ISPeth4

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009454 ND Pelotomaculum thermopropionicum
Pelotomaculum thermopropionicum SI
DNA section
IS Length : 1488 bp

Ends


IR Length : 25/32

IRL : GAGAACGTAAAAAGTTTTGTGTAAATGGTTATAACTAACAATAAAAGGAG
IRR : GGGAGTGTAAATTATTTTGTGTAAATGGGTATTCCCAGAAAAAATTGGTT

Insertion site


Left flankDirect repeatRight flankDR Length
CTTTCACTCGCCGGATTCCCCCCAGATG8
GGTTAGAAAAATTTATTAGCTTGTTTAT8
GTTTGTTTTGGGGAAACTCCTTGTCTCC8

DNA sequence

GAGAACGTAAAAAGTTTTGTGTAAATGGTTATAACTAACAATAAAAGGAGCTGACCATTTATGCAAAACACTATGGACAAAACCATCAAAGAATTAGCCA
GAGAATGTGAAACCGTAGAAGATGTACACAACATGCTTAAGAACCTCTTTAAAGACACTTTGCAGCAAATATTTGAGGCCGAGATGGATGAACACTTAGG
GTATAAAAAGCATAGCCCGGAGGGTAATAATACAGGTAACAGCCGCAATGGTTACAGCAAGAAAACCATCCAGACCAAGCTGGGCAAAACCGAAATAGAA
ATACCCCGTGATCGCAATGGAGAATTTGAACCCAGGATTATCAAAAAGTACCAGACAACCTCAAATGACCTGGAAGACCAGATTATCGCCATGTACGCCA
AGGGTATGACCACCCGGGATATTGAGGATCATATGCGGGATATCTATGGTATCGACGTATCCCCGGTAATGGTCAGCAAGGTGACGGACAAAATAATGCC
TATGATCGCCGAGTGGCAGTCAAGACCCTTAGAACGCGTATACCCTATCATTTACCTGGATGCCATTTTCTTTAAAGTGCGCAAGGACAACCGTATCATA
AACAAGGCTGCCTACAGCGTACTGGGCATCAACATGGCCGGCCAGAAGGACATACTGGGCATCTGGATTGGGGAAAATGAAAGTGCCAGCTTCTGGCTTG
GAGTGTGCAACGACCTGAAAAACAGAGGCGTGCAGGACATCCTGATTGCCTGTAAAGACGGGCTTTCCGGGTTTTCCGAGGCTATCAATACCGTGTTTCC
CCGGACAGAAATCCAGCTATGCATAATACATCAAATCCGTAATTCCCTGAAATACGTGCCTTGGAAGGAACAAAAGGAATTAATGGCCGACCTCAAGAAA
GTATACCAGGCCCTTACCATGGAGGAAGCGGAACTCGCCTTTAAAATGTTCAAGGAGAAGTGGGGTAAAAAACACCCGGTTATTATCCGCTCCTGGGAGA
AAAACTGGCTGGAACTGACGGCTTACTTCAAGTACCCATACGAGATTCGCAAACTCATTTACACTACCAATATCATCGAAGGATACCACCGGCAGCTACG
CAAGGTAACCAAGACAAAAACCGCGTATCCGACGGATGACGCCTTAAAAAAGATTATCTACCTGGCTACTGTTGAGGCATCCAAAAAGTGGACTGTACCG
ATCAGGGAGTGGAAAAGTTGTATCTCACAGTTTGCCATCCACTTCAGCGATAGGCTGGAACCGGAACTGATGGTTGGATAATGAGTTTACCGCTTTTGTT
CTCCCAAGGATAGCAGGAAAATGATAGCAGGGCAGCCTCTAGGCCACCCCTGCTGTCCTCGGCAGAACCCTGCCAGGCGCTCGGGTTGCTCTCCAGCAGT
GCCCTACCCTCCTGGCAGGTAAGAGCCATTTATAGCTTAACCAATTTTTTCTGGGAATACCCATTTACACAAAATAATTTACACTCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1221 bp406 aa611281+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQNTMDKTIKELARECETVEDVHNMLKNLFKDTLQQIFEAEMDEHLGYKKHSPEGNNTGNSRNGYSKKTIQTKLGKTEIEIPRDRNGEFEPRIIKKYQTT
SNDLEDQIIAMYAKGMTTRDIEDHMRDIYGIDVSPVMVSKVTDKIMPMIAEWQSRPLERVYPIIYLDAIFFKVRKDNRIINKAAYSVLGINMAGQKDILG
IWIGENESASFWLGVCNDLKNRGVQDILIACKDGLSGFSEAINTVFPRTEIQLCIIHQIRNSLKYVPWKEQKELMADLKKVYQALTMEEAELAFKMFKEK
WGKKHPVIIRSWEKNWLELTAYFKYPYEIRKLIYTTNIIEGYHRQLRKVTKTKTAYPTDDALKKIIYLATVEASKKWTVPIREWKSCISQFAIHFSDRLE
PELMVG

 

Blast result :
Comments
ISPeth4 is 73% aa similar to ISCbo4.
References
1] Romain Guérillot and Philippe Glaser (2013) Direct submission.
2] Kosaka,T., Kato,S., Shimoyama,T., Ishii,S., Abe,T. and Watanabe,K. ( 2008) Genome Res. 18 (3), 442-448.
3] Kosaka,T., Uchiyama,T., Ishii,S., Enoki,M., Imachi,H., Kamagata,Y., Ohashi,A., Harada,H., Ikenaga,H. and Watanabe,K. (2006) J. Bacteriol. 188 (1), 202-210.