ISAzba1
- Family ISKra4
- Group ISAzba1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| EU784144 | ND | Azospirillum brasilense | Azospirillum brasilense Sp245 85-MDa plasmid p85 |
DNA section
IS Length : 1465 bp
Ends
IR Length : 22/23
IRL : GAGACCGTCGCAAACCTGGGGGGAGAATGGTACGGCGCGCTCGGATGTCA
IRR : GAGACCGTCGCAAACTTGGGGGGCGTTACGCCGCCCGGCGAAATGGTCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTGCTAAAGGA | AGTCTCGTTC | 0 |
DNA sequence
GAGACCGTCGCAAACCTGGGGGGAGAATGGTACGGCGCGCTCGGATGTCAGCGAGTCGGGTGTACGATGAAGTGGCGGGTCATGGTTGAGGTGACGGGCG
AGGAGGGCGCGGTGACGCAGCATGTGATCTCCGAGGGTAAGCGCACCGGGGCCGGTCAGGCGGCGACGCTCGGGCTGAGTCTGAGCGAGGGCAAGGCGAC
CCTGGCCGGCCTGCAGCGCGTCCTGGTCATGGCCCAGGCCGACGCGCATTGTCGCCTTCGGCGTCGGTGCGGCCACTGCGGAGCGTCGCGCCCGCTCAAG
GACCACCGCTCCCGCCGGCTGGTGTCGTTGTTTGGCACGGTGGAGGTGCGCGCACCCCGTTTTAAGCCCTGTCGCTGCGGCGTGGCCTGCCGGCGCAGCC
TGACCCCGGTCGCCGAGATCATGCCCGACCGCTGCACCCCTGAGTACGAACGCATTCTATCCGGGATGGGCAGCGCGCTGCCCTATCGGCGGGCGCGGGC
GTTGCTGGCCGAGCTGCTGCCCTTGGATGCGGCTCCCGATGTGGAAACGGTCCGCCGGCGCACGCTCCAGGTCGGCGCCCGGCTCGAACGGGCGGCGCTG
GTCCCGCCGGACGAGGGTTCGTCGGCCCCGGCAACGGCGATCACGCTGGCCATCGACGCGGGGCATGTGAAGTCGATTAAGACTTATCAGGTGCGCTCGT
TCGAGGTGATGCTCGCGCGGGTGGGCAACGATCAGGGGCGGGAGGTCGTTTTCAGCAGCCTGCCGACCGAGGCCGACCGGCAAGTGCGGCAACTCCATCA
TGTTTTGCGCCGTCTGGGGGCGACGGCGACCACACCGGTCACCATCCTCAGCGACGGCGCAGAGGGTCCACGGTCGCTGGGCGCCGCGGCCAGCGTCGGC
CCGATCCGTAACGTCCTCGACTGGTTCCACCTATCGATGCGGGTCCAGCACGTGGCGCAGGCCGTCAAGGGCTGGGCCACGGCGGTGCCGGACGCCGCGG
AGCGGACGACGATGCTGGACGACGCCGTGACCCGCCTGCGTTGGCGGCTCTGGCACGGCCAGACCGAACGGGCGCTGACCTTGATCGGCGACCTCCTGAC
CGACCTCGACGCCGCTTTGGAAGCCGCTGACGACCCGCCGGCCCGGAAAGTGGCCGCGCTGCTGCGCGCCTTGGAGACGTACGTCGTCGGGCAAGCCGAC
CTGATCATCGACTACGCCGCCGCCCGGCATCGGGGCGAGGCCATTTCCACGGCGACGACCGAAAGCACCGTCCAACGTCTGCTGCACCGACGCATGGGCG
CCAATCAGCAGATGCGCTGGTCTCCGCGCGGTGCCCATCGGATGCTAAAGGTGCGCACCGCGGTGATGAACGGCACACTCGCCGCGGATCACGCCGCGGC
CGAGCCATACGCCCGCCGACCATTTCGCCGGGCGGCGTAACGCCCCCCAAGTTTGCGACGGTCTC
AGGAGGGCGCGGTGACGCAGCATGTGATCTCCGAGGGTAAGCGCACCGGGGCCGGTCAGGCGGCGACGCTCGGGCTGAGTCTGAGCGAGGGCAAGGCGAC
CCTGGCCGGCCTGCAGCGCGTCCTGGTCATGGCCCAGGCCGACGCGCATTGTCGCCTTCGGCGTCGGTGCGGCCACTGCGGAGCGTCGCGCCCGCTCAAG
GACCACCGCTCCCGCCGGCTGGTGTCGTTGTTTGGCACGGTGGAGGTGCGCGCACCCCGTTTTAAGCCCTGTCGCTGCGGCGTGGCCTGCCGGCGCAGCC
TGACCCCGGTCGCCGAGATCATGCCCGACCGCTGCACCCCTGAGTACGAACGCATTCTATCCGGGATGGGCAGCGCGCTGCCCTATCGGCGGGCGCGGGC
GTTGCTGGCCGAGCTGCTGCCCTTGGATGCGGCTCCCGATGTGGAAACGGTCCGCCGGCGCACGCTCCAGGTCGGCGCCCGGCTCGAACGGGCGGCGCTG
