ISLcr2

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_GL531739.1 ND Lactobacillus crispatus
Lactobacillus crispatus CTV-05
DNA section
IS Length : 1599 bp

Ends


IR Length : 18/24

IRL : CTAAGTGTATAATTTTTTACGGTAAGTTGAATAAATAATTAAAAAAGCCC
IRR : GTAAGTGTCAATGTTTTTGCGGTAGCCTTTAAACCATTAGTCTAACCTTA

Insertion site


Left flankDirect repeatRight flankDR Length
AAGGGCGCTACCCCTTCAGCGTGCAGTTT9
ATTCAAGTCATTTATAACATAAAATATGT9
CGTAAAGCTAAGACTATCACTATTGAAGT9

DNA sequence

CTAAGTGTATAATTTTTTACGGTAAGTTGAATAAATAATTAAAAAAGCCCAAGTCCTTTGAATTTGGACTTGAGCTCAAACAAAAAGACCAGTAGTCTGA
TGTTAAGAACAAAAAACACATTAGAAGCTGGTCTTTTATGAATTCAATTGTAACTGAAATCGCAAATATTATAAAGTCTGAAGATAATTATATTAAGCGT
GAAAGAAAAATAATCTGTTTCTTCTTAAACCTGATTAAGGAAATCATGGCCTTGGCTTTAGCTAAAGTTGATGATGAAATGATTACCAAGGTTAAAGCAC
AGGGCTATCAGATTGATAAGAAGAATGAGCGCTCAATCAATATGGCTTTTGGCGAAGTTAGGTATGTCAGAAGAAGATATGTCTGCCCAGGCAAACAAGC
AAGGTATCCCTTGGATGAATTAATGGGCTTTGACAAATACAAGCGCTACAGTATTTTAGCAGTCAAAAACATCTTAGAAGTAAGCAGCGTTGCCACTTAT
CGCAATACTGCTCTAGCAGTCAATTGCCTGTCTGGCTTTAATATTAGCCATATGCAGGTGGGCAATTTGGTAAAAATGGCCGGGAAAAATATTAAAGCGG
GCCAAGAAGCAGATTCAAGATATGATGCCGCAGGCACTAAGAAAAAGGTCCCAGTTTTGTATCTTGAAGGCGATGGCGTGGTGATCAAAGGCACTAAAAG
CCGGTTGGAATTTCATCGCTATCAGGTCTGTGAAGGCATTGTCAATATAAGCAAGAAACGCAGAAAGCGAATCAATGCCAAGGAATTTGTTTCTTTAAGC
CGACTTGATGCGCTGAACGAAGCCAAGGAGTACCTGGCCAATACTTATGATCTTTCTGATACTGTAATTCTTAGCAACGCCGATGGCGGTGCTGGATATG
CCAAAACAGACTTCGATGACATTGTAGGGCTATGCAAGCGTCATGAGCATTTCTTGGATGTCTTTCACCTAAATAAGAAAATCAAAGAAAGGCTATATTT
TGCGCCTGACCTGCAAGGCAAGCTGATTTCTGCTTTGGAATACAAATACAGCTATGACAAAGTTAAAACTATCCTAACCACTGCAGAAAGCCGACTAATT
GATGAATTGAATACCGAGCTCAATCATGATTATCTGGGGCGCCTAAGGAATTATATTGATCGTCATTGGGACGATATTAAGCCGTTTAAGAAACGAAAAC
TGACTGTGACTAAAGCCATTGGCTGCTGCGAGAGCAACCACCGCAAGTATACCTACCGTGTTAAGGGGCAAGGCAAGAAATGGTCAAAATCTGGAGCAGA
AGGCATGCTTAGGATCTTAAGCTGCATCAAGAATCGAGAGCTGGAATTTTGGCTTAGCAGCGAGTTTGAAGATTCAGAAATAACTGTATTGACTGAACAG
GAATTAAAAGCAGCGGCCCGTGACAGCTTAAAGAAGCGTCATGAAGTTCATTTAGGCATCCAGCATGGCGCATTAACCGCACAGGTAGCTGGCAATAGTT
ATTTAAGCAAGCTAAGCAGAAATTTAAGTCATATAAATATTTAAGAAATTAAGGTTAGACTAATGGTTTAAAGGCTACCGCAAAAACATTGACACTTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1407 bp468 aa1381544+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNSIVTEIANIIKSEDNYIKRERKIICFFLNLIKEIMALALAKVDDEMITKVKAQGYQIDKKNERSINMAFGEVRYVRRRYVCPGKQARYPLDELMGFDK
YKRYSILAVKNILEVSSVATYRNTALAVNCLSGFNISHMQVGNLVKMAGKNIKAGQEADSRYDAAGTKKKVPVLYLEGDGVVIKGTKSRLEFHRYQVCEG
IVNISKKRRKRINAKEFVSLSRLDALNEAKEYLANTYDLSDTVILSNADGGAGYAKTDFDDIVGLCKRHEHFLDVFHLNKKIKERLYFAPDLQGKLISAL
EYKYSYDKVKTILTTAESRLIDELNTELNHDYLGRLRNYIDRHWDDIKPFKKRKLTVTKAIGCCESNHRKYTYRVKGQGKKWSKSGAEGMLRILSCIKNR
ELEFWLSSEFEDSEITVLTEQELKAAARDSLKKRHEVHLGIQHGALTAQVAGNSYLSKLSRNLSHINI

 

Blast result :
Comments
ISLcr2 is 80% aa similar to ISLcr1.
ISs of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2012) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted);
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).