ISfinder

ISKol8

Family IS701
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012785	ND	Kosmotoga olearia	Kosmotoga olearia TBF 19.5.1

DNA section

IS Length : 1212 bp

Ends

IR Length : 18

IRL : CCCAAACTATGCACTTAGAAGAAAGAGGAAATACAGGTCATTGAAAAAGG
IRR : CCCAAACTATGCACTTAGCTAGATAAACCCAGAGAGAAAACAAAAGAACG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATGATATACT	AATTT	AGTATACTTA	5
TATACATAGT	ATCAT	GGAATAGCGT	5

DNA sequence

CCCAAACTATGCACTTAGAAGAAAGAGGAAATACAGGTCATTGAAAAAGGCATGGGAACCTAGTAAATTAGTTGTGAAGCCAAACACAACATATGGAGGT
TTCCCATGCCCACAACTAATATACCACAACTTCTAAATTCTATGTGTGAAGAGATTTCTTTTCCCAGGTCTGAGCACAAAACACTGCTGCTCGATTACAC
CAGAGGCCTTGCCATATCTCATTTCAAAAGCCTCAGTTCAATCGGTAGGAAAATGGGGCATTCTCAGTCATCTTTCTCACGAATGCTTTCAAACACCATG
GTTACCACTGAAGAGCTCACAGGCAGCAGATTGAATTTCATCCAAAACTTTCTTTCTAACAATGGTCTTGAACCCAAATACATCATATTGGATGAGACCG
TTATTGAGAGGTATGGCTCAAAGATAGAGAACCTTGGCAAGTATTACTCTTCCACAGCAGAGAAGGTAATAAACAGTATCTCGCTTATCTCATCTGTACT
ATATGTAAAGAACGGACTGTTTTTTCCCCTGGTTACAAGAGAAAGAACAAAGGGTGATTTCACAGGGCAACTCATCTCCATTCTTGAGTACTCGAAGCTT
GAAAACAACATTGTCCTTGTAGATGGAGCCATTATGTGCGGCAGGATATTTGCAAAGGTTGTCAATTCTGCTTCGACTATAATCGGCAGGCTGAGAACCA
ATCTCAAGGTTGTGATTGACGGAAAAAGCTTCTTGCTTTCAAAGCTCAAGAAAAAAACTAAGGGAGTCACATCATTTATTGCGAGAGTTCCGAGCTACCA
AGCAAAGGTAAAGATAGTCATTGACAACAGTGCTGACGAAACGAGAATCATTCTTTGCAGCGACACGAACATGACAGAGTATGAGATACTCAATCATTAC
AGCAAAAGAGAGACTGTGGAAGAATACTTCAAGCAGGCAAAAGGTCTATTGGGGCTGAAAACACAGGTTTACTCACAGCGTTCCACTGAAAAACACACGG
AGCTTGTAGCTCTCTACTTCACAGCGATGATGATAGCGAGATACTATTTGAATGTCAAAGACCGGATAGGATTCAGAGAATTCATTGAACGGCAGATAGA
ACAGTACCATCTGGAAAAAGCTTTATTTTCCCTATTTGCTTTATTTGTTCGTGATCCCGAATCGTTCTTTTGTTTTCTCTCTGGGTTTATCTAGCTAAGT
GCATAGTTTGGG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1089 bp	362 aa	106	1194	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPTTNIPQLLNSMCEEISFPRSEHKTLLLDYTRGLAISHFKSLSSIGRKMGHSQSSFSRMLSNTMVTTEELTGSRLNFIQNFLSNNGLEPKYIILDETVI
ERYGSKIENLGKYYSSTAEKVINSISLISSVLYVKNGLFFPLVTRERTKGDFTGQLISILEYSKLENNIVLVDGAIMCGRIFAKVVNSASTIIGRLRTNL
KVVIDGKSFLLSKLKKKTKGVTSFIARVPSYQAKVKIVIDNSADETRIILCSDTNMTEYEILNHYSKRETVEEYFKQAKGLLGLKTQVYSQRSTEKHTEL
VALYFTAMMIARYYLNVKDRIGFREFIERQIEQYHLEKALFSLFALFVRDPESFFCFLSGFI

Blast result :

Comments

ISKol8 is 43% aa similar to ISPlag1.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G. and Noll,K. (2009) Direct submission GenBank.