ISAzba3
- Family IS5
- Group IS903
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| EU595704 | ND | Azospirillum brasilense | Azospirillum brasilense Sp245 85-MDa plasmid p85 |
DNA section
IS Length : 1112 bp
Ends
IR Length : 18/22
IRL : GGCCTTGTTGCACAAATCCGGCCGGTGCCCCTCTGTTGGCCGGAGAGGCG
IRR : GGCGTTGTTGCGTAAATCCCGCTACGGACAGGCTTTGCGCCTTATCCGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATGCGCACT | TCAGGCAGCA | 0 |
DNA sequence
GGCCTTGTTGCACAAATCCGGCCGGTGCCCCTCTGTTGGCCGGAGAGGCGGTTGGGGTATAGAGGCGGGCGCTGAAGCCGAGAGCCCGCATGCCGTACAA
GCACAACGAACCACGTCGCCACAAAATCCCGAAGGCCAAATACAAAGTTGAAAACTGGCGGGAGTACAACCGGGCTCTGCGGCAGCGTGGCAGTTTGACG
GTGTGGATCTCGCCCGATGCCCTGGCCATGTGGACGCCGGCCCAGACCGGGCGGCGGGGCCGGCCGCCGGCTTACTCGGACCTCGCGATCGAAACCGGGA
TCATGCTGCGCTTGGCGATGGGCCGCCCATGGCGCCAGACCGAGGGCCTGCTGCGCTCGATCGTCAAGTTGCTCGGCTTGGACCTCCCCGTTCCCGATTA
CACGACGCTGGCCCGGCGCAGCGCCCGGCTTTCACGGACGACGGCGCTGAAGACCCCGACCGGGCCGGTCGACGTCGTCATCGACTCAACCGGTCTGAAG
ATTTCTGGAACCGGCGAGTGGCAGCACGAGAAGCACGGGGGCAGGCCGCGCCGGAGTTGGCGCCGATTGCACTTGGCCATCGATCCGGACAGCGGGGACA
TCCTCGCTGCCGAGCTGACCACCACGGACGACGGCGATGCCTCCCAAGTCGGCCCCCTGCTCGATCAGATCGCTGGCCCGGTCCGGGCCGTGCTGGCCGA
TGGCGCGTATGACGGCGAGCCGGTGTACCATGCCATCGCCGAACGCCACGCCGACGCCGCGGTGATCATTCCACCGCGCTCCACGGCGGTGCTGAGTGAC
ACCGCCACGACCGCCCCCACGCAGCGCGACCAGCATATCCAGTTGCTCGCCGAAAAGGGCCGCATCGGCTGGCAGACGGCCACCGGCTACGGAAAGCGCG
CGCTCGGCGAAACCGCCTTCTCCCGGTACAAAACTGTGATCGGACGCAGCCTGCGGGCCCGAACTCTGTCCGCGCAGAAGGTCGAAGCCCACATCGCCTG
CGCGGTCATCAACCGCATGACCAGCCTCGGCATGCCGGTCTCCGAAAAGGCCGCCTGAGAGCCGCGGATAAGGCGCAAAGCCTGTCCGTAGCGGGATTTA
CGCAACAACGCC
GCACAACGAACCACGTCGCCACAAAATCCCGAAGGCCAAATACAAAGTTGAAAACTGGCGGGAGTACAACCGGGCTCTGCGGCAGCGTGGCAGTTTGACG
GTGTGGATCTCGCCCGATGCCCTGGCCATGTGGACGCCGGCCCAGACCGGGCGGCGGGGCCGGCCGCCGGCTTACTCGGACCTCGCGATCGAAACCGGGA
TCATGCTGCGCTTGGCGATGGGCCGCCCATGGCGCCAGACCGAGGGCCTGCTGCGCTCGATCGTCAAGTTGCTCGGCTTGGACCTCCCCGTTCCCGATTA
CACGACGCTGGCCCGGCGCAGCGCCCGGCTTTCACGGACGACGGCGCTGAAGACCCCGACCGGGCCGGTCGACGTCGTCATCGACTCAACCGGTCTGAAG
