ISfinder

ISVba2

Family IS5
Group IS903

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAZW01000002	ND	Vibrionales bacterium	Vibrionales bacterium SWAT-3

DNA section

IS Length : 1054 bp

Ends

IR Length : 19

IRL : GGCTTTGTTGCGTAATTCATTGGAACTAAACCTAAAACCTACGATCTGAT
IRR : GGCTTTGTTGCGTAATTCAGTTTAGAGACAGAGCAGCCCCATTTCGTGCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCTCGTTTGT		TTGAAGATAG	0
TTGCTGAACA		TTTCGGATAG	0

DNA sequence

GGCTTTGTTGCGTAATTCATTGGAACTAAACCTAAAACCTACGATCTGATCAGCTATATTCACTCCCTCTCGTAGCCCTATGCCTAAACCTCGTTACAAA
ACAACCAACTGGAAGCAATACAACCAATCACTCATTAACCGCGGTTCTTTGACCTTTTGGATTGATGAAGAAGCGATAAGTGGCTGGACGCAAAGCAAAC
AGAATAAGCGCGGGAGGCCTCGTCGGTTCAGTGATTTAGCTATCACGACAGCCCTCATGGTGAAACGAGTTTTTTCTATGCCATTAAGAGCGCTGCAAGG
TTTTATCAACTCGATATTTAGGTTAGCCCATGTACCGTTAAGTTGTCCGCATTACACCTGCATCAGTCGTAGAGCCAAGCAAGTTGAGGTCTCACTTAAG
ACAAAAACGAGAGGTACGATACAACATCTAGCTATTGATGCGACTGGCCTTAAGGCTTATGGCGAAGGTGAATGGAAAGTCAAAAAACACGGGACAGATG
GCAAACGTAGAGTCTGGCGAAAACTTCATATTGCCGTGGATACAAACACTCATGAAATTATTGCAGCCGAGCTAAGTCTATCGACAGTTACAGATGGAGA
AGTACTCCCGAATTTACTGAAACAAACTCGCCGAAGTATCCACGAGGTGTCTGGTGATGGCGCTTATGATACGAGAGCATGTCACGATGCAATTAAGATT
AAGCGAGCCGTTGCGCTTATTCCTCCAAGAGAAGGGGCTGCCTTCTGGGAGCGGGGTCATCCCCGAAATCTCGCAGTAGGTTGCCAGAAGTTATATGGCT
CAAATAAGTATTGGAAAGAGCGGTATGGTTACCACAAACGCTCCCTGTCAAAAACAGCGATGTATCGAGTTAAACAGTTACTTGGTGGTCGTTTAAGTTT
AAGAAATTACAATGCACAAGTGGGAGAAACTTACGCGATGATAAAAGCGTTGAACAAGCTCACTGGGTTAGGTATGCCTGAAACTTGCCGTATTGACTAA
GAAATGCACGAAATGGGGCTGCTCTGTCTCTAAACTGAATTACGCAACAAAGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
921 bp	306 aa	80	1000	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKPRYKTTNWKQYNQSLINRGSLTFWIDEEAISGWTQSKQNKRGRPRRFSDLAITTALMVKRVFSMPLRALQGFINSIFRLAHVPLSCPHYTCISRRAK
QVEVSLKTKTRGTIQHLAIDATGLKAYGEGEWKVKKHGTDGKRRVWRKLHIAVDTNTHEIIAAELSLSTVTDGEVLPNLLKQTRRSIHEVSGDGAYDTRA
CHDAIKIKRAVALIPPREGAAFWERGHPRNLAVGCQKLYGSNKYWKERYGYHKRSLSKTAMYRVKQLLGGRLSLRNYNAQVGETYAMIKALNKLTGLGMP
ETCRID

Blast result :

Comments

ISVba2 is 94% aa similar to ISVch8.

References

1] ISfinder annotation (2010)
2] Azam,F., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.