ISKpn18
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP003200 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae subsp. pneumoniae HS11286 plasmid pKPHS3 Klebsiella pneumoniae subsp. pneumoniae HS11286 |
DNA section
IS Length : 1303 bp
Ends
IR Length : 26/33
IRL : TGTTGTGGTCAAATTTAAATGGACACTTTGATCGGGTGTTTTCCCCCCGA
IRR : TGTTGTGGTCAAGTTTTTTTGGACACGCCGATCAGATGGTAGGGTTATGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCCACATGG | TGG | CTTCAAAGGC | 3 |
| GCTTAACGCT | GAC | AAACAGCCGC | 3 |
| TCATCGACAC | CTCTGTAACC | 0 |
DNA sequence
TGTTGTGGTCAAATTTAAATGGACACTTTGATCGGGTGTTTTCCCCCCGACAAGGAACATGAAGAGATGACCGACAAGACAACCGACACAACCAAGAATC
GCCGAACCTTTGATCCAGCGTTCAAGTTACAGGTGGCGCGCATGGTGCGCGAGCAAGGCGTCAGCGTCAGCCAAGTCTGCCGCGAAATGAAGCTGACCGA
AAGTGTGGTCCGACGCTGGGTACACCAACTGGATGACGAGTCAGCCGGCAGGCCGGGGATTGGTAAACCGTTAACGGCTGAGCAGCAACGTATTCGAGAA
CTGGAAGCCGAAGTCAGGCGACTAAAATCAGACAATGAATTATTAAAAAAAGCTTCGGCCTTCTTCGCACGGGAAATAAAGTGATCCACGATGTTGTCAC
TGAGTTAGCGAAGCAGGCCCCGATCAAGCAGGTCTGTCAGGCGTTGGACATCAGTCGCTCTGGTTACTACGCAGCGCGTCGCAGAGTCAATACACCCAAA
CCTATTTGCCAGGACAGCGTGCATGCTCAGGCGGTTTTTCTTGCCAGTGGTCGCACCTATGGCAGTCGTCGTTTGAGTGCAGCCCTGCGTGCCCAGGGCC
ATGATGTTGGACGTCATCGCTGTCGTACCTTGATGAAAGCCTGTGGACTCAAGGCCTGTTGGCGTCGCAAGTTCGCTCATACCACCGATAGCAAACACCA
TTTGCCGGTGGCAGAGAACGTGCTCAACCGTCAGTTTGAGCCTGAAGCACCCAATACGGCCTGGGTGTGCGATGTGACGTACATTCGGACCGACAGCGGT
TGGCTATATCTGGCCGCTGTGCTGGATCTGTATTCACGTCGCATCGTGGGTTGGTCCATGGCACCGACCATGCCTGCACAGCTGGTGTGCGATGCGTTGA
GCATGGCTATTTCGGTTCGCCAACCGGCACCGGGTTTATTGGTTCATTCGGACCGGGGCAGTCAATATGCCAGTGCTGAACACCGGCAATTACTTGAAAA
CCATGGTCTGGTACTCAGCATGAGCCGCAAGGGTAATTGCTGGGACAATGCCGTGATGGAGCGGTTCTTTCTGAATTTGAAAATGGAGCGTGTCTGGCAG
ACTCGGTATGCCAACCATACAGAAGCCAAACAAGACATCACCGACTACATCGTTAACTTTTATAACAGTACCCGATTGCATTCAAAACTTGGCTACCGAT
CACCCGCACAGTACGAAACGCTGGCCAGCCCTGCCGCCCAGTTGCCCGCCTGTGCATAACCCTACCATCTGATCGGCGTGTCCAAAAAAACTTGACCACA
ACA
GCCGAACCTTTGATCCAGCGTTCAAGTTACAGGTGGCGCGCATGGTGCGCGAGCAAGGCGTCAGCGTCAGCCAAGTCTGCCGCGAAATGAAGCTGACCGA
AAGTGTGGTCCGACGCTGGGTACACCAACTGGATGACGAGTCAGCCGGCAGGCCGGGGATTGGTAAACCGTTAACGGCTGAGCAGCAACGTATTCGAGAA
CTGGAAGCCGAAGTCAGGCGACTAAAATCAGACAATGAATTATTAAAAAAAGCTTCGGCCTTCTTCGCACGGGAAATAAAGTGATCCACGATGTTGTCAC
TGAGTTAGCGAAGCAGGCCCCGATCAAGCAGGTCTGTCAGGCGTTGGACATCAGTCGCTCTGGTTACTACGCAGCGCGTCGCAGAGTCAATACACCCAAA
CCTATTTGCCAGGACAGCGTGCATGCTCAGGCGGTTTTTCTTGCCAGTGGTCGCACCTATGGCAGTCGTCGTTTGAGTGCAGCCCTGCGTGCCCAGGGCC
ATGATGTTGGACGTCATCGCTGTCGTACCTTGATGAAAGCCTGTGGACTCAAGGCCTGTTGGCGTCGCAAGTTCGCTCATACCACCGATAGCAAACACCA
