ISAnhy1
- Family ISLre2
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_ACJX02000009.1 | ND | Anaerobaculum hydrogeniformans | Anaerobaculum hydrogeniformans ATCC BAA-1850 |
DNA section
IS Length : 1571 bp
Ends
IR Length : 22/28
IRL : GATCGCGTAAAATATTTGTAGGAGATTTTCAGAGGTAAAAAGAAAGGGAC
IRR : GACCGCGTCAAGTTTTTGTAGGATTTTTAGGAACCACTCCCCATGTGTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTGCGGTCAT | GGAAAAGACT | 0 | |
| GGGGGTATAG | GAGAACAAA | CTTCTGCTTG | 9 |
DNA sequence
GATCGCGTAAAATATTTGTAGGAGATTTTCAGAGGTAAAAAGAAAGGGACCCCTCCTTTTGGTAGAATCTTTGTAGCACACAAAAACTCATAAAGGAGGG
TCCCAACTTGAACAAGGATATTTTACTACAAATAGCAATAAACTTCATCAAGGAGTTGCTTGAATTCTTCGGTGATTCCGAGGTCAGGACATTAGCGGAA
ATTGAAGACGAAATCTCTCGGATCATGAAGGCCTTCATCCGAGAACTGATAAAGGCTTACTTCGAGCTTGCAGATGAGGCCATATTAAAGGACAAAACAT
CTCGAAAGGAAAGAGGGTTAGTCGTAGAAAGGCGTGAGGACAAAAGAAGCGTTTATACCATCTTTGGGGATATATCTTTTAGCAGGACCTATTATTACGA
CAAAAAGCGAAACGCGTACATCTACCCCCTGGACAAAGCATTAGGGTTGGATAAATACGAAAGGCTGACTGAAGCGGTGGCCATAAAGCTGGTAGAAACG
GCAGGACAGGTATCTTACGCCAAAAGCTCGATGAACGTAACTAATGGCGAGTTATCCAGGCAGACCGTCAAAAACAAGATACACAACCTAAACCTTGAGG
CCTTGAAGACAAAAGTTCCTGAGAAAAGAAGCGTAAATGTATTACATATAGATGCGGATGAGGACCACGCATCGTTACAAGACGGCAGAAGCACGAATCT
TCCTTTGATCTGCATATACGAGGGCACCTTTAAAGAGGGATCCAAAAACAGATGTATAAACCCCATATACATGAGCGGCTATGGAAAGGGCAGCGATGAG
TTTTGGCTTGAGGTAACAGATAAGATATACGAGCTTTATGACCCGGAGGACATAAAGGGCATATACATCCATGGAGATGGTGCAAATTGGATAAAGGAAG
GGATAAACTGGCTGCCCGAGTCAAAGCTGGTGCTGGATAAATTCCACCTAAATAAAGCAATCCTGGAAGCCACGGCAAGGCAGCCCGAAAAAAGACGTGA
TATATACAGGGCCATAAATACAAGTGACTTGAACTTGTTTAAGAAAATAACCACCGAGATGGCTAAATGTGCTTCAAGCGAGAGGGAAAGAAAGATGATC
GCAGATTTTAGGAGATATATAACCAACAACTGGGGCTCCATTACCATTCGAAACGAAGAGGACTGCGGGAGCTCAAGTCCCGAGGGTCATATTAGCCACG
TACTTTCCTCTAGGTTAAGTTCTAGGCCTATAGCATGGAGCCGCAAGGGCTTAAAGGCCATGTCTTCTTTGAGGGCTTACATCTGTAGCGGTGGCAAGGT
GACGTCAGAACAGATAAAGAAAAAAGACCAAAAAGGTGAGAATGCGGACAAAAGACACAAATTCACCTTGAACCTGGAGGAGATCTTAGGTCCCGTTATA
AGTGAGCTTGGATGCATAACGGTACTGAAAACGGGAAAGGTAACGCCCTTATACACGACCTTAAGGGGCATATGTCATGGCGGGTTTGATTTTTACCTAA
CCTAAAAGACGTTGCCCTTTAGGACACATGGGGAGTGGTTCCTAAAAATCCTACAAAAACTTGACGCGGTC
TCCCAACTTGAACAAGGATATTTTACTACAAATAGCAATAAACTTCATCAAGGAGTTGCTTGAATTCTTCGGTGATTCCGAGGTCAGGACATTAGCGGAA
ATTGAAGACGAAATCTCTCGGATCATGAAGGCCTTCATCCGAGAACTGATAAAGGCTTACTTCGAGCTTGCAGATGAGGCCATATTAAAGGACAAAACAT
CTCGAAAGGAAAGAGGGTTAGTCGTAGAAAGGCGTGAGGACAAAAGAAGCGTTTATACCATCTTTGGGGATATATCTTTTAGCAGGACCTATTATTACGA
CAAAAAGCGAAACGCGTACATCTACCCCCTGGACAAAGCATTAGGGTTGGATAAATACGAAAGGCTGACTGAAGCGGTGGCCATAAAGCTGGTAGAAACG
