ISfinder

ISAzs2

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISazl08_01

Accession number	Transposition	Origin	Host
NC_013854	ND	Azospirillum sp.	Azospirillum sp. B510 plasmid pAB510b Azospirillum sp. B510 plasmid pAB510f Azospirillum sp. B510 plasmid pAB510a Azospirillum sp. B510 Azospirillum sp. B510 plasmid pAB510c Azospirillum sp. B510 plasmid pAB510e

DNA section

IS Length : 2609 bp

Ends

IR Length : 34/47

IRL : TGCATATTTCGGCGGAAAGCACCCAGCGGGAACGGCGGAAAGCGCCCGGG
IRR : TGCGTATTTCGGCGAATTCGCCCAGGCGTTTCGGTTGAAAGCGCCCACCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCGACTGGCG	GGCACC	GACCGATGGC	6
TCCCGGAGAC	GGCGCC	GCGGGAGTAC	6
TCGCCGCGCA	GAGGTGCC	TGAGGCGCCT	8
CGTCAGCCAA	GTCATACT	GTCGGCGACG	8
GTGGATCGAG	GCCAAT	CGGAAGAAGA	6
CGCCCACCGG	ACGCAGGG	CGCCGCCGCA	8
GATGGGATTC		ACCCTCGAAG	0
TCGCCGCTTG	GCTCTGGC	TGACGCCGAA	8
GGCGCTGGAC	GGCTTG	CAGCCGGACGTCGGGCATGAGG	6
TGGTTCCATGCCCTGAC		GGCGGACCGC	0
CGGCCTGCCG	CACACC	CGCCAGACCG	6
GATGCAGGTT		CCCTGACCGAAGATGGC	0
AACGCGCTCG	CCTATG	TGCCCAAGGCT	6
AGGTGGAGAA		ATCGGCGCCG	0
GCGCCGGTCG	GGGCGA	CATGGGCGTT	6
AGACGGTATC	GGCAGC	GAGGCGAGAG	6
CCACCGCGCG	GGAATG	CGCTCGGCCG	6
CCACCTGATC	CTTAAACC	AAAGCGTCAG	8
CTACATGGAT		GTCCGATGTC	0

