ISAmde2
- Family ISLre2
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013385 | ND | Ammonifex degensii | Ammonifex degensii KC4 |
DNA section
IS Length : 1584 bp
Ends
IR Length : 19/25
IRL : GTTAGTGTAAAGTTACTATAGGAGTTTTGCAGGAGCTGAGGAGGAAAAAA
IRR : GATCGTGTCAACCTACTGTAGGAGTCTCGATCCCCACCGTCCCTGAGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAGCGCAAAA | CCCGGAGCCG | AGTGGTAGT | 10 |
| ATTATGCTCC | GGGGAGGTT | CACAACAGTT | 9 |
| TTGACACAGA | CCTAACACG | GGGTGTATTG | 9 |
| GGACGGAGAC | CGGGCT | TGCCCAGGTT | 6 |
DNA sequence
GTTAGTGTAAAGTTACTATAGGAGTTTTGCAGGAGCTGAGGAGGAAAAAACGAAGAGAGACCTCACCGTCCTTCGGAGGGACGAGCAGTTGACAATCCAA
CCACTCAGGAGGGTGAGGTCTCTATGCAGATTATAAAGCAGATTTGGGAGAAGTTCCAGAGGGTAGCGGAGCTAACGTTAGAGGTTCTGGAGGAAGGGAT
CGACCTTTTAAGCTTCGAGGAGAAGCTCTGGCAGGAGCTTAAAAGCTTAGGAAAAGAGATCCTGAGGGAAGTTCTGGAGGCAAAAGACGCTTATTTACGG
GAGCACCGGGAAAAGCGGCCTGGCTGGCAAGTAGAGCGGAGGAATGATCCCAAGGAAGTGCTCACCCTTTTCGGTCTGGTCAGCTACAAGCGGACCTATT
ACCGGCACAAAGAGACTGGGGAGCGGGCATACTTAATAGACCGTTTGGTGGGTTACGGGCCGCATACGCGGGTTGACCCTCTGGTCAAGGCACAAGTTTT
AGAAGAAGCAGTAGAGCTTTCTTACCGGAAAAGTGGGGAAAGGGCAGGAAAAGGCAACCCGGAGGTTGTGCTGAGTGGTGAGGCTGTGAAAAAGGTAGTT
CACAACTTCACCCCTGCAGAACTGCCCGAACCACCCAAAGAGAAGCGCCGGGTGAAGGTCCTTTACGTAGAAGCGGACGAAGACCACGTAGCTGGCCAGG
ACAAGAAGAGCCACCTACCTCGTCTTGTTTACATACACGAAGGGAAAGAAGAGGTGGGGAAAGGGCGGTATAAGCTTAAGCGGCCTTACTACCTGGGAGG
GCTTTATCCGGACACGGATGAACTGTGGCTGGATGTTCTAACTTACATTGAGGAGCATTATGAGCTACATGATATTGAGCGGATCTATCTTTGTGGAGAC
GGGGACAGGTGGATAAAGAGGGGTCTGGAGTTTTTACCGAAGAGCGTATTTGTTCTGGACCTTTTCCACCTGGATAAATACCTTGTGGCCGCTTTAGGAA
AGGACAAAGAGGCATATGGAGAGATTTGGGCAGCCTTGAGGCGTGGTGATCGGGTGGGGGTAGAGAAGGTACTGAAGGGAGCGGCGAGGCAGGCGGAGAC
ACCTGGCCGGAGGAAGGCGGTGCGTGATTGTCGGCGCTACATAAATCAGAACTGGGAGGGGATAATGGCTTACAAGCTTTACCCGGAGGCGCAGTTAGGG
GTGAGCGCGGAGGCGCACGTAAGTCATCTCTTCTCGGCACGGTTATCATCCCGACCGATGGCTTGGAGTGCTTGTGGGGTAGACCGGATGGCCCGGTTGC
GGGTGGCGAAGGCGAATGGTGTTTCCTTGCGGGAGCAATATGTAGCCCGATGGAGGGAGGGCTTAAAGGCTTTAGAGATCGATAAGGCAGCCATTGAGGA
AGAGAGGCAGAGGCTAAGGAAAGTATCGGGTGAGGTGTTCGATAATCTTCCCGCATTACGGGGTCCGGTAACGTCATTAACCAGAGCCCTCAAAGCTTTG
AGCCGGAACATCGATCTTCCCTGGTAGTTCGTCCTGGCTCAGGGACGGTGGGGATCGAGACTCCTACAGTAGGTTGACACGATC
CCACTCAGGAGGGTGAGGTCTCTATGCAGATTATAAAGCAGATTTGGGAGAAGTTCCAGAGGGTAGCGGAGCTAACGTTAGAGGTTCTGGAGGAAGGGAT
CGACCTTTTAAGCTTCGAGGAGAAGCTCTGGCAGGAGCTTAAAAGCTTAGGAAAAGAGATCCTGAGGGAAGTTCTGGAGGCAAAAGACGCTTATTTACGG
GAGCACCGGGAAAAGCGGCCTGGCTGGCAAGTAGAGCGGAGGAATGATCCCAAGGAAGTGCTCACCCTTTTCGGTCTGGTCAGCTACAAGCGGACCTATT
