ISLhe11

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_017467 ND Lactobacillus helveticus
Lactobacillus kefiranofaciens ZW3
Lactobacillus helveticus H10
Lactobacillus kefiranofaciens ZW3 plasmid pWW1
DNA section
IS Length : 1597 bp

Ends


IR Length : 18/24

IRL : CTAAGTGTAAAATAGTTTGCGGTAACGTTTAAATATAAATTAAAAGGAGT
IRR : GTAAGTGTCAATATTTTTGCGGTAGTCCAAAAACCATTAGGCTGACCTTA

Insertion site


Left flankDirect repeatRight flankDR Length
TAGCCCTGTCTTAATTTGTTACCACATTG9
AAATAAAATGCTAAATTCTT0
AACCAACCGTAAACTGCTTT0
AACCAACCGTGCAAGGTAGT0

DNA sequence

CTAAGTGTAAAATAGTTTGCGGTAACGTTTAAATATAAATTAAAAGGAGTCAAATCCATTTCAGGACTTGACTCCAAAACAAAAAGACCAGTAGTCTGAT
GCTAAGCAAAAAAACACATTAGAAGCTGGTCTTTTATGGAATTAAGTGTAACTGAAATCGTCAAAATTATAAAGTCTGAAACTAATATTATTAAGCGTGA
AAAAGCTATAGCCTTTTTCTTTCTCAATTTGATCAGAGAATTGATGTCCTTAGCCTTGGAACGAGCAGATCAAGAGCTAAGTGAATCAATGAAGAATCAG
GGCTACCAAATTGAAAAGAAGAATCAACGTTCAATCAATATGGCTTTTGGCGAAGCGACTTATGTCAGAAGAAGGTACGTTAAAGCTGGGCAAGAAAGTC
GCTATCCTTTAGATAAATTCATGGGCTTTGATAAGTACAAGCATTATAGCGTCTTGGCAGTAAGAAATATCCTGGCAGTAAGCAGCGTCGCTGCTTATCG
CAATACTGCCTTAGCTGTTAATTGTCTTTCGGGTTTTAATATTAGTCACGCTCAGATTGGCAATTTAGTTAAGACTGCAGGTCAAAAGATCAAAGAGCAA
CAAGAAGCTGATAGTCGTTATGATGCTCAAACTGCAAAGAAGCAAGTGCCAGTTCTTTGTCTTGAAGGCGATGGCGTCATGATTAAAGGCACTCAAGGAC
GATTTGAGTTTCATCGCTATCAAGTTTGTGAAGGATTACGTAATGTGACCTGCAAACGTAGAGAGCGGACTAATGCCAAAGAATTTGTGTCATTAAGTCG
TCTAGACGCTTTGAATGAAGCTAAGGAATATATAGCTAACACTTATGATTTAGCTAATACCTTGATCATCAGTAATGCCGATGGTGGTGCTGGTTACGCG
AAAAAGGACTTTGATGAAATTGTCGGTCGTTGCGCTAAACATGAGCACTTTTTGGATGTCTTCCATTTGAACAAAAAGATTAAAGACCGACTTTGTTTCG
CTCCAGAACTGCAAGGCAAATTGATTTATGCCTTAGAGTTCAAATATGACCGCGATTTAGTGAATATAATTCTTGATACTGCCGAAAGCAAACTAATTGA
TGAGTTAGATACACCACAAAATCACGAGCATTTAGCACGGCTTCGCAGCTATATTAATCGACGCTGGGACAACATTAAACCGTTTAAAATGCGTAATTTG
AAGGTTACTAAGGCAATTGGCAGCTGTGAAAGCAATCGCCGTAAGTACACTTATCGTGTTAAAGGCCAAGGCAAATACTGGTCTGAAGCTGGAGCAGAAG
GCATGCTTCGGATTTTGGCCTGTATCAAGAATCGTGACTTAGAACAATGGCTAAATAGTGATTTTGAAGCAGGCAAATTAATCAGCTTAGATCACAAGAA
GCTGCTGGCAGCGGCCAGAGACAGCTTGAAGCGTCGCCATGAAATTCATGTTGGCATTCAACACGGAGCACTAACAGCAGAGGCTGTCGGGGGAAGCTAT
TTAGCTAAATTAGATCGAATGTTAAACCACATAAAATATTAAGAAATTAAGGTCAGCCTAATGGTTTTTGGACTACCGCAAAAATATTGACACTTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1407 bp468 aa1361542+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MELSVTEIVKIIKSETNIIKREKAIAFFFLNLIRELMSLALERADQELSESMKNQGYQIEKKNQRSINMAFGEATYVRRRYVKAGQESRYPLDKFMGFDK
YKHYSVLAVRNILAVSSVAAYRNTALAVNCLSGFNISHAQIGNLVKTAGQKIKEQQEADSRYDAQTAKKQVPVLCLEGDGVMIKGTQGRFEFHRYQVCEG
LRNVTCKRRERTNAKEFVSLSRLDALNEAKEYIANTYDLANTLIISNADGGAGYAKKDFDEIVGRCAKHEHFLDVFHLNKKIKDRLCFAPELQGKLIYAL
EFKYDRDLVNIILDTAESKLIDELDTPQNHEHLARLRSYINRRWDNIKPFKMRNLKVTKAIGSCESNRRKYTYRVKGQGKYWSEAGAEGMLRILACIKNR
DLEQWLNSDFEAGKLISLDHKKLLAAARDSLKRRHEIHVGIQHGALTAEAVGGSYLAKLDRMLNHIKY

 

Blast result :
Comments
ISLhe11 is 84% aa similar to ISLcr2.
ISs of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2012) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted);
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).