DNA sequence

TGCATATTTCGGCGGAAAGCACCCAGCGGGAACGGCGGAAAGCGCCCGGGCAGAACGGTGAAAGCGCCCAGCCGTTTCGGTGAATTCGCCCACCCGTAGG
AGTGGTGGTATCGGGGTCCGAACCAGCCTTTGGCATCCCCATGATCTTTCCACGGCACAGGCCAAGGAGGATCGGGATGCCCCGAGCGAGGTCGGACATG
CGGCGGATCAGAGAAGTCCTTCGTCTGCGGGATGAGTTTGGCGCCAGCCAACGGCAGATTGCCGATGCCTGCCGGCTGCCGCGCAGCACCGTGCGCGATT
ACCTGGAGCGGCTACGGGCCTCCGGGCTGCAGTACGCGGATGTGCTGGGCTGGACGGACGTCGAGCTGGAGGAGCGGCTGTTCCCGCCTCCGGTCACGTC
GGCGCGCCCGGTGCCCGACTGGCGGCACATCAGCCGGGAACTCGGCCGCCGTGGCGTAACGCTGCGGCTGCTGTGGGAGGAATACCTGGAGGTCCATCCC
GGCGGCTATCGCTACACCCAATTCGTCCAGCATTTCCGGGCATGGCAGGGTGCTCATGCCGAGCCGCGTCTGCGCCGGGAACATCGGCCGGGGGCGGCGA
TCGAGGTTGATTACGCTGGGATGACGCTGACCGTCGGGCTCGGCGCTGAGGCGCGGCAGGCCCAGGTGTTTGTCGCCTGCCTGCCGTATTCGGGCTACGT
CTATGCCGAGGCGACCTGGACCCAGCAGGCGGAGGAGTGGCTGGCCTCCCACACCCGGCTGTTCGAACATCTGGGCGGCGTGCCGGGCAAGCTGGTGCCG
GATAACCTCAAGGTCGGCGTCAGCCACGCCTCCTTCTACGATCCGGCGATCAACCCGGCTTATCACGATCTCGCCCGGCACTACCGCACCGCCGTCCTGC
CGGCCCGGGTGCGGCGCCCGCGCGACAAGCCCAGCGCCGAGAACGGCGTGCAGCAGGTCGAGCGGCGGGTGCTGGCCCCGCTGCGCGATACGCCCTTCGC
CACGCTGGACGCCGCCAACGCGGCCTTGCGCGACAAGCTGGCCACCCTCAATGCCGCTCCCTTGAGCCGCCGGCCGCAGGACACGCGCGCCGGCCTGTTC
GCCGCCGAGGAGCAGCCGACCCTGCGCCCCTTGCCGCCGGACCGCTTCGTGCCGGGCACCTGGGCGCGCCACAAGGTCCCGCCCGACTATCATCTCGCTC
TCGACGGCGGCGTCTACTCGGTGCCCCACACGCTGATCGGCAAGACGGTCGACGTGCACAGCACCGCCGGCGTGATCAGCGTCTTCCTGCGCGGCAAGCG
GATGGCCTGCCATGTCCGCCGGCAGGACGGCGCCACCGTGACGCTGGACGCCCATCGCCCCGCCAACCACCGGGCCGTCGCCCGCTTCACCCCCGATGCC
ATCCAGACCGAACTGGCCGCCATCGGCCCGGCCGCCGCGCTGCTGTTCGAACGCATCCTGGCCGGCGCCGATCACCCCGAACAGGCGGTGCGGGCCGGGA
TCGGCCTGATCCGCTTGGCGGCCACCCACGGCACCAGCCGGCTGGAGCAAGCCTGCCAGGCGGCGCTGGAGGCCAACGTCGGATCCTACCGCTACGTCCA
GCGCTGGTTGACCGCTCCCCCGGCCACAACGGCGGACGCGGCCGGTGCCGGCGAGCACACCAATCTGCGCGGCCCTTCCTACTATCACTGACGGAGATAC
GATGATGAAGCACGTCAACCACGAGCGGCTGCGCCAGTTGCGGCTGTACGGCATGGCCAAGGGGTTGGAGGCGCTGGAACGCCTGCCCGATCGCGGCCAA
CTGGCCTTCGACGAGCAGTTGGGCACGCTGATCGAGCGGGAAGCGGCAGAGCGCGCCAACACCGCACTCGCCAGCCGACTGAAACGTGCCCGGCTGCGCC
AGACGGCCTGCCTGGAGGACCTCGACCTGCGCACCCCGCGTGGGCTCGATCGCGGTGTTGTGCGCGAGCTGGCCACCGGGCGCTGGGTGAAGGAGAACCG
GCCGGTTCTGATCACCGGCCCGACCGGGATCGGCAAGACCTGGCTGGCCTGTGCACTCGGCAACCAGGCGGCGCGGGAAGGCCACAGCGTACTCTACACC
CGGCTGACCCGCCTGCTGGACGATCTGGCCACCGCCCGCCTGGACGGTTCGCTCGCCCGGCTGCTGCGACGGATCGCCCGGCTCGACCTGCTGATCCTGG
ATGACTGGGCGATGACCGAGCTGACCGCGCCTCAGCGCCTGGACCTGATGGAGGTGATCGATGACCGCCACGACCGGGCCGCAACCATGCTGGCCACCCA
AGTTCCCGTGGCCAACTGGCATCGGCTCATCGGCGATGCCACCTACGCCGACGCCATCCTCGACCGCCTCGTGCATCGGGCCTACCGCATCGACCTGCAT
GGCGACTCCATGCGCCGCACCAAGGCCGATGCCGCCAGCGAAACCCCCGACACCTAAGGCCGGTGCTTGGCAACCAAACCCACAACCTTCATAACGTAAA
TCCAGGGTTCGGCCCCGGCTCCCACCAGCTTGCAATTCGGGTGGGCGCTTTCGCCGAAACGGTGGGCGCTTTCAACCGAAACGCCTGGGCGAATTCGCCG
AAATACGCA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1515 bp	504 aa	177	1691	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRARSDMRRIREVLRLRDEFGASQRQIADACRLPRSTVRDYLERLRASGLQYADVLGWTDVELEERLFPPPVTSARPVPDWRHISRELGRRGVTLRLLW
EEYLEVHPGGYRYTQFVQHFRAWQGAHAEPRLRREHRPGAAIEVDYAGMTLTVGLGAEARQAQVFVACLPYSGYVYAEATWTQQAEEWLASHTRLFEHLG
GVPGKLVPDNLKVGVSHASFYDPAINPAYHDLARHYRTAVLPARVRRPRDKPSAENGVQQVERRVLAPLRDTPFATLDAANAALRDKLATLNAAPLSRRP
QDTRAGLFAAEEQPTLRPLPPDRFVPGTWARHKVPPDYHLALDGGVYSVPHTLIGKTVDVHSTAGVISVFLRGKRMACHVRRQDGATVTLDAHRPANHRA
VARFTPDAIQTELAAIGPAAALLFERILAGADHPEQAVRAGIGLIRLAATHGTSRLEQACQAALEANVGSYRYVQRWLTAPPATTADAAGAGEHTNLRGP
SYYH

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
756 bp	251 aa	1702	2457	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MMKHVNHERLRQLRLYGMAKGLEALERLPDRGQLAFDEQLGTLIEREAAERANTALASRLKRARLRQTACLEDLDLRTPRGLDRGVVRELATGRWVKENR
PVLITGPTGIGKTWLACALGNQAAREGHSVLYTRLTRLLDDLATARLDGSLARLLRRIARLDLLILDDWAMTELTAPQRLDLMEVIDDRHDRAATMLATQ
VPVANWHRLIGDATYADAILDRLVHRAYRIDLHGDSMRRTKADAASETPDT

Blast result :

Comments

ISAzs2 is 58% (ORFA : the transposase) and 73% (ORFB : the helper of transposition) aa similar to ISSpu5.

References

1] Isfinder annotation (2010)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.