ISfinder

ISKol10

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012785	ND	Kosmotoga olearia	Kosmotoga olearia TBF 19.5.1

DNA section

IS Length : 2060 bp

Ends

IR Length : 34/49

IRL : TGTCGACATCGATTATAAAATAGTAAAAACGTCGGTCAAAAATGAGCAAA
IRR : TGTCAACATCAACCAAAAATGCAAAAAAACATCGATCAAAATTGCGTAAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGAGAAAGAA	GCTGG	GATATGTCAG	5
TGGTTCGAGG	ATGAG	TTTTAATGCT	5
GTGAAACAGA	TATGG	AAACCGCAAG	5

DNA sequence

TGTCGACATCGATTATAAAATAGTAAAAACGTCGGTCAAAAATGAGCAAAAACATCGGTAAGAATTGAGTAGTAACATCTGAATATCTCAGATAGAAAAG
GAGGTATTCAGATGTTAACGGAAAAGCAGGTCTTTGAAATCAGAATATTGTACAGAGAGGGGTTAAGCAAGCTAGCAATAGCCAGGCGCCTTGGCATTGC
TCCCAACACCGTAAACAAATATCTTAATAAGGAGTGGTGTCAAATGGCAAAAAGAGGTTCGAAACTCGATCCTTTCAAGGATTACATCGAGAAAAGGCTC
CAGGAGTATCCCGAACTTACAGCGACAGTGTTGTTTAAGGAATTGGTGGAGAGAGGTTATACCGGTAAACTGACAATACTCCGGATGTATGTGTCCTCAA
TAAGACCCAAGGGGAAACCTGAAATTGTTGTCAGATTCGAAACAGAACCTGGTAGACAATTTCAGGTTGATTGGGGAACGGGTACAACGGTTATTGCGGG
CGAAAAGACGACCGTTAAGTTCTTCATTATGGTGTTGAGCTATTCACGGATGCTGTACGCGGAGATAGTACCAGATGAAAAGCTTGAGACCTTGATCCAA
GCTCATCTGCATGCCTTTGAGTACTTTGGTGGTTATCCCTCAGAAGGTCTGTACGACAACATGAAAACCGTTGTAAAGAAGCTTCAGAAACAGAAGGAAT
ACAACGCCAGATTCATGGATTTTGCAAACTTCTACGGCTTTAAAGTGATTACTCACAGGCCATATAATCCAAAAGCCAAAGGAAAGGTCGAGAGGTTGGT
TCCTTACGTAAGGGAGAATATTCTTTACGGCCAGAGTTATTCGAGCCTAACGGAATTGAAGAACGTATTAAGAGATTGGTTAGCAATAGCAAACCAGCGA
CTCCATTCCGAATTAAAAGAAACCCCTCTCGAAAGATTCGAGAGGGAAAAGAATCACCTAAATGAGCTAAGTAAATTGTATCCCATTCGCAGATTAAATA
CAAGACTCGTAAGAAACAAAGGCCAGATAGTCTACAAGGAAAGGGCATATCATGTTGGTAAAAAATACGTAGGGGAAAGAGTCAATCTCCAGGTGGAAGG
TTCACTACTGAAGATATACAGCAACGATGAACTCATTACAGTACATCCATTGAAAGACCAGGTAGAAAAAAGGTCTTTGAGAGAATACCAGGCTCTTGTG
GGTGATGCGGTATGAGCTACGAAAAAGTACACAGCTTGTTGAAAGAACTGAAACTGGAAGGAATCTCCAGGGTATTGGATTCTTCTCTCCAAAACTGGAG
CAAAGGAGATAAAGACGTTCTCGAATTGATTGAAGAACTACTAATAGCCCAGAAGAAAGAAAACGAGAACAAGAAATACATAACAGCTCTCAGATACAGC
GGATTACCTTTCCACAAAACCCTGGAAGAATTCGATTTCAGTTTCCAACCCAGCATAGACAGAAAACAGGTAATGGAACTGAAGACCCTGAGATTCATGT
ACGAAAAAGAGAACGTGGTATTTCTTGGACCTCCTGGAGTAGGAAAAACACACCTGGCGGTAGGTCTTGGAATGGAAGCCCTAAGGGAAGGAAAGAAAAT
ATACTTCGTTAATGCAATAACGTTGGTGGATCGATTAAAGAAAGCCTTTGTTGAGAGACATCTGGAAAAAACCATTAGATTCTACAAATCCCTTGACCTA
CTAATAATAGATGAACTGGGATACCTGCCGCTGGATACAGAGGGAGCGAAGCTGTTTTTTGAACTGGTGAGCCAGAAATACGAGCAGGGAAGCATAATCC
TGACATCCAACAGAAGCTTTACCGAATGGGACAAGATATTTGAAGACGAAGTATTAGCAACAGCGGTATTGGACAGACTCTTACACCATTGTACGATAGT
CAATATTCGTGGAAAAAGTTACAGACTGAAAGAAAAACAGAAAACGGGGCTTGTTGGTATACAGACGACGATTGGTAGAACCGACAAACTACGCAAATCT
GAGCGATGAAATTACGCAATTTTGATCGATGTTTTTTTGCATTTTTGGTTGATGTTGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1104 bp	367 aa	112	1215	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLTEKQVFEIRILYREGLSKLAIARRLGIAPNTVNKYLNKEWCQMAKRGSKLDPFKDYIEKRLQEYPELTATVLFKELVERGYTGKLTILRMYVSSIRPK
GKPEIVVRFETEPGRQFQVDWGTGTTVIAGEKTTVKFFIMVLSYSRMLYAEIVPDEKLETLIQAHLHAFEYFGGYPSEGLYDNMKTVVKKLQKQKEYNAR
FMDFANFYGFKVITHRPYNPKAKGKVERLVPYVRENILYGQSYSSLTELKNVLRDWLAIANQRLHSELKETPLERFEREKNHLNELSKLYPIRRLNTRLV
RNKGQIVYKERAYHVGKKYVGERVNLQVEGSLLKIYSNDELITVHPLKDQVEKRSLREYQALVGDAV

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
798 bp	265 aa	1212	2009	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSYEKVHSLLKELKLEGISRVLDSSLQNWSKGDKDVLELIEELLIAQKKENENKKYITALRYSGLPFHKTLEEFDFSFQPSIDRKQVMELKTLRFMYEKE
NVVFLGPPGVGKTHLAVGLGMEALREGKKIYFVNAITLVDRLKKAFVERHLEKTIRFYKSLDLLIIDELGYLPLDTEGAKLFFELVSQKYEQGSIILTSN
RSFTEWDKIFEDEVLATAVLDRLLHHCTIVNIRGKSYRLKEKQKTGLVGIQTTIGRTDKLRKSER

Blast result :

Comments

ISKol10 is 53% (ORFA : the transposase) aa similar to ISMac3 and 70% (ORFB : istB, helper of transposition) to ISMac9.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G. and Noll,K. (2009) Direct submission GenBank.