GTCCCGCCGGACGAGGGTTCGTCGGCCCCGGCAACGGCGATCACGCTGGCCATCGACGCGGGGCATGTGAAGTCGATTAAGACTTATCAGGTGCGCTCGT
TCGAGGTGATGCTCGCGCGGGTGGGCAACGATCAGGGGCGGGAGGTCGTTTTCAGCAGCCTGCCGACCGAGGCCGACCGGCAAGTGCGGCAACTCCATCA
TGTTTTGCGCCGTCTGGGGGCGACGGCGACCACACCGGTCACCATCCTCAGCGACGGCGCAGAGGGTCCACGGTCGCTGGGCGCCGCGGCCAGCGTCGGC
CCGATCCGTAACGTCCTCGACTGGTTCCACCTATCGATGCGGGTCCAGCACGTGGCGCAGGCCGTCAAGGGCTGGGCCACGGCGGTGCCGGACGCCGCGG
AGCGGACGACGATGCTGGACGACGCCGTGACCCGCCTGCGTTGGCGGCTCTGGCACGGCCAGACCGAACGGGCGCTGACCTTGATCGGCGACCTCCTGAC
CGACCTCGACGCCGCTTTGGAAGCCGCTGACGACCCGCCGGCCCGGAAAGTGGCCGCGCTGCTGCGCGCCTTGGAGACGTACGTCGTCGGGCAAGCCGAC
CTGATCATCGACTACGCCGCCGCCCGGCATCGGGGCGAGGCCATTTCCACGGCGACGACCGAAAGCACCGTCCAACGTCTGCTGCACCGACGCATGGGCG
CCAATCAGCAGATGCGCTGGTCTCCGCGCGGTGCCCATCGGATGCTAAAGGTGCGCACCGCGGTGATGAACGGCACACTCGCCGCGGATCACGCCGCGGC
CGAGCCATACGCCCGCCGACCATTTCGCCGGGCGGCGTAACGCCCCCCAAGTTTGCGACGGTCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1374 bp | 457 aa | 67 | 1440 | + | No |
Chemistry : DDE
ORF sequence :
MKWRVMVEVTGEEGAVTQHVISEGKRTGAGQAATLGLSLSEGKATLAGLQRVLVMAQADAHCRLRRRCGHCGASRPLKDHRSRRLVSLFGTVEVRAPRFK
PCRCGVACRRSLTPVAEIMPDRCTPEYERILSGMGSALPYRRARALLAELLPLDAAPDVETVRRRTLQVGARLERAALVPPDEGSSAPATAITLAIDAGH
VKSIKTYQVRSFEVMLARVGNDQGREVVFSSLPTEADRQVRQLHHVLRRLGATATTPVTILSDGAEGPRSLGAAASVGPIRNVLDWFHLSMRVQHVAQAV
KGWATAVPDAAERTTMLDDAVTRLRWRLWHGQTERALTLIGDLLTDLDAALEAADDPPARKVAALLRALETYVVGQADLIIDYAAARHRGEAISTATTES
TVQRLLHRRMGANQQMRWSPRGAHRMLKVRTAVMNGTLAADHAAAEPYARRPFRRAA
PCRCGVACRRSLTPVAEIMPDRCTPEYERILSGMGSALPYRRARALLAELLPLDAAPDVETVRRRTLQVGARLERAALVPPDEGSSAPATAITLAIDAGH
VKSIKTYQVRSFEVMLARVGNDQGREVVFSSLPTEADRQVRQLHHVLRRLGATATTPVTILSDGAEGPRSLGAAASVGPIRNVLDWFHLSMRVQHVAQAV
KGWATAVPDAAERTTMLDDAVTRLRWRLWHGQTERALTLIGDLLTDLDAALEAADDPPARKVAALLRALETYVVGQADLIIDYAAARHRGEAISTATTES
TVQRLLHRRMGANQQMRWSPRGAHRMLKVRTAVMNGTLAADHAAAEPYARRPFRRAA
Blast result :
Comments
ISAzba1 is 38% aa similar to ISTesp2. ISAzba1 was found to be involved in cointegrate formation between an Azospirillum brasilense Sp245 85-MDa plasmid (p85) and a suicide plasmid vector pJFF350 (Omegon with oriV from pBR322 + oriT + IS1 defective in transposition) which has been constructed by Fellay, R., Krisch, H.M., Prentki, P., and Frey, J. (Gene, 1989, 76, 215–226). The left and right flanks of the ISAzba1 insertion site refer to DNA of pJFF350 and p85, correspondingly.
References
1] Katsy, E.I. and Prilipov, A.G. (2008) Direct submission.
2] Katsy,E.I. and Prilipov,A.G. (2009) Plasmid 62 (1), 22-29
2] Katsy,E.I. and Prilipov,A.G. (2009) Plasmid 62 (1), 22-29