ISfinder

ISNha7

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007964	ND	Nitrobacter hamburgensis	Nitrobacter hamburgensis X14

DNA section

IS Length : 1302 bp

Ends

IR Length : 19/20

IRL : CAGGCTGTTGAAGAAGTCTCTGGCGCAGCGGTTCTGGGCATGATTCTCTT
IRR : CAGGCTGTTGAAGAAGTTTCCCACGGAGACGGATATGGAATGGAGTCAGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCTCCGAAAG	CTAG	AGCGTTTTCG	4
TAACCTTGCT	CTAG	AGCCTTTCCG	4
TCGAAACTCC	CTAG	CGCTTGGTGC	4
ACCCGAGGCT	CTAG	AGCCTTTCCG	4
GCGAAAAGCT	TTAG	AGCCGCGTTT	4
AAGTCATCAT	TTAG	AGCGTTTTCG	4
CGGATTCCAC	TTAG	AGCGTTTTCG	4
ATTTCTGAAC	CTAG	AGCCTTTTCG	4
GGCTTGCGAG	CTAG	AGCGTTTTCG	4
CGAAAAGGCT	CTAG	GCCCTTTTTT	4
CGAAAAGGCT	CTAG	TGCGACATGA	4
CGAAAAGGCT	CTAG	GCCTCGCGAT	4
ATCATGTGGC	CTAG	AGATTGGCGC	4
CGAAAACGCT	CTAG	TTGTCGTCGC	4
CAGGCGTAGC	CTAG	AGCGTTTTCG	4
CGGAAAGGCT	CTAG	GCGTAGGCGA	4
CGGCCTGAAC	CTAG	AGCCTTTCCG	4
CGAAAACGCT	CTAG	AACTGAATCC	4
TGCGTTAATC	CTAG	AGCGTTTTCG	4
ATGGCGAGCCCTAG		CTAATGCTTAATATCAC	0
GCCGTCGCGC	CTAG	AGCCTTTCCG	4

DNA sequence

CAGGCTGTTGAAGAAGTCTCTGGCGCAGCGGTTCTGGGCATGATTCTCTTGATGCGGATGCGTCGAGGGGGGAGCGATGCGGGGAAGCGACGAACGGTCA
GGCTCGCTGTTCAGCTATGTGGACTTGGAGGCTCGGATTCGCTCCGACCATCCGCTGCGAACGATCCGACAGATCGCGAACGCGGCGTTGAATGATCTGT
CGAGGGACTTTGACAAGCTCTACACGGCGTTCGGCCGTCCCTCGATCGCACCGGAGAAGCTGCTTCGGGCAATGCTGCTGCAGGCATTCTACGGGATCCG
CTCGGAACGGCAGTTGATGGAGCGGCTGGAGTTCGACCTGCTGTTGCGCTGGTTCGTGGGCTTGGGCGTGGACGACCCGGTGTGGGACCACTCGACCTTC
TCGAAGAACCGCGACCGATTGCTTGAAGGTGAGATCGCCGCGAAGTTCTTGAACGCGCTGATGGGGCAGCACCAGGTCAAGCGGCTGTTATCAAGCGAGC
ATTTTTCGGTCGACGGCACGCTGATCGAGGCGTGGGCATCGATCAAGAGCTTCCGGCGCAAGGACGGCGGTGACCAGGACAGTGATGGACCGGGACGCAA
CGCCGAGCGCAGTTTCCACAACGAGAAGCGCTGCAACGAGACGCATCAGAGCACGACCGATCCCGAGGCACGGCTCTATAAGAAGGGCGGCGGCCAGCCG
GCGAAGCTTTGCTACATCGGCCATGCCCTGATGGAGAACCGCAACGGACTGGCGGTGCTGGGTGGCGTGAGCCGGGCGACCGGAACGGCGGAGCGGGATC
AGGCGTTGGCGCTGATCGACTGCCACCGCGGCCAAAGCGAGCGGCGGATCACGCTGGGCGCCGACAAGGCCTATGACGTCACCGCATTCGTCGAGGACTT
AAGACGGCGTTCGGTCACGCCGCACATCGCCATCGACGGGCATTTGAGCAAGACCGGAAAGCCGCGCAAGACCGCGATCGACCAGAGGACTCTCCGTCAT
GCCGGATATGCCGTCAGCCAACGCTGTCGCAAGCGCATCGAGGAGGTGTTCGGCTGGATCAAGGCCTCCGCCGGACTTGCCAAGATCAAGCTGCGAGGCC
GCGACCGCGTCAACGCCACCTTCACCCTGGCGCTGGCGGCCTACAACCTGATCCGCTTGCCCAAACTCCTGGCAGCCGCCGCGTGAAACACAAGTCGTGC
ACCGTCAAGATGCTCGATCAGGACCCTGATGGTGGCGCCGCCCGCAGATCACGCTGACTCCATTCCATATCCGTCTCCGTGGGAAACTTCTTCAACAGCC
TG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1110 bp	369 aa	77	1186	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGSDERSGSLFSYVDLEARIRSDHPLRTIRQIANAALNDLSRDFDKLYTAFGRPSIAPEKLLRAMLLQAFYGIRSERQLMERLEFDLLLRWFVGLGVDD
PVWDHSTFSKNRDRLLEGEIAAKFLNALMGQHQVKRLLSSEHFSVDGTLIEAWASIKSFRRKDGGDQDSDGPGRNAERSFHNEKRCNETHQSTTDPEARL
YKKGGGQPAKLCYIGHALMENRNGLAVLGGVSRATGTAERDQALALIDCHRGQSERRITLGADKAYDVTAFVEDLRRRSVTPHIAIDGHLSKTGKPRKTA
IDQRTLRHAGYAVSQRCRKRIEEVFGWIKASAGLAKIKLRGRDRVNATFTLALAAYNLIRLPKLLAAAA

Blast result :

Comments

ISNha7 is 75% aa similar to ISRsp5.

References

1] ISfinder annotation (2010)
2] Starkenburg,S.R., Larimer,F.W., Stein,L.Y., Klotz,M.G., Chain,P.S., Sayavedra-Soto,L.A., Poret-Peterson,A.T., Gentry,M.E., Arp,D.J., Ward,B. and Bottomley,P.J. (2008) Appl. Environ. Microbiol. 74 (9), 2852-2863.