ISKpn15

  • Family IS66
  • Group ISBst12
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
JQ809466 ND Klebsiella pneumonia
Klebsiella pneumonia
DNA section
IS Length : 1534 bp

Ends


IR Length : 20/27

IRL : GTAAGTATTCACCGGAACAGTATTGACACAGGAAAAATGAGACGATCGTC
IRR : GTATCTATTCACCAGGACGGGGTTGACGGCATCAGTTCTGCCGGGATGGG

Insertion site


Left flankDirect repeatRight flankDR Length
AAGCAAGGTAGTATTTGTTACAAAGAAA8

DNA sequence

GTAAGTATTCACCGGAACAGTATTGACACAGGAAAAATGAGACGATCGTCTCTTGATCCCACTTTCCTGCTTGCTTGATCCTGCTCCTTAGATACGCTCC
CCCCATGAGCAAAAACACCATGCATTCCGCGGGAATGCCCACCACTCTGGAGCAGGCCAACGAGCTGATTTATAAGCTCCTCGACCGCATCGCCGAGCTG
GAGGACCGCCTCAATCAGCACAGCGGCAACTCCTCCAAGCCGCCTTCCAGCGACGGCCCCGGTGCACCGCCGCCGGCCCGCAAGCGTCCGGCGTCCGGCA
AGCAACGGGGGGCGCAGCCCGGTCACAAGGGACATCGCCGCGAGCGGCATCCGCTTGATGCCCGCCTGACCCTGGTCCCGCATTACCCTGCCGCCGATTG
CGCTTGCTGTGGCGGGGACATGGTGGCCCATGCCAAACCCTATCAGGTTCATCAGGTGTTCGAGCTGCCCGAGGTGAGCTATCTCGTCACCGAACACCAA
CTGTATCGTGCGACCTGCTCACGGTGTATCGGCACGACCCAGGCCGCACTGCCAGACAACGTCAGCAGCAGCCAGATGGGGGCCAATCTGCTCAGCTACA
TCGCGCTGCAAAGCGGCCAGTTTCACCAGAGCGTCAGCCAGATACAGCAGCAGCTCAAACAGCATTTCGGCCTGAGTTTTAGCCGGGGCGCCATCAGTGA
GGCGCAGGGGCGGGTGAGTGCCATGCTCACCCCGACCTACCAGGCCATCAAGCAGCAGGTGCAGACGGCCACCCACGTTCATGCCGATGAGACCCGGCAT
CAGCGGGGTGGAGAGCGCCGCTGGATGTGGCTGGCGCTGAGCAAGATAGCCGTCTGCTTCATGACGGCCTATGGCCGTGGTCAGGAGGCGGCCAAGCGGC
TGCTGGGCGCCGAGTTTGACGGGGTGCTGGTGACCGACCAGTACGCCGGCTACCGGTTTATCGACGCCGACCAGCGGCAACTGTGCTGGGCCCACGTGGC
CCGCAATCTCGTGGCCATCGCCGAGAGTAGCGAGCAGTGCAACCAGCCCATCGGTGCCCGGTTGGTCTTGCTGGCGGATGCGGTGTTTCGTACCCGGCAC
CGTTGGGAAAGCGGTGCGTTGAGCAAGCAGAAATACCTGCGCAGGCTCGCGCGTTACCGGCAAAGCTGGCGAAGCCAGTTGGAGCGCGGCGCCCTGTTGT
GCAGCAAACGCTACCGTGGACGATGCCAGTTGCTGCTGCGGGATGACGAGATGCTGTGGCGGTTTATGACTGACGACGAAATAGCGCTGACCAACAACGA
GGCGGAGCGGGCGCTGCGTGGCTATGTGCTCTGGCGCAAGGGTAGCTACGGCGTGTGGTCTCACCGGGGCGAGCTGTTTCGCCAGCGCATCCTGTCGTTG
GTGGAGACCGGCAAGCGATTGGGCCGCTGCCCACAGGAGTGGTTGAGGGCCGTGGTCAGGGCGTGCATCGAGAAGACAGATTATCCCATCCCGGCAGAAC
TGATGCCGTCAACCCCGTCCTGGTGAATAGATAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1422 bp473 aa1051526+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSKNTMHSAGMPTTLEQANELIYKLLDRIAELEDRLNQHSGNSSKPPSSDGPGAPPPARKRPASGKQRGAQPGHKGHRRERHPLDARLTLVPHYPAADCA
CCGGDMVAHAKPYQVHQVFELPEVSYLVTEHQLYRATCSRCIGTTQAALPDNVSSSQMGANLLSYIALQSGQFHQSVSQIQQQLKQHFGLSFSRGAISEA
QGRVSAMLTPTYQAIKQQVQTATHVHADETRHQRGGERRWMWLALSKIAVCFMTAYGRGQEAAKRLLGAEFDGVLVTDQYAGYRFIDADQRQLCWAHVAR
NLVAIAESSEQCNQPIGARLVLLADAVFRTRHRWESGALSKQKYLRRLARYRQSWRSQLERGALLCSKRYRGRCQLLLRDDEMLWRFMTDDEIALTNNEA
ERALRGYVLWRKGSYGVWSHRGELFRQRILSLVETGKRLGRCPQEWLRAVVRACIEKTDYPIPAELMPSTPSW

 

Blast result :
Comments
ISKpn15 is 89% aa similar to ISUnCu16.
References
1] Poirel L. (2012) Direct submission