ISDsu1

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_ADLT01000001.1 ND Dialister succinatiphilus
Dialister succinatiphilus YIT 11850
DNA section
IS Length : 1655 bp

Ends


IR Length : 19/24

IRL : CTTAGTGTAAAATTTTACTCGGTAGGGGAATTTTCCAGAAACAATAAAAG
IRR : GTAAGTGTCAAGTTTTCCTCGGTAATTCATTTGACTGTCGATAATTACTA

Insertion site


Left flankDirect repeatRight flankDR Length
GCAGCCATTTTTCTCTTTCTCCCTGCTTT9

DNA sequence

CTTAGTGTAAAATTTTACTCGGTAGGGGAATTTTCCAGAAACAATAAAAGAGGAAAGCCTCCCCTTGAGTGGAAATGTTATAATTTCCGTGCACAAATAA
AACAAAGAAAGGGCTTTCCTCATGGATATTATAACAAAAACTGGAGAAATTTGGAGTGAAAATGCAATAGGAGCAGTCATTTCTATCATCAAGGAATCTG
ATGATTCTATTGAGTCTGAAAGGAAGTTATCTCATTGGCTCGAAGAAATGAACTGTCAGCTTATAGCTATGGCTCTGGAGCGTATTGACGCGGAGCTGTA
TCAGATTTACAGGTCACAGGGGTGGCGTGTGGCGCGCCGGGACTCCCGGACGATTTACTGTCGCTATGGAGAGCTGACCTATACCCGAAGACTTCTGCAG
AAAGATGGGAAGAGCTTCTATCCCCTGGACCGCAAAATGGGCTTTGAGCCCAGAAAACACTACAGCATGGGAATGATTGAGCGGATTGTCGAGGCTGTCG
CAATTACCACATCTCGAAGTGCCAGCGAACTCCTGCAAAGCCTGGCAGGGATTACCATCTCCCATCAGTCCGTTATCTCACTCAAGAATTATGCCGGTAA
GAAGGCAAAGGCTTACGAAAAAGCATTGGCAGAAGAGGAAAAGGTTGAAAAGCCGACACAGCCGGAAATTATAGCGATTGAAGGAGATGGAATTGTACTC
AAGAACAAGGAGAAAGGCGGCGTTTCGGAAGTGCACCGCCTCCAGGTATATGAGGGAGTCAGGAAAAATGGCAACAGGACAGAACTGGTAGGACTTCGCT
GCTTTGCATCTACCAGCAGGAAAGAACTTTTTGCAATGGTAAGAAGCTATCTGCTTGGGCACTATGACCTGTCTAAGACCACAGTGCTGTCGAATGGGGA
TGGAGGGTCCGGATACCAGTTTCAGGACTTTGAGCGAATGGTAGAAGGCTGCCTGGATCACCAGCACTTCCGCGACTGCTATCATGTGCATGAAAAGATC
CGGAACCGGCTCAGCTTCTGCAACAAGGAACTGGTAGACCACATTATCCGAGAGCTTCACCGGACCGACAATATTATGAAACAGATTCCGGTATGGATGG
ACACTGCCCGGAGCTGCGCCCGAACAGAGGCGGATTTGGAGGAGGTAGACAAGCTGCAGAGCTATCTGGAGAGAAATGCTCCCTATATCCCCAGCCTGAG
TCAGCGCGGCATCCATACAGAGATTCACCTGGGGACTGCAGAAACAAATCACCGGTTCTACAGCTATCGCATGAAGCGGCAGGGACGGAGCTGGTCCAGC
GAGGGAATGGAGTGTATGGTTTGGGTATTAACCGCCCGGAAGAATAAAACACTTACAGCAGCTCTCCTGTACCATGCTAATGGGAAAAGCTACAAAAAGA
TGGATAGAGCCATGCACAAGGCTGCGGTGCAGACAGAGCGTAAAATCGCCCAGAATGTCCGGAGTGAGGCGCAGAAGCGTAAAATGAGAAACTATAGGCC
AGGATGCCTGGAGGGGCGTATAGGCGTTTATGGAGCTTCTTCTTCACCAATGGGGAGACTGGCCAGAGCTATACAGAAATACTGATTAAACCGAAGCCAC
GGAGATAGTAATTATCGACAGTCAAATGAATTACCGAGGAAAACTTGACACTTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1464 bp487 aa1221585+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDIITKTGEIWSENAIGAVISIIKESDDSIESERKLSHWLEEMNCQLIAMALERIDAELYQIYRSQGWRVARRDSRTIYCRYGELTYTRRLLQKDGKSFY
PLDRKMGFEPRKHYSMGMIERIVEAVAITTSRSASELLQSLAGITISHQSVISLKNYAGKKAKAYEKALAEEEKVEKPTQPEIIAIEGDGIVLKNKEKGG
VSEVHRLQVYEGVRKNGNRTELVGLRCFASTSRKELFAMVRSYLLGHYDLSKTTVLSNGDGGSGYQFQDFERMVEGCLDHQHFRDCYHVHEKIRNRLSFC
NKELVDHIIRELHRTDNIMKQIPVWMDTARSCARTEADLEEVDKLQSYLERNAPYIPSLSQRGIHTEIHLGTAETNHRFYSYRMKRQGRSWSSEGMECMV
WVLTARKNKTLTAALLYHANGKSYKKMDRAMHKAAVQTERKIAQNVRSEAQKRKMRNYRPGCLEGRIGVYGASSSPMGRLARAIQKY

 

Blast result :
Comments
ISDsu1 is 55% aa similar to ISLhe11.
ISs of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2012) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted);
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).