ISfinder

ISKra1

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
ADVG01000004	ND	Ktedonobacter racemifer	Ktedonobacter racemifer DSM 44963

DNA section

IS Length : 1391 bp

Ends

IR Length : 16

IRL : GTGCCTATCCGAATAATCTGCTATACTGAGGGAAGGATAAAAAAGACAAC
IRR : GTGCCTATCCGAATAACTCAGAGGCTTAAAAAGTCATCAAACCACAAGCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCAGGCACAC	TTA	GTATTCTCAA	3
GTGGTATTAA		TTAGTCCTCT	0

DNA sequence

GTGCCTATCCGAATAATCTGCTATACTGAGGGAAGGATAAAAAAGACAACCAGCAAGGAGAGCGAGAACGAACGCCGAATAATCGCACCACGACGGGATT
CCGGATTTCTGATGAGCTGTGGGCTGTGCTCCAGCCGCTGCTGCCAGTCCACGTCAATACGCATTGCTTTGGCGGGGGACGACCTCGCATCCCTGATCGA
CATTGTGCTGAAGCAATCTTCTACGTTTTGCGCACGGGCTGTCAATGGCAAGCTCTCGACCAAACCGAACTGTGTGCCCATTCGACGGCCCACGATCGGT
TTCAAGTCTGGGTAGAAGCGGGAGTTTTCTTGAAACTCTGGCAAACAGGGTTGGAGCAGTTTGATGAGCTCTGCGGGATTGAGTGGGACTGGCTCGCGAT
GGACGGAGCGATGACGAAAGCGCCGCTGGGTGGGGACAAAACAGGACCCAATCCTACCGACCGGGGTAAGTCCGGCGTCAAGCGCAGCCTGCTGACAGAG
GGCCATGGAGTGCCGATTGGCCTGACGGTTGAAGGAGCCAATCGACATGATATGAAACTGGTGCGAGCGACTATCGAGAGTTTGGTGGTCGTGCGTCCGG
AGCCCATAAAGGAGCGCCTACAGGGAATGTGCCTGGACAAAGGATACGATTATGATGAGGTCTATGCGATCTTGCAGGAGTTTGGCTTTACTGCTCATGT
ACGCTCTCGTGGAGAAGAAGCTAGGGCAATCAAGCGGGAAGCAGGCTTCAAAGCACGTCGCTGGGTAGTGGAACGTGCACATTCGTGGATGAACCGTTTT
CGCCGCTTACTTATTCGGTGGGAGAAAAAGCCTGCAAACTATCTGGCGTTTCTCCACTTTGCCTGTGGCTTGATTGCTTTTCGTGCCGCAGGGTTACTTT
AAAGAAGCTTTGTGCGCGAAGAGGAGAAAACAAATGCATCTTGACCAACCAGTCCAACGCGAGATCCTTCTCGTCCAGGAGCAGTTTTGGAAGGCCCTGA
AGACCAGAGATGCTTCCTTATTGGCAACGATTCTCGCACCAACGTTTGTCGGGCGTTCGCCAGGAGAACCCGATCAGACAAGGGGAGAGTTCATCACCAT
CCTCACCGCCTTTCCCATCTCGATCAGTGAAATTGCGGGTGAAGCGATTGAAGTGCATGTTTTTGGAGAAGTTGCTGTCCTCACAGGTGTTCAAGTTGCA
CAACTTCATCTTCCAGAAGGGAGGGTGAGGAGCAGTCGCGTCATGTTGAGCAATGTTTTTCGCCAGGAAGACCTGAGTTGGCGTATGGTGCTGTCCCATT
CATTTGAGATTGTGCAAGACCTCTAGGTGAAACCTCTGTTTTGCTTGTGGTTTGATGACTTTTTAAGCCTCTGAGTTATTCGGATAGGCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
777 bp	258 aa	126	902	+	No

ORF function : Transposase
Description :

ORF sequence :

MLQPLLPVHVNTHCFGGGRPRIPDRHCAEAIFYVLRTGCQWQALDQTELCAHSTAHDRFQVWVEAGVFLKLWQTGLEQFDELCGIEWDWLAMDGAMTKAP
LGGDKTGPNPTDRGKSGVKRSLLTEGHGVPIGLTVEGANRHDMKLVRATIESLVVVRPEPIKERLQGMCLDKGYDYDEVYAILQEFGFTAHVRSRGEEAR
AIKREAGFKARRWVVERAHSWMNRFRRLLIRWEKKPANYLAFLHFACGLIAFRAAGLL

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
393 bp	130 aa	934	1326	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MHLDQPVQREILLVQEQFWKALKTRDASLLATILAPTFVGRSPGEPDQTRGEFITILTAFPISISEIAGEAIEVHVFGEVAVLTGVQVAQLHLPEGRVRS
SRVMLSNVFRQEDLSWRMVLSHSFEIVQDL

Blast result :

Comments

ISKra1 is 75% (ORFA : the transposase) aa similar to ISAzo38.
The second ORF is a passenger gene annotated as hypothetical protein.

References

1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,J., Schneider,S., Sikorski,J., Klenk,H.-P. and Eisen,J.A. (2010) Direct submission GenBank.