TTTGCCGGTGGCAGAGAACGTGCTCAACCGTCAGTTTGAGCCTGAAGCACCCAATACGGCCTGGGTGTGCGATGTGACGTACATTCGGACCGACAGCGGT
TGGCTATATCTGGCCGCTGTGCTGGATCTGTATTCACGTCGCATCGTGGGTTGGTCCATGGCACCGACCATGCCTGCACAGCTGGTGTGCGATGCGTTGA
GCATGGCTATTTCGGTTCGCCAACCGGCACCGGGTTTATTGGTTCATTCGGACCGGGGCAGTCAATATGCCAGTGCTGAACACCGGCAATTACTTGAAAA
CCATGGTCTGGTACTCAGCATGAGCCGCAAGGGTAATTGCTGGGACAATGCCGTGATGGAGCGGTTCTTTCTGAATTTGAAAATGGAGCGTGTCTGGCAG
ACTCGGTATGCCAACCATACAGAAGCCAAACAAGACATCACCGACTACATCGTTAACTTTTATAACAGTACCCGATTGCATTCAAAACTTGGCTACCGAT
CACCCGCACAGTACGAAACGCTGGCCAGCCCTGCCGCCCAGTTGCCCGCCTGTGCATAACCCTACCATCTGATCGGCGTGTCCAAAAAAACTTGACCACA
ACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 67 | 384 | + | No |
Description : First part of the transposase
ORF sequence :
MTDKTTDTTKNRRTFDPAFKLQVARMVREQGVSVSQVCREMKLTESVVRRWVHQLDDESAGRPGIGKPLTAEQQRIRELEAEVRRLKSDNELLKKASAFF
AREIK
AREIK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 921 bp | 306 aa | 339 | 1259 | + | No |
Description : Second part of the transposase
ORF sequence :
IIKKSFGLLRTGNKVIHDVVTELAKQAPIKQVCQALDISRSGYYAARRRVNTPKPICQDSVHAQAVFLASGRTYGSRRLSAALRAQGHDVGRHRCRTLMK
ACGLKACWRRKFAHTTDSKHHLPVAENVLNRQFEPEAPNTAWVCDVTYIRTDSGWLYLAAVLDLYSRRIVGWSMAPTMPAQLVCDALSMAISVRQPAPGL
LVHSDRGSQYASAEHRQLLENHGLVLSMSRKGNCWDNAVMERFFLNLKMERVWQTRYANHTEAKQDITDYIVNFYNSTRLHSKLGYRSPAQYETLASPAA
QLPACA
ACGLKACWRRKFAHTTDSKHHLPVAENVLNRQFEPEAPNTAWVCDVTYIRTDSGWLYLAAVLDLYSRRIVGWSMAPTMPAQLVCDALSMAISVRQPAPGL
LVHSDRGSQYASAEHRQLLENHGLVLSMSRKGNCWDNAVMERFFLNLKMERVWQTRYANHTEAKQDITDYIVNFYNSTRLHSKLGYRSPAQYETLASPAA
QLPACA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1193 bp | 397 aa | 67 | 1259 | + | Yes |
Chemistry : DDE
ORF sequence :
MTDKTTDTTKNRRTFDPAFKLQVARMVREQGVSVSQVCREMKLTESVVRRWVHQLDDESAGRPGIGKPLTAEQQRIRELEAEVRRLKSDNELLKKSFGLL
RTGNKVIHDVVTELAKQAPIKQVCQALDISRSGYYAARRRVNTPKPICQDSVHAQAVFLASGRTYGSRRLSAALRAQGHDVGRHRCRTLMKACGLKACWR
RKFAHTTDSKHHLPVAENVLNRQFEPEAPNTAWVCDVTYIRTDSGWLYLAAVLDLYSRRIVGWSMAPTMPAQLVCDALSMAISVRQPAPGLLVHSDRGSQ
YASAEHRQLLENHGLVLSMSRKGNCWDNAVMERFFLNLKMERVWQTRYANHTEAKQDITDYIVNFYNSTRLHSKLGYRSPAQYETLASPAAQLPACA
RTGNKVIHDVVTELAKQAPIKQVCQALDISRSGYYAARRRVNTPKPICQDSVHAQAVFLASGRTYGSRRLSAALRAQGHDVGRHRCRTLMKACGLKACWR
RKFAHTTDSKHHLPVAENVLNRQFEPEAPNTAWVCDVTYIRTDSGWLYLAAVLDLYSRRIVGWSMAPTMPAQLVCDALSMAISVRQPAPGLLVHSDRGSQ
YASAEHRQLLENHGLVLSMSRKGNCWDNAVMERFFLNLKMERVWQTRYANHTEAKQDITDYIVNFYNSTRLHSKLGYRSPAQYETLASPAAQLPACA
Blast result :
Comments
ISKpn18 is 63% aa similar to ISPosp5.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] A. Garcia-Fernandez (2013) Direct submission