ACCGGCACAAAGAGACTGGGGAGCGGGCATACTTAATAGACCGTTTGGTGGGTTACGGGCCGCATACGCGGGTTGACCCTCTGGTCAAGGCACAAGTTTT
AGAAGAAGCAGTAGAGCTTTCTTACCGGAAAAGTGGGGAAAGGGCAGGAAAAGGCAACCCGGAGGTTGTGCTGAGTGGTGAGGCTGTGAAAAAGGTAGTT
CACAACTTCACCCCTGCAGAACTGCCCGAACCACCCAAAGAGAAGCGCCGGGTGAAGGTCCTTTACGTAGAAGCGGACGAAGACCACGTAGCTGGCCAGG
ACAAGAAGAGCCACCTACCTCGTCTTGTTTACATACACGAAGGGAAAGAAGAGGTGGGGAAAGGGCGGTATAAGCTTAAGCGGCCTTACTACCTGGGAGG
GCTTTATCCGGACACGGATGAACTGTGGCTGGATGTTCTAACTTACATTGAGGAGCATTATGAGCTACATGATATTGAGCGGATCTATCTTTGTGGAGAC
GGGGACAGGTGGATAAAGAGGGGTCTGGAGTTTTTACCGAAGAGCGTATTTGTTCTGGACCTTTTCCACCTGGATAAATACCTTGTGGCCGCTTTAGGAA
AGGACAAAGAGGCATATGGAGAGATTTGGGCAGCCTTGAGGCGTGGTGATCGGGTGGGGGTAGAGAAGGTACTGAAGGGAGCGGCGAGGCAGGCGGAGAC
ACCTGGCCGGAGGAAGGCGGTGCGTGATTGTCGGCGCTACATAAATCAGAACTGGGAGGGGATAATGGCTTACAAGCTTTACCCGGAGGCGCAGTTAGGG
GTGAGCGCGGAGGCGCACGTAAGTCATCTCTTCTCGGCACGGTTATCATCCCGACCGATGGCTTGGAGTGCTTGTGGGGTAGACCGGATGGCCCGGTTGC
GGGTGGCGAAGGCGAATGGTGTTTCCTTGCGGGAGCAATATGTAGCCCGATGGAGGGAGGGCTTAAAGGCTTTAGAGATCGATAAGGCAGCCATTGAGGA
AGAGAGGCAGAGGCTAAGGAAAGTATCGGGTGAGGTGTTCGATAATCTTCCCGCATTACGGGGTCCGGTAACGTCATTAACCAGAGCCCTCAAAGCTTTG
AGCCGGAACATCGATCTTCCCTGGTAGTTCGTCCTGGCTCAGGGACGGTGGGGATCGAGACTCCTACAGTAGGTTGACACGATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1404 bp | 467 aa | 124 | 1527 | + | No |
Chemistry : DDE
ORF sequence :
MQIIKQIWEKFQRVAELTLEVLEEGIDLLSFEEKLWQELKSLGKEILREVLEAKDAYLREHREKRPGWQVERRNDPKEVLTLFGLVSYKRTYYRHKETGE
RAYLIDRLVGYGPHTRVDPLVKAQVLEEAVELSYRKSGERAGKGNPEVVLSGEAVKKVVHNFTPAELPEPPKEKRRVKVLYVEADEDHVAGQDKKSHLPR
LVYIHEGKEEVGKGRYKLKRPYYLGGLYPDTDELWLDVLTYIEEHYELHDIERIYLCGDGDRWIKRGLEFLPKSVFVLDLFHLDKYLVAALGKDKEAYGE
IWAALRRGDRVGVEKVLKGAARQAETPGRRKAVRDCRRYINQNWEGIMAYKLYPEAQLGVSAEAHVSHLFSARLSSRPMAWSACGVDRMARLRVAKANGV
SLREQYVARWREGLKALEIDKAAIEEERQRLRKVSGEVFDNLPALRGPVTSLTRALKALSRNIDLPW
RAYLIDRLVGYGPHTRVDPLVKAQVLEEAVELSYRKSGERAGKGNPEVVLSGEAVKKVVHNFTPAELPEPPKEKRRVKVLYVEADEDHVAGQDKKSHLPR
LVYIHEGKEEVGKGRYKLKRPYYLGGLYPDTDELWLDVLTYIEEHYELHDIERIYLCGDGDRWIKRGLEFLPKSVFVLDLFHLDKYLVAALGKDKEAYGE
IWAALRRGDRVGVEKVLKGAARQAETPGRRKAVRDCRRYINQNWEGIMAYKLYPEAQLGVSAEAHVSHLFSARLSSRPMAWSACGVDRMARLRVAKANGV
SLREQYVARWREGLKALEIDKAAIEEERQRLRKVSGEVFDNLPALRGPVTSLTRALKALSRNIDLPW
Blast result :
Comments
ISAmde2 is 60% aa similar to ISNth2.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2012) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted);
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted);
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).