ISAba15

  • Family IS5
  • Group IS1031
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
JN187417 ND Acinetobacter baumannii
Acinetobacter baumannii B0707-070
DNA section
IS Length : 873 bp

Ends


IR Length : 14/15

IRL : GAACCTGTTCACGATTAAAAAATATGCTAGGATAAGCCACATAATCGATT
IRR : GAACCTGTTCACAATCTTTTCAGCAACAAAGCGATGAAAGCAAGATTGGT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GAACCTGTTCACGATTAAAAAATATGCTAGGATAAGCCACATAATCGATTAATAACCGAATAATTCACCATGAGAAAAGCCTATCCAAGCGACATTAGCC
GAAAACAATTTGAGCACATACTACCCATTCTAGAAAGTGCAAGGAAAAAGACCAAACCAAGAACGGTTGACCTTTATGACGTATTTTGTGGGTTATTGTA
TGTACTAAGAACAGGTTGCCAATGGCGACAGCTGCCCCATGACTTTCCCAAATGGCGCACCGTCCACTCGTACTTTCAGAAATGGAGCGAGCTTGACGAA
GAAGGCAACAGCATTCTTGACCAAGCCTTAAAAAAAATTGGTCAAAAAAGCGCGAAAGAAGGACAAACGTAAGAGCAAAACCAGTTTTATCATCATTGAT
GCTCAAAGCGTAAAAAATACCGACACGGCTAAACACAAAGGCTATGACGCAGGAAAGAAAGTATCAGGGATTAAGCGCCATATTGCGGTAGATAGCCAAG
GTTTGCCGCATGCTATCTGTATTACCACTGCCAATATCACCGACCGTGAGGGTGCTAAAACGTTACTTCGTCAAAATACCAAACGATTAAGCCGCGTAAA
AAATGTGATGGTTGATGGCGGTTATCGAGGTGAGGCATTTGCCAATAGTGTAAAGACCCTTTTAGGCGAAACCGTTACCACAGAAGTCGCCAAAAGAGAT
GAACTGCATACGTTTAAAGTCATACCAAAGCGATGGGTTGTAGAACGTTCGTTTGCTTGGCTTGAGAAAAATAGACGTCTATGGAAAAACTGTGAGCGTC
TTTTAAATTCGAGCCTTCAATTTACCAATCTTGCTTTCATCGCTTTGTTGCTGAAAAGATTGTGAACAGGTTC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
303 bp100 aa70372+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MRKAYPSDISRKQFEHILPILESARKKTKPRTVDLYDVFCGLLYVLRTGCQWRQLPHDFPKWRTVHSYFQKWSELDEEGNSILDQALKKIGQKSAKEGQT

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
534 bp177 aa332865+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KKLVKKARKKDKRKSKTSFIIIDAQSVKNTDTAKHKGYDAGKKVSGIKRHIAVDSQGLPHAICITTANITDREGAKTLLRQNTKRLSRVKNVMVDGGYRG
EAFANSVKTLLGETVTTEVAKRDELHTFKVIPKRWVVERSFAWLEKNRRLWKNCERLLNSSLQFTNLAFIALLLKRL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
796 bp264 aa70865+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKAYPSDISRKQFEHILPILESARKKTKPRTVDLYDVFCGLLYVLRTGCQWRQLPHDFPKWRTVHSYFQKWSELDEEGNSILDQALKKLVKKARKKDKR
KSKTSFIIIDAQSVKNTDTAKHKGYDAGKKVSGIKRHIAVDSQGLPHAICITTANITDREGAKTLLRQNTKRLSRVKNVMVDGGYRGEAFANSVKTLLGE
TVTTEVAKRDELHTFKVIPKRWVVERSFAWLEKNRRLWKNCERLLNSSLQFTNLAFIALLLKRL

 

Blast result :
Comments
ISAba15 element was identified in the colistin-resistant South Korean clinical A. baumannii isolate B0707-070.
ISAba15 is 81% aa similar to ISNieu4.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Moffatt, J. H., Harper, M., Harrison, P., Hale, J. D. F., Vinogradov, E., Seemann, T., Henry, R., et al. (2010). Colistin resistance in Acinetobacter baumannii is mediated by complete loss of lipopolysaccharide production. Antimicrobial Agents and Chemotherapy, 54(12), 4971–4977.