ATTTCTGGAACCGGCGAGTGGCAGCACGAGAAGCACGGGGGCAGGCCGCGCCGGAGTTGGCGCCGATTGCACTTGGCCATCGATCCGGACAGCGGGGACA
TCCTCGCTGCCGAGCTGACCACCACGGACGACGGCGATGCCTCCCAAGTCGGCCCCCTGCTCGATCAGATCGCTGGCCCGGTCCGGGCCGTGCTGGCCGA
TGGCGCGTATGACGGCGAGCCGGTGTACCATGCCATCGCCGAACGCCACGCCGACGCCGCGGTGATCATTCCACCGCGCTCCACGGCGGTGCTGAGTGAC
ACCGCCACGACCGCCCCCACGCAGCGCGACCAGCATATCCAGTTGCTCGCCGAAAAGGGCCGCATCGGCTGGCAGACGGCCACCGGCTACGGAAAGCGCG
CGCTCGGCGAAACCGCCTTCTCCCGGTACAAAACTGTGATCGGACGCAGCCTGCGGGCCCGAACTCTGTCCGCGCAGAAGGTCGAAGCCCACATCGCCTG
CGCGGTCATCAACCGCATGACCAGCCTCGGCATGCCGGTCTCCGAAAAGGCCGCCTGAGAGCCGCGGATAAGGCGCAAAGCCTGTCCGTAGCGGGATTTA
CGCAACAACGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 969 bp | 322 aa | 90 | 1058 | + | No |
Chemistry : DDE
ORF sequence :
MPYKHNEPRRHKIPKAKYKVENWREYNRALRQRGSLTVWISPDALAMWTPAQTGRRGRPPAYSDLAIETGIMLRLAMGRPWRQTEGLLRSIVKLLGLDLP
VPDYTTLARRSARLSRTTALKTPTGPVDVVIDSTGLKISGTGEWQHEKHGGRPRRSWRRLHLAIDPDSGDILAAELTTTDDGDASQVGPLLDQIAGPVRA
VLADGAYDGEPVYHAIAERHADAAVIIPPRSTAVLSDTATTAPTQRDQHIQLLAEKGRIGWQTATGYGKRALGETAFSRYKTVIGRSLRARTLSAQKVEA
HIACAVINRMTSLGMPVSEKAA
VPDYTTLARRSARLSRTTALKTPTGPVDVVIDSTGLKISGTGEWQHEKHGGRPRRSWRRLHLAIDPDSGDILAAELTTTDDGDASQVGPLLDQIAGPVRA
VLADGAYDGEPVYHAIAERHADAAVIIPPRSTAVLSDTATTAPTQRDQHIQLLAEKGRIGWQTATGYGKRALGETAFSRYKTVIGRSLRARTLSAQKVEA
HIACAVINRMTSLGMPVSEKAA
Blast result :
Comments
ISAzba3 is 81% aa similar to ISAli17. ISAzba3 was found to be involved in cointegrate formation between an Azospirillum brasilense Sp245 85-MDa plasmid (p85) and a suicide plasmid vector pJFF350 (Omegon with oriV from pBR322 + oriT + IS1 defective in transposition) which has been constructed by Fellay, R., Krisch, H.M., Prentki, P., and Frey, J. (Gene, 1989, 76, 215–226). The left and right borders of the ISAzba3 insertion site refer to DNA of p85 and pJFF350, correspondingly.
References
1] Katsy, E.I. and Prilipov, A.G. (2008) Direct submission.
2] Katsy,E.I. and Prilipov,A.G. (2009) Plasmid 62 (1), 22-29
2] Katsy,E.I. and Prilipov,A.G. (2009) Plasmid 62 (1), 22-29