ISfinder

ISNpu10

Family ISAzo13
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010631	ND	Nostoc punctiforme	Nostoc punctiforme PCC 73102 Nostoc punctiforme PCC 73102 plasmid pNPUN01

DNA section

IS Length : 1334 bp

Ends

IR Length : 29/35

IRL : GGCATCATGTAAAAATAACTTGAACAATTTGCTGAATGGTAAGATGATCA
IRR : GGAGTTATGTAAAAATAAATTGAACAATTAACTGACTACTTTGGGTTTGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACCGTTTGAGCAATTAG	TAA	GTATTGCCACTGACCTG	3
ATTCATAAAGAAGTTGC	TAA	CAAGGCAGACGAGGGAA	3
GCAAACCCCCTTTACCA	TTG	GCATTGGGACTCAGGGT	3
AGCATAGCTGCCTTTTC	TTA	GTCACATTCAACTATGA	3
AACTCAAGGTGCAACCC	TAA	GAGTTTATCTAGAAAGC	3
TCTGTGTAGCGATCGCT	TAA	CAGTTATTTACCTATTC	3
GCGTAAGACCTATATAC	TTA	ACTATCTTTTAGAAAAA	3
TAAAAGGTGAAGTTCCT	TTA	GCATCTGATTGCAAGTA	3
GCCAGTGCCAAACTCAC	CTA	GTATTGAGATATGCTCT	3
CTTTTTTCCCTCCTTTC	TAA	GGGGGGATTAAGGGGGG	3
GCTACTTCAGCCTGCTG	TAA	CTGAAGTAAAAATGCTT	3
TAGTGTCAGCACGAGTC	TAA	CCCAATAGAACGCTTGC	3

DNA sequence

GGCATCATGTAAAAATAACTTGAACAATTTGCTGAATGGTAAGATGATCAGAATGTAACCGAATTAAAGGCTTATATGTCTGATAAGCAGGTAGTAGAAA
GCATTCAAGACAAGTACGATTCGTTATCGCCTTATTTGAATGAGAAAACACGGCGTATTTGGGCAGCAATTGAAGCCCGAAGCCTGGGCTGGGGAGGCGT
GAGTCAGGTTGCGCTCGCAACTGGACTATCCCGGACTACAATCCATGCTGGGATACGGTTATTGTTAGACGCTTCGGGGGAAAAAACCTCGAATGATGAT
AGTAATCGAATTCGTTCGTCAGGTGCTGGACGTAAACTACTTGAAGAAAAAGACGCAATGCTGCTATCAGATTTAGAATCGCTGATTGAACCAGTGACAC
TGGGAGACCCAGAATCTCCTCTAAAATGGACTTCTAAAAGTGTTGTGAAACTGGCTGCGGCATTAAACATTGGGGGACATAGGACTAGTCCTAAAAGTGT
TTATAACTTACTTGAATCGCTTGGCTACAGCTTACAATCAAATCGTAAAACCCGTGATGGCTCATCTCATCCAGATAGAGATGATCAGTTTTTACATATT
TCCAACCAAGTCTTGCACTTTCAATCCCAGAACGAACCCGTAATTTCAGTTGATACAAAAAAAAAGAATTAATTGGAGATTTTAAAAATTCTGGAACCGA
GTGGTGTGAAAAGGAACAGCCAATTGAGGTGAAAATGCATGATTTTGTTGACCCCAAGTTGGGCAAGGCAATTCCCTACGGAATTTATGACTTAACCTCA
AATCAAGGATGGGTAAATGTTGGCATTGACCACGATACCGCAGAGTTTGCAGTCGAGTCTATTCGTCATTGGTGGTACTCAATGGGTAAACAAGTTTATC
CCAGCAGTGAGCATATAATGATTACGGCTGATTGCGGTGGTAGCAATAGTTATCGCTCACGATTGTGGAAATTGAAGTTACAAGAATTAGCAACTGATAC
TGGTAAAACTATTCATGTGTGTCATTTTCCTCCAGGCACAAGTAAATGGAATAAGATTGAGCATCGCTTGTTTTGTCACATTACCCAAAACTGGCGAGGC
AGACCATTAACTAGCTTGCAAGTTGTGATTAATCTAATTCGCAATACTACCACCACACAAGGATTAGAAGTTGAAGCTAGATTAGATCCAAATCTCTACA
AAACGGGAATCAAGGTTACAGACCAAGAGCTTGATACTATCGCAATCGAACGAAATTCTTTTCATGGTGAGTGGAACTATATTATCAAACCCAAAGTAGT
CAGTTAATTGTTCAATTTATTTTTACATAACTCC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
597 bp	198 aa	76	672	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSDKQVVESIQDKYDSLSPYLNEKTRRIWAAIEARSLGWGGVSQVALATGLSRTTIHAGIRLLLDASGEKTSNDDSNRIRSSGAGRKLLEEKDAMLLSDL
ESLIEPVTLGDPESPLKWTSKSVVKLAAALNIGGHRTSPKSVYNLLESLGYSLQSNRKTRDGSSHPDRDDQFLHISNQVLHFQSQNEPVISVDTKKKN

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
654 bp	217 aa	654	1307	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

YKKKELIGDFKNSGTEWCEKEQPIEVKMHDFVDPKLGKAIPYGIYDLTSNQGWVNVGIDHDTAEFAVESIRHWWYSMGKQVYPSSEHIMITADCGGSNSY
RSRLWKLKLQELATDTGKTIHVCHFPPGTSKWNKIEHRLFCHITQNWRGRPLTSLQVVINLIRNTTTTQGLEVEARLDPNLYKTGIKVTDQELDTIAIER
NSFHGEWNYIIKPKVVS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1232 bp	410 aa	76	1307	+	Yes

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MSDKQVVESIQDKYDSLSPYLNEKTRRIWAAIEARSLGWGGVSQVALATGLSRTTIHAGIRLLLDASGEKTSNDDSNRIRSSGAGRKLLEEKDAMLLSDL
ESLIEPVTLGDPESPLKWTSKSVVKLAAALNIGGHRTSPKSVYNLLESLGYSLQSNRKTRDGSSHPDRDDQFLHISNQVLHFQSQNEPVISVDTKKKELI
GDFKNSGTEWCEKEQPIEVKMHDFVDPKLGKAIPYGIYDLTSNQGWVNVGIDHDTAEFAVESIRHWWYSMGKQVYPSSEHIMITADCGGSNSYRSRLWKL
KLQELATDTGKTIHVCHFPPGTSKWNKIEHRLFCHITQNWRGRPLTSLQVVINLIRNTTTTQGLEVEARLDPNLYKTGIKVTDQELDTIAIERNSFHGEW
NYIIKPKVVS

Blast result :

Comments

ISNpu10 is 65% aa similar to ISArch16. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2008)
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M. and Atlas,R. (2008) Direct submission GenBank.