GCAGGACAGGTATCTTACGCCAAAAGCTCGATGAACGTAACTAATGGCGAGTTATCCAGGCAGACCGTCAAAAACAAGATACACAACCTAAACCTTGAGG
CCTTGAAGACAAAAGTTCCTGAGAAAAGAAGCGTAAATGTATTACATATAGATGCGGATGAGGACCACGCATCGTTACAAGACGGCAGAAGCACGAATCT
TCCTTTGATCTGCATATACGAGGGCACCTTTAAAGAGGGATCCAAAAACAGATGTATAAACCCCATATACATGAGCGGCTATGGAAAGGGCAGCGATGAG
TTTTGGCTTGAGGTAACAGATAAGATATACGAGCTTTATGACCCGGAGGACATAAAGGGCATATACATCCATGGAGATGGTGCAAATTGGATAAAGGAAG
GGATAAACTGGCTGCCCGAGTCAAAGCTGGTGCTGGATAAATTCCACCTAAATAAAGCAATCCTGGAAGCCACGGCAAGGCAGCCCGAAAAAAGACGTGA
TATATACAGGGCCATAAATACAAGTGACTTGAACTTGTTTAAGAAAATAACCACCGAGATGGCTAAATGTGCTTCAAGCGAGAGGGAAAGAAAGATGATC
GCAGATTTTAGGAGATATATAACCAACAACTGGGGCTCCATTACCATTCGAAACGAAGAGGACTGCGGGAGCTCAAGTCCCGAGGGTCATATTAGCCACG
TACTTTCCTCTAGGTTAAGTTCTAGGCCTATAGCATGGAGCCGCAAGGGCTTAAAGGCCATGTCTTCTTTGAGGGCTTACATCTGTAGCGGTGGCAAGGT
GACGTCAGAACAGATAAAGAAAAAAGACCAAAAAGGTGAGAATGCGGACAAAAGACACAAATTCACCTTGAACCTGGAGGAGATCTTAGGTCCCGTTATA
AGTGAGCTTGGATGCATAACGGTACTGAAAACGGGAAAGGTAACGCCCTTATACACGACCTTAAGGGGCATATGTCATGGCGGGTTTGATTTTTACCTAA
CCTAAAAGACGTTGCCCTTTAGGACACATGGGGAGTGGTTCCTAAAAATCCTACAAAAACTTGACGCGGTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1404 bp | 465 aa | 102 | 1505 | + | No |
Chemistry : DDE
ORF sequence :
MNKDILLQIAINFIKELLEFFGDSEVRTLAEIEDEISRIMKAFIRELIKAYFELADEAILKDKTSRKERGLVVERREDKRSVYTIFGDISFSRTYYYDKK
RNAYIYPLDKALGLDKYERLTEAVAIKLVETAGQVSYAKSSMNVTNGELSRQTVKNKIHNLNLEALKTKVPEKRSVNVLHIDADEDHASLQDGRSTNLPL
ICIYEGTFKEGSKNRCINPIYMSGYGKGSDEFWLEVTDKIYELYDPEDIKGIYIHGDGANWIKEGINWLPESKLVLDKFHLNKAILEATARQPEKRRDIY
RAINTSDLNLFKKITTEMAKCASSERERKMIADFRRYITNNWGSITIRNEEDCGSSSPEGHISHVLSSRLSSRPIAWSRKGLKAMSSLRAYICSGGKVTS
EQIKKKDQKGENADKRHKFTLNLEEILGPVISELGCITVLKTGKVTPLYTTLRGICHGGFDFYLT
RNAYIYPLDKALGLDKYERLTEAVAIKLVETAGQVSYAKSSMNVTNGELSRQTVKNKIHNLNLEALKTKVPEKRSVNVLHIDADEDHASLQDGRSTNLPL
ICIYEGTFKEGSKNRCINPIYMSGYGKGSDEFWLEVTDKIYELYDPEDIKGIYIHGDGANWIKEGINWLPESKLVLDKFHLNKAILEATARQPEKRRDIY
RAINTSDLNLFKKITTEMAKCASSERERKMIADFRRYITNNWGSITIRNEEDCGSSSPEGHISHVLSSRLSSRPIAWSRKGLKAMSSLRAYICSGGKVTS
EQIKKKDQKGENADKRHKFTLNLEEILGPVISELGCITVLKTGKVTPLYTTLRGICHGGFDFYLT
Blast result :
Comments
ISAnhy1 is 59% aa similar to ISTte3.
ISs of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
ISs of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2012) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted);
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted);
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).