ISAba15
- Family IS5
- Group IS1031
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
JN187417 | ND | Acinetobacter baumannii | Acinetobacter baumannii B0707-070 |
DNA section
IS Length : 873 bp
Ends
IR Length : 14/15
IRL : GAACCTGTTCACGATTAAAAAATATGCTAGGATAAGCCACATAATCGATT
IRR : GAACCTGTTCACAATCTTTTCAGCAACAAAGCGATGAAAGCAAGATTGGT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|
DNA sequence
GAACCTGTTCACGATTAAAAAATATGCTAGGATAAGCCACATAATCGATTAATAACCGAATAATTCACCATGAGAAAAGCCTATCCAAGCGACATTAGCC
GAAAACAATTTGAGCACATACTACCCATTCTAGAAAGTGCAAGGAAAAAGACCAAACCAAGAACGGTTGACCTTTATGACGTATTTTGTGGGTTATTGTA
TGTACTAAGAACAGGTTGCCAATGGCGACAGCTGCCCCATGACTTTCCCAAATGGCGCACCGTCCACTCGTACTTTCAGAAATGGAGCGAGCTTGACGAA
GAAGGCAACAGCATTCTTGACCAAGCCTTAAAAAAAATTGGTCAAAAAAGCGCGAAAGAAGGACAAACGTAAGAGCAAAACCAGTTTTATCATCATTGAT
GCTCAAAGCGTAAAAAATACCGACACGGCTAAACACAAAGGCTATGACGCAGGAAAGAAAGTATCAGGGATTAAGCGCCATATTGCGGTAGATAGCCAAG
GTTTGCCGCATGCTATCTGTATTACCACTGCCAATATCACCGACCGTGAGGGTGCTAAAACGTTACTTCGTCAAAATACCAAACGATTAAGCCGCGTAAA
AAATGTGATGGTTGATGGCGGTTATCGAGGTGAGGCATTTGCCAATAGTGTAAAGACCCTTTTAGGCGAAACCGTTACCACAGAAGTCGCCAAAAGAGAT
GAACTGCATACGTTTAAAGTCATACCAAAGCGATGGGTTGTAGAACGTTCGTTTGCTTGGCTTGAGAAAAATAGACGTCTATGGAAAAACTGTGAGCGTC
TTTTAAATTCGAGCCTTCAATTTACCAATCTTGCTTTCATCGCTTTGTTGCTGAAAAGATTGTGAACAGGTTC
GAAAACAATTTGAGCACATACTACCCATTCTAGAAAGTGCAAGGAAAAAGACCAAACCAAGAACGGTTGACCTTTATGACGTATTTTGTGGGTTATTGTA
TGTACTAAGAACAGGTTGCCAATGGCGACAGCTGCCCCATGACTTTCCCAAATGGCGCACCGTCCACTCGTACTTTCAGAAATGGAGCGAGCTTGACGAA
GAAGGCAACAGCATTCTTGACCAAGCCTTAAAAAAAATTGGTCAAAAAAGCGCGAAAGAAGGACAAACGTAAGAGCAAAACCAGTTTTATCATCATTGAT
GCTCAAAGCGTAAAAAATACCGACACGGCTAAACACAAAGGCTATGACGCAGGAAAGAAAGTATCAGGGATTAAGCGCCATATTGCGGTAGATAGCCAAG
GTTTGCCGCATGCTATCTGTATTACCACTGCCAATATCACCGACCGTGAGGGTGCTAAAACGTTACTTCGTCAAAATACCAAACGATTAAGCCGCGTAAA
AAATGTGATGGTTGATGGCGGTTATCGAGGTGAGGCATTTGCCAATAGTGTAAAGACCCTTTTAGGCGAAACCGTTACCACAGAAGTCGCCAAAAGAGAT
GAACTGCATACGTTTAAAGTCATACCAAAGCGATGGGTTGTAGAACGTTCGTTTGCTTGGCTTGAGAAAAATAGACGTCTATGGAAAAACTGTGAGCGTC
TTTTAAATTCGAGCCTTCAATTTACCAATCTTGCTTTCATCGCTTTGTTGCTGAAAAGATTGTGAACAGGTTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
303 bp | 100 aa | 70 | 372 | + | No |
Description : First part of the transposase
ORF sequence :
MRKAYPSDISRKQFEHILPILESARKKTKPRTVDLYDVFCGLLYVLRTGCQWRQLPHDFPKWRTVHSYFQKWSELDEEGNSILDQALKKIGQKSAKEGQT
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
534 bp | 177 aa | 332 | 865 | + | No |
Description : Second part of the transposase
ORF sequence :
KKLVKKARKKDKRKSKTSFIIIDAQSVKNTDTAKHKGYDAGKKVSGIKRHIAVDSQGLPHAICITTANITDREGAKTLLRQNTKRLSRVKNVMVDGGYRG
EAFANSVKTLLGETVTTEVAKRDELHTFKVIPKRWVVERSFAWLEKNRRLWKNCERLLNSSLQFTNLAFIALLLKRL
EAFANSVKTLLGETVTTEVAKRDELHTFKVIPKRWVVERSFAWLEKNRRLWKNCERLLNSSLQFTNLAFIALLLKRL
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
796 bp | 264 aa | 70 | 865 | + | Yes |
Chemistry : DDE
ORF sequence :
MRKAYPSDISRKQFEHILPILESARKKTKPRTVDLYDVFCGLLYVLRTGCQWRQLPHDFPKWRTVHSYFQKWSELDEEGNSILDQALKKLVKKARKKDKR
KSKTSFIIIDAQSVKNTDTAKHKGYDAGKKVSGIKRHIAVDSQGLPHAICITTANITDREGAKTLLRQNTKRLSRVKNVMVDGGYRGEAFANSVKTLLGE
TVTTEVAKRDELHTFKVIPKRWVVERSFAWLEKNRRLWKNCERLLNSSLQFTNLAFIALLLKRL
KSKTSFIIIDAQSVKNTDTAKHKGYDAGKKVSGIKRHIAVDSQGLPHAICITTANITDREGAKTLLRQNTKRLSRVKNVMVDGGYRGEAFANSVKTLLGE
TVTTEVAKRDELHTFKVIPKRWVVERSFAWLEKNRRLWKNCERLLNSSLQFTNLAFIALLLKRL
Blast result :
Comments
ISAba15 element was identified in the colistin-resistant South Korean clinical A. baumannii isolate B0707-070.
ISAba15 is 81% aa similar to ISNieu4.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba15 is 81% aa similar to ISNieu4.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Moffatt, J. H., Harper, M., Harrison, P., Hale, J. D. F., Vinogradov, E., Seemann, T., Henry, R., et al. (2010). Colistin resistance in Acinetobacter baumannii is mediated by complete loss of lipopolysaccharide production. Antimicrobial Agents and Chemotherapy, 54(12), 4971–4977.