293 bp	430 aa	207	1499	+	No

ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MDIVGIDWSWNFHTCYSIEQDKVFKIKDGIAGYEKLLKTVSKDAVFVIEESFNRLGDFLLSRGKEVYLLPPKRSKEARGYHSNGVKTDSTDARCIALTYK
EHPEYCIKATHDELGMLFRELLRHYRYYTDMSSRLKNKLQNELHNYFPEYARVMGNSWTDCDGRIFLLTICPDVTELRKTSDKEINRHFKANHLRYTSSL
KRKVKELREKAIDWGMSKYTRDMIANVATQLLEAKREKKKITKEMEKELRKSKYNIILTLPGVGVATGMALVTAFLTHEFKNYRDFQKYCGTMPIVSQSG
NFRMCRMRKNCDKKLRGILHMMAINAKKCGAWMKGYYDKKTNKEGKKPSLALRALANILVKIAFAMLRNLKAYDEELFLSSRGNKSPRLNYTTRNSIPGT
KKMKKEKLSLPVSVAQTNCHLGTVSGSLSP

Blast result :

Comments

ISKol6 is 44% aa similar to ISCth14.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G. and Noll,K. (2009) Direct submission GenBank.